Correlations of Lp-PLA2 activity and gene polymorphism with ischemic cerebral stroke

doi:10.3969/j.issn.1673-8640.2016.010.007

Abstract

Abstract:

Objective To investigate the correlations of lipoprotein-associated phospholipase A2（Lp-PLA2）activity and its gene locus rs1051931（Ala379Val） polymorphism with ischemic cerebral stroke（ICS）. Methods Polymerase chain reaction（PCR） and direct sequencing assay were used to determine rs1051931 polymorphism in 480 patients with ICS and 630 healthy subjects（healthy control group）. Plasma Lp-PLA2 activity,total cholesterol（TC）,triglyceride（TG）,high-density lipoprotein cholesterol（HDL-C）,low-density lipoprotein cholesterol（LDL-C） and glucose（Glu） were determined simultaneously.Results The proportion of males,the proportion of hypertension,Lp-PLA2 activity,TG,TC,HDL-C,LDL-C and Glu in ICS group were higher than those in healthy control group（P<0.05）. The differences of ages and body mass index（BMI） between the 2 groups were not statistically significant（P>0.05）. ICS group had high levels of traditional vascular risk factors,including relatively-high blood pressure and LDL-C and relatively-low HDL-C. After the calibration of traditional vascular risk factors,the highest and lowest quartiles of Lp-PLA2 activity were analyzed {odds ratio（OR）[95% confidence interval（CI）] =2.25（1.23-9.96）}. Lp-PLA2 activities [median（quartile）] were 56.44（27.56-87.84）nmol/（mL·min） in genotype CC,43.26（26.73-78.51）nmol/（mL·min） in genotype CT and 38.24（22.98-62.84）nmol/（mL·min） in genotype TT（P<0.05）. By Binary Logistic regression analysis,Lp-PLA2 genotype was used as an independent variable,the frequency of genotype CC in ICS group was high [OR（95%CI）=1.707（1.055-2.761）]. Conclusions Lp-PLA2 activity in ICS group is higher than that in healthy control group,and its polymorphism is correlated with ICS. Genotype CC is a risk factor for ICS.

Key words: Lipoprotein-associated phospholipase A2, Single nucleotide polymorphism, Ischemic cerebral stroke

CLC Number:

R446.1

LI Suliang, YE Yun. Correlations of Lp-PLA2 activity and gene polymorphism with ischemic cerebral stroke[J]. Laboratory Medicine, 2016, 31(10): 869-873.

Figures/Tables 6

References 15

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组别	例数	年龄	男性[例（%）]	BMI（kg/㎡）	高血压[例（%）]	TC（mmol/L）
ICS组	480	57±13	280（58.3）	24.5±3.7	335（69.7）	6.15（4.56~7.21）
正常对照组	630	56±13	256（40.6）	24.1±3.6	212（33.6）	5.67（3.96~6.61）
统计值		0.348	10.177	1.691	90.045	-2.561
P值		0.729	0.001	0.096	0.001	0.01

组别	TG（mmol/L）	HDL-C（mmol/L）	LDL-C（mmol/L）	Glu（mmol/L）	Lp-PLA2[nmol/（mL·min）]
ICS组	1.88（1.02~3.18）	1.0（0.71~1.32）	2.86（1.77~3.42）	6.52（5.02~8.78）	45.67（22.98~87.84）
正常对照组	1.51（0.89~2.75）	1.2（0.90~1.58）	2.13（1.56~3.27）	5.48（3.89~6.71）	19.77（5.68~40.64）
统计值	-2.784	-5.366	-2.616	-2.698	-8.102
P值	0.005	0.001	0.009	0.001	0.001

组别	例数	年龄	男性[例（%）]	BMI（kg/㎡）	高血压[例（%）]	TC（mmol/L）
ICS组	480	57±13	280（58.3）	24.5±3.7	335（69.7）	6.15（4.56~7.21）
正常对照组	630	56±13	256（40.6）	24.1±3.6	212（33.6）	5.67（3.96~6.61）
统计值		0.348	10.177	1.691	90.045	-2.561
P值		0.729	0.001	0.096	0.001	0.01

组别	TG（mmol/L）	HDL-C（mmol/L）	LDL-C（mmol/L）	Glu（mmol/L）	Lp-PLA2[nmol/（mL·min）]
ICS组	1.88（1.02~3.18）	1.0（0.71~1.32）	2.86（1.77~3.42）	6.52（5.02~8.78）	45.67（22.98~87.84）
正常对照组	1.51（0.89~2.75）	1.2（0.90~1.58）	2.13（1.56~3.27）	5.48（3.89~6.71）	19.77（5.68~40.64）
统计值	-2.784	-5.366	-2.616	-2.698	-8.102
P值	0.005	0.001	0.009	0.001	0.001

组别	Lp-PLA2[nmol/（mL·min）]	例数	模型1	模型2	模型3
Q1组	22.98~<45.35	102	1.00	1.00	1.00
Q2组	45.35~<53.62	135	1.44（0.61~2.32）	1.36（0.79~1.67）	1.24（0.62~1.58）
Q3组	53.62~<66.73	140	1.56（0.89~2.37）	1.47（0.68~1.95）	1.27（0.51~1.14）
Q4组	66.73~<87.84	103	6.52（2.34~22.05）	3.12（1.67~12.26）	2.25（1.23~9.96）