Diagnostic role of miRNA-21-5p for esophageal carcinoma and correlation of STAT3

doi:10.3969/j.issn.1673-8640.2020.06.004

Abstract

Abstract:

Objective To evaluate the diagnostic role of microRNA（miRNA）-21-5p for esophageal carcinoma and its mechanism. Methods A total of 78 patients with esophageal carcinoma from January 2016 to June 2018 in Xiasha Branch,Sir Run Run Shaw Hospital,Zhejiang University School of Medicine were enrolled as case group,and 69 healthy subjects were enrolled as control group. Blood and esophageal carcinoma and adjacent tissues were collected before treatment and during operation. The expression of miRNA-21-5p in serum was determined by real-time polymerase chain reaction（PCR）. The target gene of miRNA-21-5p was predicted by 3 databases,including picTar,TagetScan and Tarbase. The target gene intersection of the 3 databases was predicted using Draw venn diagram. Real-time PCR was used to determine the expression of signal transducer and activator of transcription 3（STAT3） mRNA in esophageal carcinoma and adjacent tissues in patients with esophageal carcinoma. Results The results of real-time PCR showed that miRNA-21-5p was highly expressed in serum of patients with esophageal carcinoma,which was higher than that in control group（P<0.01）. The high expression of STAT3 mRNA was also found in esophageal carcinoma tissues of patients with esophageal carcinoma,which was higher than that in adjacent tissues. Spearman correlation analysis showed that miRNA-21-5p and STAT3 were positively correlated. Conclusions The expression of miRNA-21-5p in serum of patients with esophageal carcinoma is high,and STAT3 mRNA is also highly expressed in esophageal carcinoma tissues. There is a positive correlation between miRNA-21-5p and STAT3.

Key words: MicroRNA-21-5p, Esophageal carcinoma, Diagnostic value

CLC Number:

R446.1

WANG Yanzhong, DING Limin, CHEN Yirui, WANG Yongbin. Diagnostic role of miRNA-21-5p for esophageal carcinoma and correlation of STAT3[J]. Laboratory Medicine, 2020, 35(6): 531-534.

Figures/Tables 3

References 13

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