Expression of TLR-4 and HMGB1 in peripheral blood of children with SLE and its clinical significance

doi:10.3969/j.issn.1673-8640.2020.11.014

Abstract

Abstract:

Objective To investigate the roles of Toll-like receptor 4（TLR-4） and high mobility group protein B1（HMGB1） in peripheral blood of children with systemic lupus erythematosus（SLE）. Methods Totally,40 children with SLE（SLE group） and 15 healthy children（control group） were enrolled. SLE group was classified into active group（20 cases） with systemic lupus erythematosus disease activity index（SLEDAI） score ≥10 and stable group（20 cases） with SLEDAI score <10,and according to presence or absence of nephritis,they were classified into nephritis group（24 cases） and non-nephritis group（16 cases）. Serum interleukin（IL）-17,IL-23,HMGB1 and TLR-4 were determined. Pearson correlation analysis was used to analyze the correlations. Results Serum levels of IL-17,IL-23,HMGB1 and TLR-4 in SLE group were higher than those in control group（P<0.05）. Serum levels of IL-17,IL-23,HMGB1 and TLR-4 in active group were higher than those in stable group（P<0.05）. Serum levels of IL-17,IL-23,HMGB1 and TLR-4 in nephritis group were higher than those in non-nephritis group（P<0.05）. HMGB1 was positively correlated with TLR-4,IL-17 and IL-23（r=0.424,0.563,0.498 and 0.745,P<0.05）. IL-17 was positively correlated with SLEDAI score（r =0.726,P<0.05）. IL-23 was positively correlated with SLEDAI score,IgM,IgG and C-reactive protein（CRP）（r=0.615,0.321,0.465 and 0.587,P<0.05）. There was a negative correlation between C3 and C4（r =-0.461 and -0.399,P<0.05）. HMGB1 was positively correlated with anti-double-stranded DNA（dsDNA） antibody,anti-Sm antibody,IgM and IgG（r=0.727,0.659,0.487 and 0.550,P<0.05）. There was a negative correlation with C3（r=-0.367,P<0.05）. TLR-4 was positively correlated with SLEDAI score,anti-dsDNA antibody,CRP and IgM（r=0.762,0.721,0.614 and 0.636,P<0.05）. There was a negative correlation between C3 and C4（r=-0.520 and -0.434,P<0.05）. Conclusions TLR-4 and HMGB1 are related to the occurrence and development of SLE in children,and they also play roles in renal injury in children with SLE.

Key words: Toll-like receptor 4, High mobility group protein B1, Systemic lupus erythematosus

CLC Number:

R521

LÜ Yingxia, YANG Junmei, ZHENG Lijuan, HUANG Qinghua. Expression of TLR-4 and HMGB1 in peripheral blood of children with SLE and its clinical significance[J]. Laboratory Medicine, 2020, 35(11): 1143-1146.

