Abstract: Objective　To study the status of 21 genotypes of human papillomavirus (HPV) infection in women of western Guangdong and the relationship between HPV genotypes and different grade cervical lesion. Methods　A total of 5 556 cases were detected for HPV DNA types by DNA flow through hybridization and gene chip (HybriMax)， and cervical biopsies were used for pathological diagnosis in 677 cases. Results　Among 5 556 cases， 901 cases were HPV positive (16.2%). The HPV subtype infection rate in top 5 were HPV 52， 16， 58，53 and 11 in turn.There were statistical significance among the HPV positive rates in different age groups（P<0.05）.Old women (≥51 years old) showed the highest HPV infection rate. The constituent ratio of high risk HPV(HRHPV) types was the highest in 4150 years old group（P<0.05）. The detection rate of HPV increased with the severity degree of cervical lesion（P<0.05）.Multiple HPV type infection rate was the highest in high grade squamous intraepithelial lesion (HSIL)group（P<0.05）. The detection rates of HPV16, 58 and 33 increased with the severity degree of cervical lesion（P<0.05）. Compared with low grade squamous intraepithelial lesion (LSIL )group， the odds ratios(OR)of HSIL group were 3.391(2.038－5.643),2.409(1.188－4.888) and 2.362(1.081－5.161)respectively. Conclusions　The popular HPV subtypes in western Guangdong are HPV 52， 16， 58，53 and 11. The detection of HPV genotypes is of great significance for early diagnosis and prognosis of cervical lesion. HPV 16， 58 and 33 are more oncogenic than other HPV types. HPV16 is the most oncogenic genotype.

Key words: Human papillomavirus, Cervical lesion, Gene subtype