The influence of beta-glucan on TNF-α,IL-8 and IL-12 in monocytes

doi:10.3969/j.issn.1673-8640.2015.03.019

Abstract

Abstract: Objective

To study the influence of beta-glucan on tumor necrosis factor alpha (TNF-α), interleukin 8 (IL-8) and interleukin 12 (IL-12) in atherosclerosis pathogenesis monocytes.

Methods

The monocytes of healthy subjects were separated by density gradient centrifugation. The monocytes were induced into macrophages by phorbol myristate acetate (PMA), and the macrophages were induced into foam cells by oxidized low-density lipoprotein (ox-LDL). There were control group, ox-LDL group, whole beta-glucan particle (WGP) group and WGP + ox-LDL group, the total RNA was extracted, and the TNF-α, IL-8 and IL-12 cDNA was amplified. The concentrations in supernates were determined by enzyme-linked immunosorbent assay(ELISA).

Results

Compared with control group,the protein expression of TNF-α ,IL-8 and IL-12 in monocytes were significantly up-regulated by ox-LDL (P<0.05). After pretreated with WGP,the protein expressions of TNF-α, IL-8 and IL-12 and the mRNA expressions of TNF-α and IL-8 were inhibited significantly (P<0.05).

Conclusions

beta-glucan may inhibited ox-LDL-induced pro-inflammatory effects in macrophages,which attenuates the development of atherosclerosis.

Key words: Beta-glucan, Low-density lipoprotein, Monocytes

CLC Number:

R446.62

WU Rongqiang, WANG Sujian, WANG Shizhong, ZHOU Haijun, QI Chunjian. The influence of beta-glucan on TNF-α,IL-8 and IL-12 in monocytes[J]. Laboratory Medicine, 2015, 30(3): 280-283.

Figures/Tables 4

References 11

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引物名称	序列(5'→3')
TNF-α正义引物	GCCAGAGGGCTGATTAGAGA
TNF-α反义引物	TCAGCCTCTTCTCCTTCCTG
IL-8 正义引物	AAATTTGGGGTGGAAAGGTT
IL-8 反义引物	TCCTGATTTCTGCAGCTCTGT
IL-12 正义引物	CCTCCACTGTGCTGGTTTTAT
IL-12 反义引物	TCAGCAACATGCTCCAGAAG
GAPDH正义引物	GGAAGGTGAAGGTCGGAGTC
GAPDH反义引物	CGTTCTCAGCCTTGACGGT

引物名称	序列(5'→3')
TNF-α正义引物	GCCAGAGGGCTGATTAGAGA
TNF-α反义引物	TCAGCCTCTTCTCCTTCCTG
IL-8 正义引物	AAATTTGGGGTGGAAAGGTT
IL-8 反义引物	TCCTGATTTCTGCAGCTCTGT
IL-12 正义引物	CCTCCACTGTGCTGGTTTTAT
IL-12 反义引物	TCAGCAACATGCTCCAGAAG
GAPDH正义引物	GGAAGGTGAAGGTCGGAGTC
GAPDH反义引物	CGTTCTCAGCCTTGACGGT

组别	TNF-α	IL-8	IL-12
无药对照组	13.00±1.73	500.00±96.15	115.00±10.39
ox-LDL组	38.00±5.57^*	940.70±91.43^*	240.70±23.69^*
WGP+ox-LDL组	23.33±4.16^#	419.00±58.80^#	106.30±15.82^#
WGP组	15.33±1.53	208.30±43.66	99.33±14.05

组别	TNF-α	IL-8	IL-12
无药对照组	13.00±1.73	500.00±96.15	115.00±10.39
ox-LDL组	38.00±5.57^*	940.70±91.43^*	240.70±23.69^*
WGP+ox-LDL组	23.33±4.16^#	419.00±58.80^#	106.30±15.82^#
WGP组	15.33±1.53	208.30±43.66	99.33±14.05

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