Roles of serum CA125，TRAP1 and HO-1 in efficacy evaluation of targeted therapy combined with chemotherapy for patients with advanced non-small cell lung cancer

doi:10.3969/j.issn.1673-8640.2025.10.007

Abstract

Abstract:

Objective To investigate the roles of serum carbohydrate antigen（CA） 125，tumor necrosis factor receptor-associated protein 1（TRAP1） and heme oxygenase-1（HO-1） in the efficacy evaluation of targeted therapy combined with chemotherapy in patients with advanced non-small cell lung cancer（NSCLC）. Methods A total of 100 patients with advanced NSCLC in the Second Hospital of Qinhuangdao from June 2021 to June 2023 were enrolled. All the patients were given erlotinib（squamous carcinoma）/gefitinib（adenocarcinoma） targeted therapy combined with pemetrexed+cisplatin chemotherapy. The patients were classified into effective group（53 cases） and ineffective group（47 cases） according to therapeutic effect. The clinical data were collected，and the levels of serum CA125，TRAP1 and HO-1 were determined. Spearman correlation analysis was used to evaluate the correlation between the levels of CA125，TRAP1 and HO-1 and therapeutic effect. Logistic regression analysis was used to evaluate the influencing factors of the therapeutic effect of targeted therapy combined with chemotherapy in patients with advanced NSCLC. Receiver operating characteristic（ROC） curve was used to evaluate the efficacy of serum CA125，TRAP1 and HO-1 in judging the therapeutic effect of targeted therapy combined with chemotherapy in patients with advanced NSCLC. Results There was statistical significance in the degree of differentiation between ineffective group and effective group（P<0.05），and there was no statistical significance in age，gender，smoking history，pathological type and TNM stage（P>0.05）. The levels of serum CA125，TRAP1 and HO-1 in ineffective group were higher than those in effective group（P<0.05）. The levels of serum CA125，TRAP1 and HO-1 in patients with advanced NSCLC were negatively correlated with the therapeutic effect of targeted therapy combined with chemotherapy（r_s values were -0.522，-0.467 and -0.493，respectively，P<0.05）. The degree of differentiation，CA125，TRAP1 and HO-1 were all risk factors for the ineffectiveness of targeted therapy combined with chemotherapy in advanced NSCLC [odds ratios（OR） were 3.664，4.307，4.139 and 3.458，95% confidence intervals（CI） were 1.838-7.304，1.883-9.849，1.985-8.632 and 1.426-8.386，respectively，P<0.05]. The areas under curves（AUC） of single and combined determinations of CA125，TRAP1 and HO-1 for judging the ineffectiveness of targeted therapy combined with chemotherapy in patients with advanced NSCLC were 0.756，0.784，0.770 and 0.878，respectively. Conclusions Serum CA125，TRAP1 and HO-1 have certain predictive value in the efficacy evaluation of targeted therapy combined with chemotherapy in patients with advanced NSCLC，which may be used as the indicators for therapeutic effect prediction.

Key words: Carbohydrate antigen 125, Tumor necrosis factor receptor-associated protein 1, Heme oxygenase-1, Non-small cell lung cancer, Targeted therapy combined with chemotherapy

CLC Number:

R446.1

LU Xiuming, CHAI Baoying, CAO Yingqi, LIU Xin, YANG Lin. Roles of serum CA125，TRAP1 and HO-1 in efficacy evaluation of targeted therapy combined with chemotherapy for patients with advanced non-small cell lung cancer[J]. Laboratory Medicine, 2025, 40(10): 972-977.

Figures/Tables 5

References 23

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组别	例数	年龄/岁	性别			病理类型
组别	例数	年龄/岁	男/[例（%）]	女/[例（%）]		鳞癌/[例（%）]	腺癌/ [例（%）]
无效组	47	53.34±5.94	27（57.45）	20（42.55）		20（42.55）	27（57.45）
有效组	53	53.06±6.11	28（52.83）	25（47.17）		23（43.40）	30（56.60）
统计值		0.232	0.215			0.007
P值		0.817	0.643			0.932
组别	吸烟史/[例（%）]		TNM分期			分化程度
组别	吸烟史/[例（%）]		Ⅲb期/ [例（%）]		Ⅳ期/ [例（%）]	低分化/ [例（%）]	中高分化/ [例（%）]
无效组	24（51.06）		31（65.96）		16（34.04）	28（59.57）	19（40.43）
有效组	21（39.62）		33（62.26）		20（37.74）	21（39.62）	32（60.38）
统计值	1.317		0.147			3.968
P值	0.251		0.701			0.046

组别	例数	年龄/岁	性别			病理类型
组别	例数	年龄/岁	男/[例（%）]	女/[例（%）]		鳞癌/[例（%）]	腺癌/ [例（%）]
无效组	47	53.34±5.94	27（57.45）	20（42.55）		20（42.55）	27（57.45）
有效组	53	53.06±6.11	28（52.83）	25（47.17）		23（43.40）	30（56.60）
统计值		0.232	0.215			0.007
P值		0.817	0.643			0.932
组别	吸烟史/[例（%）]		TNM分期			分化程度
组别	吸烟史/[例（%）]		Ⅲb期/ [例（%）]		Ⅳ期/ [例（%）]	低分化/ [例（%）]	中高分化/ [例（%）]
无效组	24（51.06）		31（65.96）		16（34.04）	28（59.57）	19（40.43）
有效组	21（39.62）		33（62.26）		20（37.74）	21（39.62）	32（60.38）
统计值	1.317		0.147			3.968
P值	0.251		0.701			0.046

组别	例数	CA125/（U·mL^-1）	TRAP1/（ng·L^-1）	HO-1/（μg·L^-1）
无效组	47	82.33±28.41	64.28±6.79	14.62±2.31
有效组	53	51.42±11.57	51.68±5.17	10.08±2.19
t值		7.273	10.507	10.084
P值		<0.001	<0.001	<0.001

组别	例数	CA125/（U·mL^-1）	TRAP1/（ng·L^-1）	HO-1/（μg·L^-1）
无效组	47	82.33±28.41	64.28±6.79	14.62±2.31
有效组	53	51.42±11.57	51.68±5.17	10.08±2.19
t值		7.273	10.507	10.084
P值		<0.001	<0.001	<0.001

项目	β值	标准误	Wald值	P值	OR值（95%CI）
分化程度	1.299	0.352	13.609	<0.001	3.664（1.838~7.304）
CA125	1.460	0.422	11.974	0.001	4.307（1.883~9.849）
TRAP1	1.420	0.375	14.348	<0.001	4.139（1.985~8.632）
HO-1	1.241	0.452	7.534	0.006	3.458（1.426~8.386）