›› 2012, Vol. 27 ›› Issue (5): 408-411.

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The construction and confirmation of HIV-1 electrochemically active–inactive switching molecular beacon

  

  1. 1. Laboratory of Point-of-care Testing,Clinical Laboratory Center,Nanfang Hospital,Southern Medical University,Guangdong Guangzhou 510515,China;2.Shenzhen International Travel Health Care Center, Guangdong Shenzhen 518033,China;3. Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangdong Guangzhou 510515, China
  • Received:2012-05-10 Online:2012-05-30 Published:2012-05-10

Abstract: Objective To construct human immunodeficiency virus-1 (HIV-1) electrochemically active-inactive switching molecular beacon(CAs-MB) and confirm its structure and function.   Methods CAs-MB targeted conserved region of HIV-1 gag gene was prepared and purified. The structure was confirmed by Fourier transform-infrared spectroscopy (FT-IR) and cyclic voltammetry (CV), while its function of recognizing targets was evaluated by differential pulse voltammetry (DPV). Results The variations of FT-IR spectra of free carminicacid (CA) and CAs-MB indicated that CA had covalently linked onto the ends of MB. The results of CV proved that both ON and OFF statuses of CAs-MB were existing. DPV results illustrated that CAs-MB was effective in signaling the presence of complementary target and discriminating targets in electrochemical gene biosensor that differ by a single nucleotide. Conclusions CAs-MB opens a new detective technique and can be expanded to electrochemical gene biosensor to perform HIV-1 gag gene specificity detection.

Key words: Human immunodeficiency virus-1, Molecular beacon, Electrochemical gene biosensor