Application of MALDI-TOF MS in direct identification of clinical microbiological samples

doi:10.3969/j.issn.1673-8640.2015.07.019

Abstract

Abstract:

Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS),as a newly developed technology in recent years,is an easy,rapid,accurate and cost-effective method for microbial identification in clinical microbiology, compared to conventional phenotypic techniques or molecular biology. Given the accuracy and reliability of MALDI-TOF MS in the identification of microorganisms grown on solid medium,this technology might also be directly applied to some clinical microbiological samples, such as positive blood culture bottles,midstream urine, cerebrospinal fluid and so on. The goal of this review was to discuss the application of MALDI-TOF MS technology in direct identification of clinical microbiological samples, including the principles, protocols, performance and research progress.

Key words: Matrix-assisted laser desorption/ionization-time of flight mass spectrometry, Direct identification, Microbial identification, Clinical sample

CLC Number:

R446.5

CHEN Fei, HU Binjie, ZHAO Hu. Application of MALDI-TOF MS in direct identification of clinical microbiological samples[J]. Laboratory Medicine, 2015, 30(7): 750-756.

References 38

[1]	ANHALT JP.Identification of bacteria using mass spectrometry[J]. Analytical Chemistry,1975,47(2):219-225.
[2]	CROXATTO A,PROD'HOM G,GREUB G. Applications of MALDI-TOF mass spectrometry in clinical diagnostic microbiology[J]. FEMS Microbiol Rev,2012,36(2):380-407.
[3]	PATEL R.MALDI-TOF mass spectrometry: transformative proteomics for clinical microbiology[J]. Clin Chem,2013,59(2):340-342.
[4]	WANG MC,LIN WH,YAN JJ,et al.Early identification of microorganisms in blood culture prior to the detection of a positive signal in the BACTEC FX system using matrix-assisted laser desorption/ionization-time of flight mass spectrometry[J]. J Microbiol Immunol Infect,2013.[Epub ahead of print]
[5]	NONNEMANN B,TVEDE M,BJARNSHOLT T.Identification of pathogenic microorganisms directly from positive blood vials by matrix-assisted laser desorption/ionization time of flight mass spectrometry[J]. APMIS,2013,121(9):871-877.
[6]	HAMMARSTRÖM B,NILSON B,LAURELL T,et al. Acoustic trapping for bacteria identification in positive blood cultures with MALDI-TOF MS[J]. Anal Chem,2014, 86(21): 10560-10567.
[7]	FERREIRA L,SÁNCHEZ-JUANES F,GONZÁLEZ-AVILA M,et al. Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry[J]. J Clin Microbiol,2010,48(6):2110-2115.
[8]	KÖHLING HL,BITTNER A,MÜLLER KD,et al. Direct identification of bacteria in urine samples by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and relevance of defensins as interfering factors[J]. J Med Microbiol,2012,61(Pt 3):339-344.
[9]	BADER O.MALDI-TOF-MS-based species identification and typing approaches in medical mycology[J]. Proteomics,2013,13(5):788-799.
[10]	STEVENSON LG,DRAKE SK,MURRAY PR.Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry[J]. J Clin Microbiol,2010,48(2):444-447.
[11]	LA SCOLA B,RAOULT D.Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry[J]. PLoS One,2009,4(11):e8041.
[12]	MARCH ROSSELLÓ GA, GUTIÉRREZ RODRÍGUEZ MP, ORTIZ DE LEJARAZU LEONARDO R,et al. New procedure for rapid identification of microorganisms causing urinary tract infection from urine samples by mass spectrometry (MALDI-TOF)[J]. Enferm Infecc Microbiol Clin,2015, 33(2): 89-94.
[13]	GORTON RL,RAMNARAIN P,BARKER K,et al.Comparative analysis of Gram's stain,PNA-FISH and Sepsityper with MALDI-TOF MS for the identification of yeast direct from positive blood cultures[J]. Mycoses,2014,57(10):592-601.
[14]	VLEK AL,BONTEN MJ,BOEL CH.Direct matrix-assisted laser desorption ionization time-of-flight mass spectrometry improves appropriateness of antibiotic treatment of bacteremia[J]. PLoS One,2012,7(3):e32589.
[15]	CLERC O,PROD'HOM G,VOGNE C,et al. Impact of matrix-assisted laser desorption ionization time-of-flight mass spectrometry on the clinical management of patients with Gram-negative bacteremia: a prospective observational study[J]. Clin Infect Dis,2013,56(8):1101-1107.
[16]	YAN Y,HE Y,MAIER T,et al.Improved identification of yeast species directly from positive blood culture media by combining Sepsityper specimen processing and Microflex analysis with the matrix-assisted laser desorption ionization Biotyper system[J]. J Clin Microbiol,2011,49(7):2528-2532.
