Laboratory Medicine ›› 2026, Vol. 41 ›› Issue (1): 89-94.DOI: 10.3969/j.issn.1673-8640.2026.01.016
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Received:2025-04-09
Revised:2025-07-25
Online:2026-01-30
Published:2026-01-30
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URL: https://www.shjyyx.com/EN/10.3969/j.issn.1673-8640.2026.01.016
| 目标基因 | 引物名 | 引物序列(5'~3') | 产物长度/bp |
|---|---|---|---|
| adk | adk-F | TCATCATCTGCACTTTCCGC | 583 |
| adk-R | CCAGATCAGCGCGAACTTCA | ||
| Fumc | Fumc-F | TCACAGGTCGCCAGCGCTTC | 806 |
| Fumc-R | GTACGCAGCGAAAAAGATTC | ||
| Gyrb | Gyrb-F | TCGGCGACACGGATGACGGC | 911 |
| Gyrb-R | ATCAGGCCTTCACGCGCATC | ||
| Icd | Icd-F | ATGGAAAGTAAAGTAGTTGTTCCGGCACA | 878 |
| Icd-R | GGACGCAGCAGGATCTGTT | ||
| Mdh | Mdh-F | ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG | 932 |
| Mdh-R | TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT | ||
| PurA | PurA-F | TCGGTAACGGTGTTGTGCTG | 816 |
| PurA-R | CATACGGTAAGCCACGCAGA | ||
| RecA | RecA-F | CGCATTCGCTTTACCCTGACC | 780 |
| RecA-R | TCGTCGAAATCTACGGACCGGA | ||
| TEM | TEM-F | ATAAAATTCTTGAAGACGAAA | 1080 |
| TEM-R | GACAGTTACCAATGCTTAATC | ||
| SHV | SHV-F | TGGTTATGCGTTATATTCGCC | 865 |
| SHV-R | GGTTAGCGTTGCCAGTGCT | ||
| CTX-M-1 | CTX1-F | AAAAATCACTGCGCCAGTTC | 415 |
| CTX1-R | AGCTTATTCATCGCCACGTT | ||
| CTX-M-9 | CTX9-F | CAAAGAGAGTGCAACGGATG | 205 |
| CTX9-F | ATTGGAAAGCGTTCATCACC |
| 目标基因 | 引物名 | 引物序列(5'~3') | 产物长度/bp |
|---|---|---|---|
| adk | adk-F | TCATCATCTGCACTTTCCGC | 583 |
| adk-R | CCAGATCAGCGCGAACTTCA | ||
| Fumc | Fumc-F | TCACAGGTCGCCAGCGCTTC | 806 |
| Fumc-R | GTACGCAGCGAAAAAGATTC | ||
| Gyrb | Gyrb-F | TCGGCGACACGGATGACGGC | 911 |
| Gyrb-R | ATCAGGCCTTCACGCGCATC | ||
| Icd | Icd-F | ATGGAAAGTAAAGTAGTTGTTCCGGCACA | 878 |
| Icd-R | GGACGCAGCAGGATCTGTT | ||
| Mdh | Mdh-F | ATGAAAGTCGCAGTCCTCGGCGCTGCTGGCGG | 932 |
| Mdh-R | TTAACGAACTCCTGCCCCAGAGCGATATCTTTCTT | ||
| PurA | PurA-F | TCGGTAACGGTGTTGTGCTG | 816 |
| PurA-R | CATACGGTAAGCCACGCAGA | ||
