Solutions for decreasing the number of smudge cells in peripheral blood smears

doi:10.3969/j.issn.1673-8640.2017.06.017

Abstract

Abstract:

Objective To investigate the solutions for decreasing the number of smudge cells in peripheral blood smears. Methods Samples was collected in ethylene diamine tetraacetic acid-K₂ anticoagulation tubes from 20 patients with chronic lymphocytic leukemia（CLL）. Blood smears were automatically prepared with each angle and speed by hematology analyzer and automated slide maker and stainer. Smudge cells were counted by DM96 automated blood cell morphometry analyzer. After treatment with different ratios of fetal bovine serum,the results were compared. Results It is better to decrease the number of smudge cells with the speed of 140 m/s at 27 degrees. When the ratio of fetal bovine serum to peripheral blood was 1∶4,the number of smudge cells was decreased. Conclusions When a large number of smudge cells are appearing in peripheral blood smears,it should not only use an appropriate angle and speed,but also add fetal bovine serum to decrease the number of smudge cells,so as to provide an accurate classification and counting results for clinical practice.

Key words: Smudge cells, Angle, Speed, Fetal bovine serum, Chronic lymphocytic leukemia

CLC Number:

R446.1

PENG Zhenping, WANG Jianbiao, GUO Ping, SUN Fenyong. Solutions for decreasing the number of smudge cells in peripheral blood smears[J]. Laboratory Medicine, 2017, 32(6): 520-523.

Figures/Tables 5

References 13

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角度（°）	速度（m/s）	患者1	患者2	患者3	患者4	患者5
20	80	254	5	29	132	218
20	110	169	4	44	87	182
20	140	160	3	51	80	168
27	80	147	1	59	105	184
27	110	128	3	28	70	153
27	140	56	2	16	39	34
34	80	266	3	42	137	170
34	110	174	2	19	92	89
34	140	14	2	19	11	12

角度（°）	速度（m/s）	患者1	患者2	患者3	患者4	患者5
20	80	254	5	29	132	218
20	110	169	4	44	87	182
20	140	160	3	51	80	168
27	80	147	1	59	105	184
27	110	128	3	28	70	153
27	140	56	2	16	39	34
34	80	266	3	42	137	170
34	110	174	2	19	92	89
34	140	14	2	19	11	12