[17]	PROD'HOM G,BIZZINI A,DURUSSEL C,et al. Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets[J]. J Clin Microbiol,2010,48(4):1481-1483.
[18]	FERRONI A,SUAREZ S,BERETTI JL,et al.Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry[J]. J Clin Microbiol,2010,48(5):1542-1548.
[19]	SCHMIDT V,JAROSCH A,MÄRZ P,et al. Rapid identification of bacteria in positive blood culture by matrix-assisted laser desorption ionization time-of-flight mass spectrometry[J]. Eur J Clin Microbiol Infect Dis,2012,31(3):311-317.
[20]	SCHUBERT S,WEINERT K,WAGNER C,et al.Novel,improved sample preparation for rapid,direct identification from positive blood cultures using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry[J]. J Mol Diagn,2011,13(6):701-706.
[21]	陈峰,李媛睿,皇甫昱婵,等.分离胶促凝管联合MALDI-TOF MS直接检测血培养阳性细菌[J].检验医学,2015,30(2):113-121.
[22]	PAOLUCCI M,FOSCHI C,TAMBURINI MV,et al.Comparison between MALDI-TOF MS and FilmArray Blood Culture Identification panel for rapid identification of yeast from positive blood culture[J]. J Microbiol Methods,2014,104:92-93.
[23]	KROUMOVA V,GOBBATO E,BASSO E,et al.Direct identification of bacteria in blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: a new methodological approach[J]. Rapid Commun Mass Spectrom,2011,25(15):2247-2249.
[24]	IDELEVICH EA,SCHÜLE I,GRÜNASTEL B,et al. Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium[J]. Clin Microbiol Infect,2014, 20(10):1001-1006.
[25]	HONG SK,CHANG BK, SONG SH,et al.Use of MALDI-TOF MS technique for rapid identification of bacteria from positive blood cultures[J]. Indian J Med Microbiol,2014,32(4):419-422.
[26]	MACHEN A,DRAKE T,WANG YF.Same day identification and full panel antimicrobial susceptibility testing of bacteria from positive blood culture bottles made possible by a combined lysis-filtration method with MALDI-TOF VITEK mass spectrometry and the VITEK2 system[J]. PLoS One,2014,9(2):e87870.
[27]	PEREZ KK,OLSEN RJ,MUSICK WL,et al.Integrating rapid pathogen identification and antimicrobial stewardship significantly decreases hospital costs[J]. Arch Pathol Lab Med,2013,137(9):1247-1254.
[28]	SZABADOS F,MICHELS M,KAASE M,et al.The sensitivity of direct identification from positive BacT/ALERTTM (bioMérieux) blood culture bottles by matrix-assisted laser desorption ionization time-of-flight mass spectrometry is low[J]. Clin Microbiol Infect,2011,17(2):192-195.
[29]	ROMERO-GÓMEZ M P,MINGORANCE J. The effect of the blood culture bottle type in the rate of direct identification from positive cultures by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry[J]. J Infect,2011,62(3):251-253.
[30]	SÁNCHEZ-JUANES F,SILLER RUIZ M,MORENO OBREGON F,et al. Pretreatment of urine samples with SDS improves direct identification of urinary tract pathogens with matrix-assisted laser desorption ionization-time of flight mass spectrometry[J]. J Clin Microbiol,2013,52(1):335-338.
[31]	DEMARCO ML,BURNHAM CA.Diafiltration MALDI-TOF mass spectrometry method for culture-independent detection and identification of pathogens directly from urine specimens[J]. Am J Clin Pathol,2014,141(2):204-212.
[32]	LEE TF,LEE H,CHEN CM,et al.Comparison of the accuracy of matrix-assisted laser desorption ionization-time of flight mass spectrometry with that of other commercial identification systems for identifying Staphylococcus saprophyticus in urine[J]. J Clin Microbiol,2013,51(5):1563-1566.
[33]	杨溪,王新华,隋文君,等. MALDI-TOF MS直接鉴定原始尿液中的病原菌[J]. 中华检验医学杂志,2013,36(11):1042-1044.
[34]	MARCH ROSSELLÓ GA,GUTIÉRREZ RODRÍGUEZ MP,DE LEJARAZU LEONARDO RO,et al. Procedure for microbial identification based on matrix-assisted laser desorption/ionization-time of flight mass spectrometry from screening-positive urine samples[J]. APMIS,2014,122(9):790-795.
[35]	WANG XH,ZHANG G,FAN YY,et al.Direct identification of bacteria causing urinary tract infections by combining matrix-assisted laser desorption ionization-time of flight mass spectrometry with UF-1000i urine flow cytometry[J]. J Microbiol Methods,2013,92(3):231-235.
[36]	王琳,李佳萍,陈功祥,等. MALDI-TOF MS在中段尿样本细菌直接检测中的应用[J].检验医学,2015,30(2): 108-112.
[37]	NYVANG HARTMEYER G,KVISTHOLM JENSEN A,BÖCHER S,et al. Mass spectrometry: pneumococcal meningitis verified and Brucella species identified in less than half an hour[J]. Scand J Infect Dis,2010,42(9):716-718.
[38]	SEGAWA S,SAWAI S,MURATA S,et al.Direct application of MALDI-TOF mass spectrometry to cerebrospinal fluid for rapid pathogen identification in a patient with bacterial meningitis[J]. Clin Chim Acta,2014,435:59-61.