Investigation on the interference of bilirubin and hemoglobin for different urinary albumin measurements

Abstract

Abstract: Objective　To investigate the interference of bilirubin（Bil） and hemoglobin（Hb） in urine on different urinary albumin measurements. Methods　Bil and Hb，as interferents，performed interference test by 3 different urinary albumin measurements：immuno-nephelometry，immuno-turbidimetry and immuno-colloidal gold method，respectively. Results　Bil and Hb with different concentrations could affect the results of urinary albumin by all the 3 measurements，and the interferents rate increased with the increasing concentration of interferents. Hb mainly generated negative interference by the 3 methods，while the interferences of Bil by immuno-nephelometry and immuno-turbidimetry were positive，and by immuno-colloidal gold method was negative. In the 3 methods，when urine occult blood was "1+"，the absolute value of interference rate of Hb was 6.72%-17.43%. When urine Bil was "1+"，the absolute value of interference rate was 7.89%-22.94%.Conclusions　Bil and Hb could generate interference on urinary albumin measurements. When detecting urinary albumin from the patients with suspected high concentrations of Bil and Hb in urine，the interference factors should be considered.

Key words: Albumin, Bilirubin, Hemoglobin, Urine, Interference test

LIU Rui;LI Gang;CUI Xiaofan . Investigation on the interference of bilirubin and hemoglobin for different urinary albumin measurements[J]. , 2012, 27(7): 564-567.

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