Abstract:

Objective To investigate the correlation of the expressions of lectin-like oxidized low-density lipoprotein receptor-1（LOX-1） and CX3C chemokine receptor 1（CX3CR1） from monocytes and proximal/middle coronary artery stenosis. Methods A total of 58 patients undergoing coronary angiography were enrolled. According to the results of coronary angiography,47 patients with at least one of main proximal or middle coronary artery stenosis ≥50% were as stenosis group,and 11 patients <50% were as non-stenosis group. Totally,26 volunteers were enrolled as control group. General information and parameters of the 3 groups were recorded. LOX-1 and CX3CR1 fluorescence intensities from monocytes in peripheral blood were determined by flow cytometry,and the severity of coronary artery lesion was evaluated by Gensini score. The expression levels of LOX-1 and CX3CR1 of single-branch lesion,double-branch lesion and multiple-branch lesion（3-branch or more） were compared. The correlation of the fluorescence intensities of LOX-1 and CX3CR1 from monocytes and Gensini score was analyzed. Multiple Logistic regression analysis and receiver operating characteristic（ROC） curve analysis were performed. Results The fluorescence intensities of LOX-1 and CX3CR1 from monocytes in peripheral blood in stenosis group were higher than those in non-stenosis group and control group（P<0.01）. There was no statistical significance in the fluorescence intensities of LOX-1 and CX3CR1 from monocytes in peripheral blood among single-branch lesion,double-branch lesion and multiple-branch lesion（P>0.05）. There was no correlation of the fluorescence intensities of LOX-1 and CX3CR1 from monocytes in peripheral blood with left ventricular ejection fraction（LVEF）,left ventricular end diastolic dimension（LVDD） and Gensini score in stenosis group（P>0.05）. Multiple Logistic regression analysis and ROC curve analysis showed that CX3CR1 was a factor of proximal/middle coronary artery stenosis with diagnosis efficiency [odds ratio（OR）=1.771,95% confidence interval（CI） 1.200-2.616]. The area under ROC curve was 0.733. Conclusions LOX-1 and CX3CR1 from monocytes may be involved in the process of proximal/middle coronary artery stenosis,without the correlation with stenosis degree. The high expression of CX3CR1 is a factor of proximal/middle coronary artery stenosis with diagnosis efficiency.

Key words: Lectin-like oxidized low-density lipoprotein receptor-1, CX3C chemokine receptor 1, Monocyte, Expression, Stenosis, Proximal/middle coronary artery