A comparative study of three determination methods on the effect of apoE genotyping

doi:10.3969/j.issn.1673-8640.2013.09.013

Abstract

Abstract:

Objective To evaluate the 3 determination methods [multiplex amplification refractory mutation system polymerase chain reaction (Multi-ARMS PCR), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP)] on the effect of apolipoprotein E (apo E) genotyping. Methods apo E genotypes of 518 healthy subjects were analyzed by Multi-ARMS PCR, PCR-RFLP and PCR-SSCP respectively, and the results were confirmed by DNA sequencing. Results The results of the 3 determination methods were not entirely consistent with the results of DNA sequencing (Multi-ARMS PCR Kappa=0.964, PCR-RFLP Kappa=0.991, PCR-SSCP Kappa=0.913, and Kappa was closer to 1, the consistency was better). Conclusions Among the 3 determination methods, PCR-RFLP is the most reliable one for apoE genotyping.

Key words: Apolipoprotein E, Genotyping, Determination method, Evaluation, DNA sequencing

CLC Number:

Q503

XIONG Yu,QIAN Shiyun.. A comparative study of three determination methods on the effect of apoE genotyping[J]. , 2013, 28(9): 789-792.

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