Abstract:

Objective　To testify the significance of comprehensive analysis in the diagnosis of leukemia. Methods　A total of 6 cases of leukemia， including acute promyelocytic leukemia M3a， large granular lymphocyte leukemia， hairy cell leukemia（HCL）， acute myeloblastic leukemia with significant maturation M2a， chronic lymphocyte leukemia（CLL） and acute myeloblastic leukemia without cytologic maturation M0 were diagnosed by routine blood testing， bone marrow morphology， cellular chemical dyeing， flow cytometry， cellular genetics， molecular biology analysis and bone marrow pathological diagnosis. Results　Acute promyelocytic leukemia was confirmed through bone marrow morphology and cellular chemical dyeing. Large granular lymphocyte leukemia was confirmed through bone marrow morphology，cellular chemical dyeing and flow cytometry. HCL was confirmed through bone marrow morphology， cellular chemical dyeing， flow cytometry and bone marrow pathological diagnosis. However， the diagnosis of acute myeloblastic leukemia with significant maturation manifested that it was still hard to make the decision through bone marrow morphology， cellular chemical dyeing and flow cytometry. The confirmation was made in aid of chromosome examination and molecular biology analysis. CLL was confirmed through bone marrow morphology and flow cytometry. Acute myeloblastic leukemia without cytologic maturation was confirmed through bone marrow morphology， cellular chemical dyeing， flow cytometry and chromosome examination. Conclusions　The comprehensive analysis of multiple laboratory items makes critical clinical importance in the confirmation of untypical leukemia as the results of environmental changes，aging of population and so on.

Key words: Leukemia, Cellular morphology, Flow cytometry, Cellular genetics, Molecular biology