Comparison of dried blood spot samples and serum samples for HBV DNA quantification determination and drug resistance mutation

doi:10.3969/j.issn.1673-8640.2016.011.004

Abstract

Abstract:

Objective To assess the feasibility of dried blood spot（DBS） sample as an alternative to serum sample for hepatitis B virus（HBV）determination. Methods DBS samples and serum samples were prepared through extracting venous blood,and HBV DNA was determined for 100 patients with chronic hepatitis B,whose HBV virus load >1×10² copies/mL. DBS and serum sample results were compared by correlation analysis. HBV genotypes were analyzed in 73 patients of genotype B and genotype C. A total of 56 DBS and serum samples from patients with hepatitis B were collected randomly. Using an in-house nest polymerase chain reaction（PCR） for reverse transcriptase segment,the data were analyzed using geno2pheno. Results HBV DNA of DBS samples was determined in a range of 2-8 log₁₀ copies/mL. The correlation coefficient（r²） for HBV DNA between DBS samples and serum samples was 0.82（P<0.05）. The quantification of HBV DNA determination in DBS samples was 1 log₁₀ lower than that in serum samples. The coincidence rate of the 2 kinds of samples was 95.83%. For DBS and serum samples,the determination rates for drug resistance were 82.14% and 85.71%（P>0.05）. The coincidence rate of DBS and serum samples for HBV genotype was 100.00%. Conclusions DBS samples can be used in HBV DNA quantification and drug resistance determinations.

Key words: Dried blood spot, Hepatitis B virus, DNA, Drug resistance

CLC Number:

R446.61

ZHANG Min, WANG Qianying, LI Xin, WANG Gang, HU Yunwen. Comparison of dried blood spot samples and serum samples for HBV DNA quantification determination and drug resistance mutation[J]. Laboratory Medicine, 2016, 31(11): 944-947.

样本类型	HBeAg阳性（49例）			HBeAg阴性（24例）
样本类型	B型	C型	未分型	B型	C型	未分型
DBS	16	32	1	6	16	2
血清	16	32	1	6	16	2

样本类型	HBeAg阳性（49例）			HBeAg阴性（24例）
样本类型	B型	C型	未分型	B型	C型	未分型
DBS	16	32	1	6	16	2
血清	16	32	1	6	16	2

样本类型	药物敏感型	耐药位点
样本类型	药物敏感型	236T	204I	180M和204I
DBS	38	2	4	2
血清	38	2	6	2

样本类型	药物敏感型	耐药位点
样本类型	药物敏感型	236T	204I	180M和204I
DBS	38	2	4	2
血清	38	2	6	2

1	LI HM,WANG JQ,WANG R,et al.Hepatitis B virus genotypes and genome characteristics in China[J]. World J Gastroenterol,2015,21(21):6684-6697.
2	WU X,CRASK M,RAMIREZ H,et al.A simple method to elute cell-free HIV from dried blood spots improves their usefulness for monitoring therapy[J]. J Clin Virol,2015,65:38-40.
3	KARLSSON H,GUTHENBERG C,VON DBELN U,et al.Extraction of RNA from dried blood on filter papers after long-term storage[J]. Clin Chem,2003,49(6 Pt 1):979-981.
4	NELSON JA,HAWKINS JT,SCHANZ M,et al.Comparison of the Gen-Probe Aptima HIV-1 and Abbott HIV-1 qualitative assays with the Roche Amplicor HIV-1 DNA assay for early infant diagnosis using dried blood spots[J]. J Clin Virol,2014,60(4):418-421.
5	JARDI R,RODRIGUEZ-FRIAS F,BUTI M,et al.Usefulness of dried blood samples for quantification and molecular characterization of HBV-DNA[J]. Hepatology,2004,40(1):133-139.
6	VILLAR LM,DE OLIVEIRA JC,CRUZ HM,et al.Assessment of dried blood spot samples as a simple method for detection of hepatitis B virus markers[J]. J Med Virol,2011,83(9):1522-1529.

Comparison of dried blood spot samples and serum samples for HBV DNA quantification determination and drug resistance mutation

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