Research progress of electrochemical aptasensors for the determination of biomarkers

doi:10.3969/j.issn.1673-8640.2022.05.016

Abstract

Abstract:

Aptamers have attracted more and more attention in clinical determination due to their specific recognition of disease biomarkers. At present,there have been many research reports,using aptamers to identify biomarkers to form determination method which has detectable signals. With the development of aptamer biosensors,its application prospects are also revealed. The combination of aptamers and functional nanomaterials can improve determination sensitivity and reduce determination limit. This review introduces the method of aptamer nanotechnology to construct electrochemical biosensors and how to improve the performance of biosensors. The application of biosensors in the determination of disease biomarkers is exemplified. The application prospects and challenges of aptamer biosensors are discussed in this review as well.

Key words: Aptamer, Biomarker, Electrochemical biosensor

FENG Xueqing, JU Yi, LI Qing, JIN Zhonggan. Research progress of electrochemical aptasensors for the determination of biomarkers[J]. Laboratory Medicine, 2022, 37(5): 477-482.

Figures/Tables 3

References 38

[1]	LABIB M, SARGENT E H, KELLEY S O. Electrochemical methods for the analysis of clinically relevant biomolecules[J]. Chem Rev, 2016, 116(16):9001-9090. DOI URL
[2]	KELLEY S O. What are clinically relevant levels of cellular and biomolecular analytes[J]. ACS Sens, 2017, 2(2):193-197. DOI URL
[3]	KELLEY S O, MIRKIN C A, WALT D R,et al.
	Advancing the speed,sensitivity and accuracy of biomolecular detection using multi-length-scale engineering[J]. Nat Nanotechnol, 2014, 9(12):969-980. DOI URL
[4]	FAN C, PLAXCO K W, HEEGER A J. Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA[J]. Proc Natl Acad Sci U S A, 2003, 100(16):9134-9137. DOI URL
[5]	SOLEYMANI L, FANG Z, SARGENT E H, et al. Programming the detection limits of biosensors through controlled nanostructuring[J]. Nat Nanotechnol, 2009, 4(12):844-848. DOI URL
[6]	BELL N A, KEYSER U F. Digitally encoded DNA nanostructures for multiplexed,single-molecule protein sensing with nanopores[J]. Nat Nanotechnol, 2016, 11(7):645-651. DOI URL
[7]	DE LUNA P, MAHSHID S S, DAS J, et al. High-curvature nanostructuring enhances probe display for biomolecular detection[J]. Nano Lett, 2017, 17(2):1289-1295. DOI URL
[8]	PEI H, ZUO X, ZHU D, et al. Functional DNA nanostructures for theranostic applications[J]. Acc Chem Res, 2014, 47(2):550-559. DOI URL
[9]	YAN S R, FOROUGHI M M, SAFAEI M, et al. A review:recent advances in ultrasensitive and highly specific recognition aptasensors with various detection strategies[J]. Int J Biol Macromol, 2020, 155:184-207. DOI URL
[10]	WADHERA T, KAKKAR D, WADHWA G, et al. Recent advances and progress in development of the field effect transistor biosensor:a review[J]. J Electron Mater, 2019, 48(12):7635-7646. DOI URL
[11]	YU L, LI N. Noble metal nanoparticles-based colorimetric biosensor for visual quantification:a mini review[J]. Chemosensors, 2019, 7(4):53. DOI URL
[12]	MOUTSIOPOULOU A, BROYLES D, DIKICI E, et al. Molecular aptamer beacons and their applications in sensing,imaging,and diagnostics[J]. Small, 2019, 15(35):e1902248.
[13]	HUANG S, CHEN M, XUAN Z, et al. Aptamer-based electrochemical sensors for rapid detection of veterinary drug residues[J]. Int J Electrochem Sci, 2020, 15:4102-4116.
[14]	ALKHAMIS O, CANOURA J, YU H, et al. Innovative engineering and sensing strategies for aptamer-based small-molecule detection[J]. Trends Analyt Chem, 2019, 121:115699. DOI URL
[15]	JONES M R, SEEMAN N C, MIRKIN C A. Nanomaterials. Programmable materials and the nature of the DNA bond[J]. Science, 2015, 347(6224):1260901. DOI URL
[16]	ZHANG L, LI Z, ZHOU X, et al. Hybridization performance of DNA/mercaptohexanol mixed monolayers on electrodeposited nanoAu and rough Au surfaces[J]. J Electroanal Chem, 2015, 757:203-209. DOI URL
[17]	KIMURA-SUDA H, PETROVYKH D Y, TARLOV M J, et al. Base-dependent competitive adsorption of single-stranded DNA on gold[J]. J Am Chem Soc, 2003, 125(30):9014-9015. DOI URL
