The detections of peripheral blood Th17 cells and Treg cells in HBV infected patients and their significance

doi:10.3969/j.issn.1673-8640.2014.01.005

Abstract

Abstract:

Objective To investigate the percentage changes of T helper 17 (Th17) cells and regulatory T (Treg) cells in the peripheral blood of hepatitis B virus(HBV) infected patients and their correlations with liver injury after HBV infection. Methods The peripheral blood of 34 asymptomatic HBV carrier(ASC), 50 chronic hepatitis B(CHB) patients and 32 hepatocellular carcinoma(HCC) patients were collected. A total of 30 healthy controls(control group) were enrolled. The percentages of Th17 and Treg cells were detected by flow cytometry. Alanine aminotransferase (ALT) and aspartate aminotransferase(AST) were detected by Bayer ADVIA 1650 automatic biochemistry analyzer. Results Compared with control group (1.13%±0.37%), the percentage of Th17 cells in the peripheral blood of ASC group (1.30%±0.28%) was higher with no statistical significance (P> 0.05), but it obviously increased in CHB group (2.06%±1.15%) and HCC group (5.89%±3.31%, P<0.01). The percentage of Th17 cells in HCC group was higher than that in CHB group (P<0.05). The percentage of Th17 cells was positively correlated with ALT and AST [coefficients of correlation(r) were 0.682 and 0.637, P<0.01]. Compared with control group (7.18%±2.28%), the percentage of CD4⁺CD25⁺CD127^lowTreg cells in the peripheral blood of ASC group (7.72%±2.05%) was higher with statistical significance (P> 0.05), but it increased obviously in CHB group (9.01%±2.92%) and HCC group (10.86%±1.77%, P<0.01), and that in HCC group was higher than that in CHB group(P<0.05). The correlation of Treg cells was positive with ALT and AST (r=0.452, P<0.01; r=0.307, P<0.05). The correlation of Th17 cell percentage was positive with Treg cell percentage (r=0.327, P<0.01). The rate of Th17/Treg in control group was the lowest (0.17±0.16), that in ASC group(0.18±0.13) increased slightly, and there was no statistical significance between control group and ASC group (P> 0.05). However, there was obviously increasing in CHB group (0.23±0.19, P<0.05) and HCC group (0.52±0.54, P<0.01). Results In HBV infected patients, the percentages of Th17 and Treg cells increase. As for the immune balance of Th17 and Treg cells is broken, the increasing of Th17 cells mediates immune injury and induces the inflammation of liver cells.

Key words: T helper 17 cell, Regulatory T cell, Hepatitis B virus

CLC Number:

R446.63

HU Yueming, MA Li, LI Hong.. The detections of peripheral blood Th17 cells and Treg cells in HBV infected patients and their significance[J]. , 2014, 29(1): 21-25.

References

[1] Awasthi A, Kuchroo VK. Th17 cells: from precursors to players in inflammation and infection [J]. Int Immunol, 2009, 21(5): 489-498.
[2] Peng G, Li S, Wu W, et al. Circulating CD4+CD25+regulatory T cells correlate with chronic hepatitis B infection[J]. Immunology, 2008, 123(1): 57-65.
[3] 葛婷, 唐志鹏, 王亮, 等.Th17/Treg失衡与炎症性肠病的关系[J]. 世界华人消化杂志, 2010, 18(7):689-694.
[4] 林松, 伍伟锋. Th17细胞及其在心肌炎中的作用[J]. 国际心血管病杂志, 2009, 36(3):154-156.
[5] Liu X, Yang P, Lin X, et al.Inhibitory effect of cyclosporin A and corticosteroids on the production of IFN-gamma and IL-17 by T cells in Vogt-Koyanagi-Harada syndrome [J].Clin Immunol, 2009, 131(2): 333-342.
[6] 中华医学会肝病学分会, 中华医学会感染病学分会. 慢性乙型肝炎防治指南(2010年版)[J]. 中华肝脏病杂志, 2011, 19(1):13-24.
[7] Korn T, Bettelli E, Oukka M, et al. IL-17 and Th17 cells [J]. Annu Rev Immunol, 2009, 27: 485-517.
[8] 郑源海, 周艳贞, 林元峰, 等.慢性乙型肝炎患者外周血T、B淋巴细胞变化与其HBV- DNA的水平相关性探讨[J]. 实验与检验医学, 2010, 28(6):584-585.
[9] Stoop JN, van der Molen RG, Kuipers EJ, et al.Inhibition of viral replication reduces regulatory T cells and enhances the antiviral immune response in chronic hepatitis B[J].Virology, 2007, 361(1): 141-148.
[10] 郑峻松, 吴军, 易绍萱, 等. CD4+CD25+Treg细胞调节T细胞的作用机制分析[J]. 第三军医大学学报, 2003, 25(21):1881-1884.
[11] 朱杰, 杜文波, 高润平, 等. 慢性乙型肝病患者肝组织和血清TGF-β1的测定及临床意义[J]. 中国实验诊断学, 2010, 14(7):1073-1075.
[12] 邰伯军, 姚登福, 邱历伟, 等. 肝癌组织TGF-β1和IGF-Ⅱ表达水平与HBV复制关系[J]. 现代医药卫生, 2007, 23(18):2691-2693.

