Application of chromatography in the detection of arachidonic acid metabolites

doi:10.3969/j.issn.1673-8640.2015.08.019

Abstract

Abstract:

Arachidonic acid (AA) metabolite is a series of material produced by lipid peroxidation of (AA), and it is involved in many physiological and pathological processes. AA metabolites may be potential biomarkers for some diseases. Determining their quality and quantity accurately is significant in the occurrence, development, diagnosis and prediction of some diseases. Nearly 70 kinds of AA metabolites have been found, and they are all small molecule compounds. Conventional determination technology can't distinguish AA metabolites as their similar molecular structure. The development of chromatography and mass spectrometry provides a reliable technical method for the determination of AA metabolites. This paper gives a general review of the related advances.

Key words: Chromatography, Mass spectrometry, Arachidonic acid

CLC Number:

R446

JIANG Liansheng, TAN Longyi. Application of chromatography in the detection of arachidonic acid metabolites[J]. Laboratory Medicine, 2015, 30(8): 852-856.

Figures/Tables 1

References 30

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项目	TLC	GC-MS	LC-MS
优点	操作简单、经济快速	高敏感性、高特异性	高敏感性、高特异性、高通量
缺点	对AA代谢产物的区分能力有限, 仅适于少数物质的检测	预处理过程繁琐,某些热不稳定物质无法检测	仪器设备成本高、维护成本高
预处理	萃取后可提高检测敏感性,但非必须过程	热不稳定的代谢产物需形成甲酯或乙酯后再检测	固相萃取、液液萃取等方法
应用	用于内源性PG、TX等的生成测定	用于沸点较低或热稳定的类花生酸的检测	适于生物样本中大多数AA代谢产物的检测

项目	TLC	GC-MS	LC-MS
优点	操作简单、经济快速	高敏感性、高特异性	高敏感性、高特异性、高通量
缺点	对AA代谢产物的区分能力有限, 仅适于少数物质的检测	预处理过程繁琐,某些热不稳定物质无法检测	仪器设备成本高、维护成本高
预处理	萃取后可提高检测敏感性,但非必须过程	热不稳定的代谢产物需形成甲酯或乙酯后再检测	固相萃取、液液萃取等方法
应用	用于内源性PG、TX等的生成测定	用于沸点较低或热稳定的类花生酸的检测	适于生物样本中大多数AA代谢产物的检测