Rapid identification of Yeast like fungi outside of Candida albicans isolated form blood culture by two MALDI-TOF MS systems

doi:10.3969/j.issn.1673-8640.2015.02.006

Abstract

Abstract: Objective

To evaluate the application of 2 matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), Autoflex MALDI-TOF MS and Vitek MALDI-TOF MS, for the identification of Yeast like fungi outside of Candida albicans isolated from blood culture.

Methods

A total of 59 isolates were collected from blood culture from October 2010 to February 2013 among inpatients in Zhongshan Hospital, Fudan University. The results of fungus internal transcribed spacer (ITS) sequencing were as golden standards. The correct identification rates of Autoflex MALDI-TOF MS, Vitek MALDI-TOF MS and API 20C AUX were analyzed comparatively.

Results

The 59 isolates included 11 types of fungi, namely, Candida parapsilosis complex(30 isolates, 50.8%), Candida tropicalis(11 isolates, 18.6%), Candida glabrata(8 isolates, 13.6%), Candida krusei( 2 isolates, 3.4%), Wickerhamomy cesanomalus(2 isolates, 3.4%), Cryptococcus neoformans(2 isolates, 3.4%), Candida iusitaniae(1 isolate, 1.7%), Candida guilliermondii (1 isolate, 1.7%), Trichosporon asahii(1 isolate, 1.7%) and Saccharomyces cerevisiae (1 isolate, 1.7%). The correct identification rates by Autoflex MALDI-TOF MS, Vitek MALDI-TOF MS and API 20C AUX were 93.2%, 83.1% and 79.7%, respectively, and there was no statistical significance between the 2 MALDI-TOF MS systems (P=0.155). The correct identification rates for yeast like fungi outside of Candida albicans by Autoflex MALDI-TOF MS, Vitek MALDI-TOF MS and API 20C AUX were 100.0%, 84.9% and 84.9%, respectively, and there was statistical significance between the 2 MALDI-TOF MS systems(P=0.006). The correct identification rates of rarely clinical encountered fungi by Autoflex MALDI-TOF MS, Vitek MALDI-TOF MS and API 20C AUX were 33.3%, 66.6% and 33.3%, respectively.

Conclusions

MALDI-TOF MS system is rapid, simple and accurate compared with current routine biochemical identification methods. The performance of Autoflex MALDI-TOF MS is similar with that of Vitek MALDI-TOF MS for the identification of isolated fungi from blood culture. The performance of Autoflex MALDI-TOF MS is better than that of Vitek MALDI-TOF MS for the identification of yeast like fungi outside of Candida albicans.

Key words: Matrix-assisted laser desorption/ionization-time of flight mass spectrometry, Non-Candida albicans, Blood culture

CLC Number:

R446.5

HUANG Shenglei, HU Bijie, CHEN Rong, ZHOU Chunmei. Rapid identification of Yeast like fungi outside of Candida albicans isolated form blood culture by two MALDI-TOF MS systems[J]. Laboratory Medicine, 2015, 30(2): 128-131.

