Study on the correlation between anti-TP antibody S/CO ratio by chemiluminescence immunoassay and positive results by confirmation assay

doi:10.3969/j.issn.1673-8640.2015.12.005

Abstract

Abstract:

Objective To investigate the correlation between anti-treponema pallidum(TP)antibody signal-to-cut off(S/CO)ratio by chemiluminescence immunoassay (CLIA) and positive results by confirmation assay.Methods A total of 208 anti-TP antibody positive samples (S/CO ratio≥1.0) were screened out by 2 CLIA systems, Abbott i2000 CLIA system (Abbott i2000)and Kemei Dongya Chemclin 600 CLIA system (Chemclin 600). The results were confirmed by western blotting (WB). Receiver operating characteristic(ROC)curve at optimal sensitivity and specificity was analyzed, and optimal S/CO threshold value was obtained by SPSS 19.0 software. The positive predictive value was calculated with every S/CO ratio interval, and the S/CO ratio of positive predictive value≥95%was obtained. Results By Abbott i2000 or Chemclin 600, 208 samples' S/CO ratio was determined.By WB, 35 cases of them were confirmed as negative, 128 cases were confirmed as positive, and 45 cases were indeterminate.The coincidence rates of WB compared with the 2 CLIA systems were 82.2% and 82.8%, respectively.When anti-TP antibody S/CO ratio of Abbott i2000 was ≥3.0, and anti-TP antibody S/CO ratio of Chemclin 600 was ≥4.0, the positive predictive value was ≥95%. Conclusions It is proposed that WB should be used if the S/CO ratio ranges are 1.0-3.0 determined by Abbott i2000 and 1.0-4.0 determined by Chemclin 600. If the S/CO ratio is above the upper limit, the samples can be reported as positive directly. It can not only reduce the confirmation assay sample number but also improve the reliability of determination report.

Key words: Anti-treponema pallidum antibody, Chemiluminescence immunoassay, Western blotting

CLC Number:

R446.61

XU Xiaofeng, JIANG Minglei, LU Renquan, ZOU Chunyuan, GONG Zhiyun, SHEN Li, GUO Lin. Study on the correlation between anti-TP antibody S/CO ratio by chemiluminescence immunoassay and positive results by confirmation assay[J]. Laboratory Medicine, 2015, 30(12): 1185-1189.

Figures/Tables 6

References 13

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Abbott i2000	Chemclin 600		合计
Abbott i2000	S/CO比值≥1.0		S/CO比值<1.0
S/CO比值≥1.0	181	12	193
S/CO比值<1.0	15	0	15
合计	196	12	208

Abbott i2000	Chemclin 600		合计
Abbott i2000	S/CO比值≥1.0		S/CO比值<1.0
S/CO比值≥1.0	181	12	193
S/CO比值<1.0	15	0	15
合计	196	12	208

抗TP抗体的 S/CO比值	Chemclin 600				Abbott i2000
	WB			阳性预测值(%)	WB			阳性预测值(%)
	-			+	不确定	-	+	不确定
0~0.99	8	1	3	11.1	9	3	3	25.0
1~1.99	17	6	26	26.1	22	9	31	29.0
2~2.99	4	6	7	60.0	3	25	7	89.3
3~3.99	5	12	0	70.6	1	24	2	96.0
4~4.99	1	20	4	95.2	0	15	1	100.0
5~5.99	0	12	2	100.0	0	12	0	100.0
6~9.99	0	6	2	100.0	0	15	1	100.0
10~19.99	0	6	0	100.0	0	7	0	100.0
20~29.99	0	24	0	100.0	0	18	0	100.0
30~39.99	0	24	1	100.0	0	0	0	-
≥40	0	11	0	100.0	0	0	0	-

抗TP抗体的 S/CO比值	Chemclin 600				Abbott i2000
	WB			阳性预测值(%)	WB			阳性预测值(%)
	-			+	不确定	-	+	不确定
0~0.99	8	1	3	11.1	9	3	3	25.0
1~1.99	17	6	26	26.1	22	9	31	29.0
2~2.99	4	6	7	60.0	3	25	7	89.3
3~3.99	5	12	0	70.6	1	24	2	96.0
4~4.99	1	20	4	95.2	0	15	1	100.0
5~5.99	0	12	2	100.0	0	12	0	100.0
6~9.99	0	6	2	100.0	0	15	1	100.0
10~19.99	0	6	0	100.0	0	7	0	100.0
20~29.99	0	24	0	100.0	0	18	0	100.0
30~39.99	0	24	1	100.0	0	0	0	-
≥40	0	11	0	100.0	0	0	0	-

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