Abstract: Objective　To evaluate the clinical application significance and methodology characteristic of Sysmex XE-5000 automated hematology analyzer （XE-5000） to count nucleated red blood cell（NRBC）.  Methods　The repeatability，linear range and carryover rate of XE-5000 were evaluated. A total of 391 blood samples were measured, and the alarm and quantitative determination of XE-5000 for NRBC were evaluated. The results were compared with manual microscopy. Results　The XE-5000 results showed good repeatability, linear range and low carryover rate. When NRBC％＞5.0％，it caused false increasing of leucocyte and lymphocyte. The results of manual microscopy were as a gold standard. The correlation was good between the XE-5000 and manual microscopy（r=0.997）. The sensitivity for NRBC was 100.0％，and the specificity was 85.4％.  Conclusions　NRBC counting by XE-5000 is sensitive，accurate and reliable，which is suitable for the clinical detection of NRBC in peripheral blood.

Key words: Nucleated red blood cell, Hematology analyzer, Evaluation