Figures/Tables 2

References 7

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ITS序列鉴定细菌	例数	Autoflex MALDI-TOF MS		Vitek MALDI-TOF MS		API 20C AUX
ITS序列鉴定细菌	例数	正确(%)	错误(%)	正确(%)	错误(%)	正确(%)	错误(%)
近平滑念珠菌	22	22(100.0)	0(0.0)	22(100.0)	0(0.0)	22(100.0)	0(0.0)
热带念珠菌	11	11(100.0)	0(0.0)	11(100.0)	0(0.0)	11(100.0)	0(0.0)
光滑念珠菌	8	8(100.0)	0(0.0)	8(100.0)	0(0.0)	8(100.0)	0(0.0)
Candida metapsilosis	8	8(100.0)	0(0.0)	0(0.0)	8(100.0)	0(0.0)	8(100.0)
克柔念珠菌	2	2(100.0)	0(0.0)	2(100.0)	0(0.0)	2(100.0)	0(0.0)
新生隐球菌	2	2(100.0)	0(0.0)	2(100.0)	0(0.0)	2(100.0)	0(0.0)
异常威克汉姆酵母	2	0(0.0)	2(100.0)	0(0.0)	2(100.0)	0(0.0)	2(100.0)
季也蒙念珠菌	1	1(100.0)	0(0.0)	1(100.0)	0(0.0)	1(100.0)	0(0.0)
葡萄牙念珠菌	1	1(100.0)	0(0.0)	1(100.0)	0(0.0)	1(100.0)	0(0.0)
阿萨希毛孢子菌	1	0(0.0)	1(100.0)	1(100.0)	0(0.0)	0(0.0)	1(100.0)
酿酒酵母	1	0(0.0)	1(100.0)	1(100.0)	0(0.0)	0(0.0)	1(100.0)

ITS序列鉴定细菌	例数	Autoflex MALDI-TOF MS		Vitek MALDI-TOF MS		API 20C AUX
ITS序列鉴定细菌	例数	正确(%)	错误(%)	正确(%)	错误(%)	正确(%)	错误(%)
近平滑念珠菌	22	22(100.0)	0(0.0)	22(100.0)	0(0.0)	22(100.0)	0(0.0)
热带念珠菌	11	11(100.0)	0(0.0)	11(100.0)	0(0.0)	11(100.0)	0(0.0)
光滑念珠菌	8	8(100.0)	0(0.0)	8(100.0)	0(0.0)	8(100.0)	0(0.0)
Candida metapsilosis	8	8(100.0)	0(0.0)	0(0.0)	8(100.0)	0(0.0)	8(100.0)
克柔念珠菌	2	2(100.0)	0(0.0)	2(100.0)	0(0.0)	2(100.0)	0(0.0)
新生隐球菌	2	2(100.0)	0(0.0)	2(100.0)	0(0.0)	2(100.0)	0(0.0)
异常威克汉姆酵母	2	0(0.0)	2(100.0)	0(0.0)	2(100.0)	0(0.0)	2(100.0)
季也蒙念珠菌	1	1(100.0)	0(0.0)	1(100.0)	0(0.0)	1(100.0)	0(0.0)
葡萄牙念珠菌	1	1(100.0)	0(0.0)	1(100.0)	0(0.0)	1(100.0)	0(0.0)
阿萨希毛孢子菌	1	0(0.0)	1(100.0)	1(100.0)	0(0.0)	0(0.0)	1(100.0)
酿酒酵母	1	0(0.0)	1(100.0)	1(100.0)	0(0.0)	0(0.0)	1(100.0)

ITS序列鉴定	菌株数	Autoflex MALDI-TOF MS(鉴定值)	MALDI-TOF MS	API 20C AUX
Candida metapsilosis	8	Candida metapsilosis(2.092~2.243)	近平滑念珠菌	近平滑念珠菌
异常威克汉姆酵母菌	2	结语膜醭假丝酵母菌(1.882,1.986)	结语膜醭假丝酵母菌	无结果
阿萨希毛孢子菌	1	白念珠菌(1.301)	阿萨希毛孢子菌	无结果
酿酒酵母菌	1	Candida robusta(1.611)	酿酒酵母菌	无结果

ITS序列鉴定	菌株数	Autoflex MALDI-TOF MS(鉴定值)	MALDI-TOF MS	API 20C AUX
Candida metapsilosis	8	Candida metapsilosis(2.092~2.243)	近平滑念珠菌	近平滑念珠菌
异常威克汉姆酵母菌	2	结语膜醭假丝酵母菌(1.882,1.986)	结语膜醭假丝酵母菌	无结果
阿萨希毛孢子菌	1	白念珠菌(1.301)	阿萨希毛孢子菌	无结果
酿酒酵母菌	1	Candida robusta(1.611)	酿酒酵母菌	无结果

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