[18]	WANG S, CAI X, WANG L, et al. DNA orientation-specific adhesion and patterning of living mammalian cells on self-assembled DNA monolayers[J]. Chem Sci, 2016, 7(4):2722-2727. DOI URL
[19]	XIE D, FENG X Q, HU X L, et al. Probing mannose-binding proteins that express on live cells and pathogens with a diffusion-to-surface ratiometric graphene electrosensor[J]. ACS Appl Mater Interfaces, 2016, 8(38):25137-25141. DOI URL
[20]	WAHIBA M, FENG X Q, ZANG Y, et al. A supramolecular pyrenyl glycoside-coated 2D MoS2 composite electrode for selective cell capture[J]. Chem Commun, 2016, 52(78):11689-11692. DOI URL
[21]	BESANT J D, DAS J, BURGESS I B, et al. Ultrasensitive visual read-out of nucleic acids using electrocatalytic fluid displacement[J]. Nat Commun, 2015, 6:6978. DOI URL
[22]	CHEN X, ZHOU G, SONG P, et al. Ultrasensitive electrochemical detection of prostate-specific antigen by using antibodies anchored on a DNA nanostructural scaffold[J]. Anal Chem, 2014, 86(15):7337-7342. DOI URL
[23]	PEI H, LU N, WEN Y, et al. A DNA nanostructure-based biomolecular probe carrier platform for electrochemical biosensing[J]. Adv Mater, 2010, 22(42):4754-4758. DOI URL
[24]	MAZLOUM-ARDAKANI M, TAVAKOLIAN-ARDAKANI Z, SAHRAEI N, et al. Fabrication of an ultrasensitive and selective electrochemical aptasensor to detect carcinoembryonic antigen by using a new nanocomposite[J]. Biosens Bioelectron, 2019, 129:1-6. DOI URL
[25]	HONG B, ZU Y. Detecting circulating tumor cells:current challenges and new trends[J]. Theranostics, 2013, 3(6):377-394. DOI URL
[26]	YANG Y, FU Y, SU H, et al. Sensitive detection of MCF-7 human breast cancer cells by using a novel DNA-labeled sandwich electrochemical biosensor[J]. Biosens Bioelectron, 2018, 122:175-182. DOI URL
[27]	WANG S, ZHANG L, WAN S, et al. Aptasensor with expanded nucleotide using DNA nanotetrahedra for electrochemical detection of cancerous exosomes[J]. ACS Nano, 2017, 11(4):3943-3949. DOI URL
[28]	ZHOU Q, RAHIMIAN A, SON K, et al. Development of an aptasensor for electrochemical detection of exosomes[J]. Methods, 2016, 97:88-93. DOI URL
[29]	QIAO X, LI K, XU J, et al. Novel electrochemical sensing platform for ultrasensitive detection of cardiac troponin I based on aptamer-MoS2 nanoconjugates[J]. Biosens Bioelectron, 2018, 113:142-147. DOI URL
[30]	ZHANG Y, FIGUEROA-MIRANDA G, ZAFIU C, et al. Amperometric aptasensor for amyloid-β oligomer detection by optimized stem-loop structures with an adjustable detection range[J]. ACS Sens, 2019, 4(11):3042-3050. DOI URL
[31]	DAI Y, SOMOZA R A, WANG L, et al. Exploring the trans-cleavage activity of CRISPR-Cas12a(cpf1)for the development of a universal electrochemical biosensor[J]. Angew Chem Int Ed Engl, 2019, 58(48):17399-17405. DOI URL
[32]	HE X P, ZHU B W, ZANG Y, et al. Dynamic tracking of pathogenic receptor expression of live cells using pyrenyl glycoanthraquinone-decorated graphene electrodes[J]. Chem Sci, 2015, 6(3):1996-2001. DOI URL
[33]	LI Z, DENG S S, ZANG Y, et al. Capturing intercellular sugar-mediated ligand-receptor recognitions via a simple yet highly biospecific interfacial system[J]. Sci Rep, 2013, 3:2293. DOI URL
[34]	WANG F, LIU L S, LAU C H, et al. Synthetic α-L-threose nucleic acids targeting BcL-2 show gene silencing and in vivo antitumor activity for cancer therapy[J]. ACS Appl Mater Interfaces, 2019, 11(42):38510-38518. DOI URL
[35]	ZHANG H, LI F, LI X F, et al. Yoctomole detection of proteins using solid phase binding-induced DNA assembly[J]. Methods, 2013, 64(3):322-330. DOI URL
[36]	LIU L S, LEUNG H M, TAM D Y, et al. α-L-threose nucleic acids as biocompatible antisense oligonucleotides for suppressing gene expression in living cells[J]. ACS Appl Mater Interfaces, 2018, 10(11):9736-9743. DOI URL
[37]	NOVIANA E, MCCORD C P, CLARK K M, et al. Electrochemical paper-based devices:sensing approaches and progress toward practical applications[J]. Lab Chip, 2020, 20(1):9-34. DOI URL
[38]	WANG L, WU A, WEI G. Graphene-based aptasensors:from molecule-interface interactions to sensor design and biomedical diagnostics[J]. Analyst, 2018, 143(7):1526-1543. DOI URL