Abstract: Objective To analyze the human papillomavirus（HPV） infection status and the subtype distribution of cervical cancer patients in Anhui,and to investigate the correlation between HPV infection and clinical pathological characteristics of cervical cancer patients and the relationship between HPV DNA copy number changes and the efficiency of radiotherapy. Methods A total of 230 patients with cervical cancer were enrolled. Real-time fluorescence quantitative polymerase chain reaction（PCR） was used to determine 15 common high-risk HPV DNA subtypes,and the positive rate and subtype distribution characteristics of HPV infection in patients with cervical cancer were analyzed. The relationship between positive rate and subtype distribution in ages and clinical stages and the relationship between the copy number changes of HPV DNA and the efficiency of radiotherapy in patients with cervical cancer were evaluated. Results The positive rate of HPV infection in 230 patients with cervical cancer was 83.0%,the single subtype positive rate was 73.2%,and the multiple subtype positive rate was 26.8%. The subtypes of HPV infection in cervical cancer patients were mainly HPV 16,18 and 58. HPV infection was not correlated with ages,but it was related to clinical stages（P<0.05）. The later clinical stages made lower infection rates. The single subtype infection rate after radiotherapy was higher than that before radiotherapy,the radiotherapy efficiency of single subtype infection was higher than that of multiple subtype infection,and the copy number in invalid group would increase. Conclusions HPV infection in patients with cervical cancer in Anhui is dominated by HPV 16,18 and 58. HPV subtype and copy number of HPV infection could be used as the indicators for evaluating the efficiency of radiotherapy.

Key words: Human papillomavirus, Cervical cancer, Genotype, Radiotherapy