Study on the determination of pregnancy associated plasma protein A by ELISA

doi:10.3969/j.issn.1673-8640.2013.10.011

Abstract

Abstract:

Objective To establish a enzyme-linked immunosorbent assay (ELISA) for the diagnosis method of pregnancy associated plasma protein(PAPP-A). Methods Sera from full-term pregnant women were purified by molecular sieve and ion exchange chromatography,and then the biotin labeling PAPP-A and enzyme-labelled PAPP-A antibody were prepared. Traditional ELISA was used to detect the samples,and the sensitivity,specificity,recovery and stability were observed. The results of 30 samples from first trimester pregnant women were kit from DRG of USA. Results The result of the purification by molecular sieve and ion exchange chromatography was good. The range of standard curve was 1-50 μg/mL. The sensitivity was 0.35 μg/mL,the recovery was>100%,and the stability and specificity were good. The method had good correlation with the kit from DRG of USA,and the regression equation was Y=2.286 1+1.026 9X(r=0.982, P<0.01). Conclusions Biotin labeling PAPP-A is made, and the connection ability of biotin and avidin can be reflected in the ELISA. The ELISA is meaningful in the determination of PAPP-A.

Key words: Pregnancy associated plasma protein A, Enzyme-linked immunosorbent assay, Methodology, Biotin, Avidin

CLC Number:

R446.61

JIN Baoying,ZENG Zhaowei,LI Huiqiang.. Study on the determination of pregnancy associated plasma protein A by ELISA[J]. , 2013, 28(10): 908-912.

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