Abstract:

Objective To evaluate the role of anti-neutrophil cytoplasmic antibody（ANCA） determination for systemic vasculitis. Methods Indirect immunofluorescence（IIF） was performed to determine serum ANCA in 13 866 patients,and enzyme-linked immunosorbent assay（ELISA） was used to determine anti-myeloperoxidase（MPO） antibody and anti-protease 3（PR3） antibody. Results Among the 13 866 patients,there were 318 positive cases of ANCA,with the positive rate of 2.29%. There were 135（2.07%） males and 183（2.50%） females,and there was no statistical significance between the 2 groups（χ²=2.650,P>0.05）. The difference of the ANCA positive rate with different ages had statistical significance（χ²=28.978,P<0.01）. The positive rate of ANCA between males and females in different age groups had statistical significance（χ²=54.550,P<0.01;χ²=17.800,P<0.01）. The p-ANCA and c-ANCA and target antigen distribution had statistical significance（χ²=86.524,P<0.01）. Conclusions ANCA is potential in the diagnosis and treatment of vasculitis and autoimmune diseases,especially for ANCA-associated systemic vasculitis.

Key words: Anti-neutrophil cytoplasmic antibody, Anti-myeloperoxidase antibody, Anti-protease 3 antibody, Wegener's granulomatosis, Systemic lupus erythematosus