[1]	ZHANG Ying, JING Lijuan, LI Li. Relationship between the expression of miR-138 in peripheral blood mononuclear cells of hepatitis B virus proliferation in carriers [J]. Laboratory Medicine, 2023, 38(6): 559-562.
[2]	LIU Yang, GENG Kunjing, LI Hongjiang, SHI Haoxi, CHEN Sisi. Relationship of 25（OH）D₃ and liver inflammatory stage with the efficacy of antiviral therapy [J]. Laboratory Medicine, 2022, 37(7): 628-631.
[3]	MA Yanting, SU Xi, HUANG Fei, WANG Mengxian, SHEN Minna, ZHANG Chunyan, WANG Beili, PAN Baishen, GUO Wei. Clinical role of HBV RNA detection in chronic hepatitis B [J]. Laboratory Medicine, 2022, 37(1): 72-77.
[4]	LI Caidong, ZHANG Xuqiang, LEI Zhiping, CHEN Qiaoli, CHEN Lu, TIAN Pengfei, DUAN Zhengjun. Clinical values of IL-1β,IL-6 and TNF-α in different clinical courses of chronic HBV infection [J]. Laboratory Medicine, 2021, 36(8): 790-794.
[5]	LIU Tingting, LIN Yuting, Mila , LI Xiaoqin. Correlation between serum IL-33 level and its gene polymorphism and the clinical outcome of HBV infection [J]. Laboratory Medicine, 2021, 36(11): 1101-1105.
[6]	SONG Hui, YANG Lan, XU Limin, SHEN Zhenhua, LIU Qianqian, LIU Xinghui. Influence and mechanism of hepatitis B virus on the expression of HOXA10 [J]. Laboratory Medicine, 2021, 36(11): 1172-1176.
[7]	YUAN Yongming, ZHANG Xiaoying, GU Chao, ZHANG Jue, SUN Xuehua. Analysis of hepatitis B virus S protein mutation in chronic hepatitis B and cirrhosis patients [J]. Laboratory Medicine, 2020, 35(5): 428-433.
[8]	LIU Yang, ZHANG Zhiping, PENG Daorong, SONG Liuwei, GE Shengxiang, HAO Xiaoke, LIU Jiayun. Establishment and performance evaluation of chemiluminescent microparticle immunoassay for the determination of high concentration HbsAg [J]. Laboratory Medicine, 2020, 35(10): 1056-1061.
[9]	ZOU Guanghui, SUN Fenyong, DAI Jiaqi, YAN Jie, ZHANG Yun, YIN Zhiqiang. Role of follicular regulatory T cells in the pathogenesis of Hashimoto thyroiditis [J]. Laboratory Medicine, 2019, 34(8): 682-686.
[10]	ZHAO Lian, HE Jinlong, WU Cuiyun, ZHOU Haiyan, ZHOU Weilun, WU Cuiping. Role of peripheral blood CD4⁺CD25^highTreg percentage in the prognosis of ALL [J]. Laboratory Medicine, 2019, 34(4): 327-330.
[11]	KE Xing, ZHANG Liang, SHEN Lisong. Preliminary study on the mechanism of CD4⁺CD25^highCD127^low regulatory T cells promoting the metastasis of epithelial ovarian cancer [J]. Laboratory Medicine, 2019, 34(2): 110-115.
[12]	JIANG Lingli, XIAO Yanqun, WANG Xueliang, BAO Yun, YANG Yixiao, WANG Hualiang. Development and application of external quality assessment research material for YMDD mutation detection of hepatitis B virus [J]. Laboratory Medicine, 2019, 34(2): 176-179.
[13]	GAO Wanqin, ZHANG Qin, ZHU Lin, WU Bin, HE Wentao, ZHANG Tinglu, ZHAO Changxin. Analysis of genotyping and 6 nucleosides' resistance of HBV in patients with chronic hepatitis B [J]. Laboratory Medicine, 2019, 34(11): 994-997.
[14]	ZHU Jun, ZHU Yuqing, ZHU Lingfeng, JIANG Lingli, XU Chong. Flow fluorescent dot matrix technique for hepatitis B virus genotyping [J]. Laboratory Medicine, 2018, 33(5): 437-441.
[15]	HE Chengshan, MA Chenyun, LU Zhicheng. Influence of hepatitis B virus Pre-S/S region gene mutation on hepatitis B surface antigen determination [J]. Laboratory Medicine, 2018, 33(10): 949-953.