| RecA | RecA-F | CGCATTCGCTTTACCCTGACC | 780 |
| RecA-R | TCGTCGAAATCTACGGACCGGA | ||
| TEM | TEM-F | ATAAAATTCTTGAAGACGAAA | 1080 |
| TEM-R | GACAGTTACCAATGCTTAATC | ||
| SHV | SHV-F | TGGTTATGCGTTATATTCGCC | 865 |
| SHV-R | GGTTAGCGTTGCCAGTGCT | ||
| CTX-M-1 | CTX1-F | AAAAATCACTGCGCCAGTTC | 415 |
| CTX1-R | AGCTTATTCATCGCCACGTT | ||
| CTX-M-9 | CTX9-F | CAAAGAGAGTGCAACGGATG | 205 |
| CTX9-F | ATTGGAAAGCGTTCATCACC |
| 抗菌药物 | 敏感/株 | 中介/株 | 耐药/株 | 耐药率/% |
|---|---|---|---|---|
| 第1级抗菌药物 | ||||
| 氨苄西林 | 0 | 0 | 100 | 100 |
| 头孢唑林 | 0 | 0 | 100 | 100 |
| 头孢曲松 | 1 | 0 | 99 | 99 |
| 氨苄西林-舒巴坦 | 32 | 25 | 43 | 43 |
| 哌拉西林-他唑巴坦 | 58 | 26 | 16 | 16 |
| 庆大霉素 | 54 | 1 | 45 | 45 |
| 左氧氟沙星 | 15 | 3 | 82 | 82 |
| 复方磺胺甲噁唑 | 29 | 5 | 66 | 66 |
| 呋喃妥因 | 92 | 2 | 6 | 6 |
| 第2级抗菌药物 | ||||
| 头孢呋辛 | 0 | 0 | 100 | 100 |
| 头孢吡肟 | 11 | 44 | 45 | 45 |
| 厄他培南 | 94 | 0 | 6 | 6 |
| 亚胺培南 | 94 | 0 | 6 | 6 |
| 美罗培南 | 94 | 1 | 5 | 5 |
| 妥布霉素 | 50 | 16 | 34 | 34 |
| 阿米卡星 | 84 | 7 | 9 | 9 |
| 头孢西丁 | 75 | 4 | 21 | 21 |
| 四环素 | 20 | 2 | 78 | 78 |
| 第3级抗菌药物 | 95 | 0 | 5 | 5 |
| 头孢他啶-阿维巴坦 | 89 | 2 | 9 | 9 |
| 磷霉素 | ||||
| 第4级抗菌药物 | 50 | 12 | 38 | 38 |
| 氨曲南 | ||||
| 头孢他啶 | 42 | 15 | 43 | 43 |
| 抗菌药物 | 敏感/株 | 中介/株 | 耐药/株 | 耐药率/% |
|---|---|---|---|---|
| 第1级抗菌药物 | ||||
| 氨苄西林 | 0 | 0 | 100 | 100 |
| 头孢唑林 | 0 | 0 | 100 | 100 |
| 头孢曲松 | 1 | 0 | 99 | 99 |
| 氨苄西林-舒巴坦 | 32 | 25 | 43 | 43 |
| 哌拉西林-他唑巴坦 | 58 | 26 | 16 | 16 |
| 庆大霉素 | 54 | 1 | 45 | 45 |
| 左氧氟沙星 | 15 | 3 | 82 | 82 |
| 复方磺胺甲噁唑 | 29 | 5 | 66 | 66 |
| 呋喃妥因 | 92 | 2 | 6 | 6 |
| 第2级抗菌药物 | ||||
| 头孢呋辛 | 0 | 0 | 100 | 100 |
| 头孢吡肟 | 11 | 44 | 45 | 45 |
| 厄他培南 | 94 | 0 | 6 | 6 |
| 亚胺培南 | 94 | 0 | 6 | 6 |
| 美罗培南 | 94 | 1 | 5 | 5 |
| 妥布霉素 | 50 | 16 | 34 | 34 |
| 阿米卡星 | 84 | 7 | 9 | 9 |
| 头孢西丁 | 75 | 4 | 21 | 21 |
| 四环素 | 20 | 2 | 78 | 78 |
| 第3级抗菌药物 | 95 | 0 | 5 | 5 |
| 头孢他啶-阿维巴坦 | 89 | 2 | 9 | 9 |
| 磷霉素 | ||||
| 第4级抗菌药物 | 50 | 12 | 38 | 38 |
| 氨曲南 | ||||
| 头孢他啶 | 42 | 15 | 43 | 43 |
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