Abstract:

Objective To investigate the distribution characteristics of human cytomegalovirus（HCMV） glycoprotein H（gH） genotyping among children in Zhejiang. Methods Urine,throat swab or mothers' milk sample was collected from children with positive HCMV DNA of Children's Hospital of Zhejiang University School of Medicine from January 2015 to December 2017. The genotyping of gH gene was performed using fluorescence quantitation polymerase chain reaction（PCR）. Children with positive HCMV DNA were classified according to ages,sample types and diseases. The genotype distribution of gH gene was compared. Results The gH genotyping was performed in 1 232 positive HCMV samples. There were 766 （62.2%） cases of gH1,373（30.3%） cases of gH2 and 93（7.5%） cases of gH1+gH2. The distribution trend of gH genotyping in positive HCMV children with different ages,different sample types and different diseases was consistent,without statistical significance （P>0.05）. Conclusions HCMV infection is mainly caused by gH1 in Zhejiang,and the distribution of HCMV gH genotypes shows no statistical significance in different ages,different sample types and different diseases.

Key words: Human cytomegalovirus, Glycoprotein H, Gene, Children