Figures/Tables 3

References 15

[1]	黄莉,盛伟松. 高迁移率族蛋白B1及Toll样受体4在儿童系统性红斑狼疮中的临床意义[J]. 中华风湿病学杂志,2019,23(6):365-368.
[2]	沈小雁,薛峰,陈晓鸿,等. 系统性红斑狼疮患者外周血单一核细胞Toll样受体4及2mRNA表达的研究[J]. 中华皮肤科杂志,2004,37(10):572-574.
[3]	潘舒月,朱勇,刘怡,等. 高迁移率族蛋白B1及其糖基化终产物受体在系统性红斑狼疮患者中的变化及其临床意义[J]. 重庆医学,2016,45(21):2922-2925.
[4]	黄华,崔明辰. 儿科学[M]. 8版. 北京:人民卫生出版社,2018.
[5]	ALBAYATI Z,ALYAMI A,ALOMAR S,et al.The influence of cytomegalovirus on expression of HLA-G and its ligand KIR2DL4 by human peripheral blood leucocyte subsets[J]. Scand J Immunol,2017,86(5):396-407.
[6]	麻贞贞,赵萍,吕继彩,等. 系统性红斑狼疮患者外周血单个核细胞中微RNA-223及核苷酸结合寡聚化结构域样受体家族蛋白3炎性小体的表达及临床意义[J]. 中华风湿病学杂志,2019,23(1):10-14.
[7]	张梦云,青玉凤,潘舒月,等. 端粒重复序列结合蛋白1与2在系统性红斑狼疮患者中的表达变化及其临床意义[J]. 中华风湿病学杂志,2016,20(9):597-603.
[8]	罗雄燕,杨明辉,夏艳辉,等. 系统性红斑狼疮患儿外周血单个核细胞微管相关蛋白1轻链3mRNA表达水平及其意义[J]. 中华内科杂志,2015,54(2):134-138.
[9]	周士亮,徐婷,蔡明渊,等. 系统性红斑狼疮患者外周血程序性死亡受体1hi CXCR5-CD4+ T细胞的表达[J]. 中华风湿病学杂志,2019,23(1):15-18.
[10]	NUSSBAUM G,BEN-ADI S,GENZLER T,et al.Involvement of Toll-like receptors 2 and 4 in the innate immune response to Treponema denticola and its outer sheath components[J]. Infect Immun,2009,77(9):3939-3947.
[11]	URBONAVICIUTE V,FÜRNROHR B G,MEISTER S,et al. Induction of inflammatory and immune responses by HMGB1-nucleosome complexes:implications for the pathogenesis of SLE[J]. J Exp Med,2008,205(13):3007-3018.
[12]	杜灿灿,张卓亚,宋骅,等. 间充质干细胞上调系统性红斑狼疮外周血来源巨噬细胞肿瘤坏死因子-α诱导蛋白8样分子2的作用研究[J]. 中华风湿病学杂志,2019,23(3):160-164.
[13]	张玮,杨冉,郭惠芳,等. HMGB1和TNFAIP3在狼疮性肾炎系膜细胞增殖中的作用[J]. 中国药理学通报,2017,33(8):1109-1113.
[14]	叶筱燕,戴永江,蒙秉新,等. 系统性红斑狼疮患者外周血CXCL9表达水平及其临床意义[J]. 重庆医学,2015,44(5):655-656.
[15]	李雪,黄自坤,邓桢,等. 系统性红斑狼疮患儿外周血NK细胞TIGIT的表达和意义[J]. 免疫学杂志,2018,34(7):607-611.

组别	例数	IL-17/（pg/mL）	IL-23/（pg/mL）	HMGB1/（μg/mL）	TLR-4/（ng/mL）
SLE组	40	206.44±96.56	176.33±75.44	21.41±1.82	86.24±10.17
正常对照组	15	76.39±18.25	46.23±17.07	12.16±0.47	65.29±7.45
t值		5.153	6.579	19.339	7.263
P值		<0.001	<0.001	<0.001	<0.001

组别	例数	IL-17/（pg/mL）	IL-23/（pg/mL）	HMGB1/（μg/mL）	TLR-4/（ng/mL）
SLE组	40	206.44±96.56	176.33±75.44	21.41±1.82	86.24±10.17
正常对照组	15	76.39±18.25	46.23±17.07	12.16±0.47	65.29±7.45
t值		5.153	6.579	19.339	7.263
P值		<0.001	<0.001	<0.001	<0.001

组别	例数	IL-17/（pg/mL）	IL-23/（pg/mL）	HMGB1/（μg/mL）	TLR-4/（ng/mL）
活动期组	20	226.23±106.17^*	189.22±73.46^*	25.18±2.77^*	90.15±5.17^*
稳定期组	20	172.41±48.25^*#	137.03±40.15^*#	16.35±0.95^*#	84.34±7.01^*#
正常对照组	15	76.39±18.25	46.23±17.07	12.16±0.47	65.29±7.45
F值		19.160	33.429	248.600	65.580
P值		<0.001	<0.001	<0.001	<0.001

组别	例数	IL-17/（pg/mL）	IL-23/（pg/mL）	HMGB1/（μg/mL）	TLR-4/（ng/mL）
活动期组	20	226.23±106.17^*	189.22±73.46^*	25.18±2.77^*	90.15±5.17^*
稳定期组	20	172.41±48.25^*#	137.03±40.15^*#	16.35±0.95^*#	84.34±7.01^*#
正常对照组	15	76.39±18.25	46.23±17.07	12.16±0.47	65.29±7.45
F值		19.160	33.429	248.600	65.580
P值		<0.001	<0.001	<0.001	<0.001

组别	例数	IL-17/（pg/ml）	IL-23/（pg/mL）	HMGB1/（μg/mL）	TLR-4/（ng/mL）
狼疮性肾炎组	24	206.20±102.17^*#	191.31±75.06^*#	26.02±2.37^*	92.14±2.17^*#
非肾炎组	16	152.36±43.14^*	142.13±40.67^*	18.76±1.88^*	82.06±5.22^*
正常对照组	15	76.39±18.25	46.23±17.07	12.16±0.47	65.29±7.45
F值		14.850	31.960	254.740	133.720
P值		<0.001	<0.001	<0.001	<0.001