Abstract: Objective　To clarify the distribution of the von Willebrand factor (vWF) gene intron 19 MspI polymorphism among Han population in Kunming，and investigate the relationship of vWF gene intron 19 MspI polymorphism with acute ischemic cerebral infarction(AICI). Methods　A total of 119 patients with AICI and 117 healthy controls were enrolled. Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was used to determine the vWF gene intron 19 MspI polymorphism. Results　Among the Han population in Kunming, the vWF gene intron 19 MspI M+/M+，M+/M-and M-/M-genotype frequencies were 0.034，0.470 and 0.496. M+ and M- allele frequencies were 0.269 and 0.731. M+/M+，M + / M-and M-/ M- genotype frequencies in AICI group were 0.059, 0.286 and 0.655，and M + and M- allele frequencies were 0.202 and 0.798, respectively. M+/M- genotype frequencies in AICI group were lower than those in control group, and M-/M- genotype frequencies were higher than those in control group. The frequencies between AICI and control groups had significant difference (P<0.05). Conclusions　The vWF gene intron 19 MspⅠ restriction site genotype distributions among Han population in Kunming are quite different comparing with Gaucasian populations，and the other ethnic populations in Kunming has some differences. Acute cerebral infarction may be associated with vWF gene intron 19 MspⅠ polymorphisms.

Key words: Acute cerebral infarction, Von Willebrand factor, Gene polymorphism, MspI restriction site