Establishment of reference measurement procedures for serum electrolytes based on ion chromatography

doi:10.3969/j.issn.1673-8640.2015.12.021

Abstract

Abstract:

Ion chromatography as a new type of liquid chromatography for the analysis of anions and cations was developed in 1970. Recently, this analytical technology has become one of fastest developing methods in the field of analytical chemistry. It has been widely used in industrial, biological, medical, food, clinic and environmental samples due to its advantages of rapidness, simplicity,sensitivity and selectivity. This article summarizes the research on accuracy of ion chromatography technology as a reference measurement procedure for the determination of serum electrolytes, in order to provide valuable reference to make the analytical measurement results traceable in the field of clinical chemistry.

Key words: Ion chromatography, Serum, Electrolyte, Reference measurement procedure

CLC Number:

R446.1

ZOU Bingde, ZOU Jihua, SHEN Min, ZHANG Man, WU Lishan, TU Minmin. Establishment of reference measurement procedures for serum electrolytes based on ion chromatography[J]. Laboratory Medicine, 2015, 30(12): 1250-1256.

Figures/Tables 5

References 35

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项目	ICP-MS	离子色谱法
准确度	<±1.0%^[33]	<±1.0%^[28]
精密度	<±1.0%^[33]	<±1.0%^[28]
检出限	ng/L	μg/L
样本分析能力	每个样本约5 min	每个样本的所有元素约30 min
能否同时检测	能,但参考测量程序一般不同时分离分析锂、钾、钠、钙、镁5种离子	能,同时分离分析锂、钾、钠、钙、镁5种离子
干扰
光(质)谱	少	-
基体	中等	样本处理后,基体干扰几乎没有
电离	很少	-
质量效应	高的对低的影响	-
同位素	有	-
可测元素数	锂、钾、钠、钙、镁、氯	锂、钾、钠、钙、镁、氯
样本用量	少	少
日常操作	容易	容易
方法试验开发	需专业技术	需专业技术
无人控制操作	能	能
易燃气体	无,但需要使用氩气	无,使用氮气提供压力
仪器费用	高	低
运行成本	高	低

项目	ICP-MS	离子色谱法
准确度	<±1.0%^[33]	<±1.0%^[28]
精密度	<±1.0%^[33]	<±1.0%^[28]
检出限	ng/L	μg/L
样本分析能力	每个样本约5 min	每个样本的所有元素约30 min
能否同时检测	能,但参考测量程序一般不同时分离分析锂、钾、钠、钙、镁5种离子	能,同时分离分析锂、钾、钠、钙、镁5种离子
干扰
光(质)谱	少	-
基体	中等	样本处理后,基体干扰几乎没有
电离	很少	-
质量效应	高的对低的影响	-
同位素	有	-
可测元素数	锂、钾、钠、钙、镁、氯	锂、钾、钠、钙、镁、氯
样本用量	少	少
日常操作	容易	容易
方法试验开发	需专业技术	需专业技术
无人控制操作	能	能
易燃气体	无,但需要使用氩气	无,使用氮气提供压力
仪器费用	高	低
运行成本	高	低

年份	RELA-A					RELA-B
年份	氯离子(mmol/L)	CV(%)	靶值(mmol/L)	偏移(%)		氯离子(mmol/L)	CV(%)	靶值(mmol/L)	偏移(%)
2012年	95.60	0.76	96.59	-0.17	107.04	0.67	108.60	-0.25
2013年	110.66	0.44	109.88	0.71	113.76	0.81	112.97	0.70

年份	RELA-A					RELA-B
年份	氯离子(mmol/L)	CV(%)	靶值(mmol/L)	偏移(%)		氯离子(mmol/L)	CV(%)	靶值(mmol/L)	偏移(%)
2012年	95.60	0.76	96.59	-0.17	107.04	0.67	108.60	-0.25
2013年	110.66	0.44	109.88	0.71	113.76	0.81	112.97	0.70