Abstract: Objective　To study the influence of different degrees of sample hemolysis on electrochemiluminescence immunoassay（ECLIA） results. Methods　The blood samples were manually intervened into different degrees of hemolysis, and were used for detecting and analyzing the concentrations of 28 indices such as thyroid function, tumor markers, endocrine hormones and so on in various groups of samples. Results　Sample hemolysis can reduce the concentration of insulin（INS），and the concentration of folic acid （FA） and neuron-specific enolase （NSE） were increased. Severe hemolysis can make the results to have a higher trend on determination of ferritin，however, it was not statistically significant, and the determination results of other indices was not affected by hemolysis. Conclusions　The results of most hemolytic samples except certain indices are not affected. The determinations of INS, FA and NSE should absolutely avoid hemolytic samples to ensure the accuracy of results.

Key words: Hemolysis, Insulin, Folic acid, Neuron-specific enolase, Ferritin, Electrochemiluminescence immunoassay