CRISPR/Cas13系统用于病原体检测的研究进展

doi:10.3969/j.issn.1673-8640.2023.08.018

检验医学 ›› 2023, Vol. 38 ›› Issue (8): 800-804.DOI: 10.3969/j.issn.1673-8640.2023.08.018

CRISPR/Cas13系统用于病原体检测的研究进展

赵亚楠, 曹啟新(), 闫彦, 李晓聪, 赵建平, 肖伟利

内蒙古自治区人民医院检验科，内蒙古呼和浩特 010010

收稿日期:2022-05-24 修回日期:2022-12-01 出版日期:2023-08-30 发布日期:2023-10-30
通讯作者: 曹啟新，E-mail：872177018@qq.com。
作者简介:赵亚楠，女，1993年生，学士，主要从事微生物分子诊断相关工作。
基金资助:
内蒙古自治区卫生健康科技计划项目(202202008)

Research progress of CRISPR/Cas13 system for pathogen detection

ZHAO Yanan, CAO Qixin(), YAN Yan, LI Xiaocong, ZHAO Jianping, XIAO Weili

Department of Clinical Laboratory，Inner Mongolia Autonomous Region People's Hospital，Hohhot 010010，Inner Mongolia，China

Received:2022-05-24 Revised:2022-12-01 Online:2023-08-30 Published:2023-10-30

摘要/Abstract

摘要：

环境和食品中的病原体严重威胁着人类生命健康，快速、准确的检测方法是高效防控病原的关键。目前，成簇规律间隔短回文重复序列（CRISPR）/成簇规律间隔短回文重复序列相关蛋白（Cas）13系统因其“附带剪切”的特性被广泛应用于病原体的检测，并被开发为一种灵敏度高、特异性强、成本低、快速便捷的新型核酸检测工具，开创了分子诊断的新时代。文章对CRISPR/Cas13系统的特点和分子机制进行介绍，综述CRISPR/Cas13技术在病原体检测中的研究进展，并对CRISPR/Cas13系统的未来发展和应用前景进行展望。

关键词: 成簇规律间隔短回文重复序列, 成簇规律间隔短回文重复序列相关蛋白, 病原体

Abstract:

Pathogen in environment and food is a serious threat to human life and health. Rapid and accurate detection methods are the keys to the effective prevention and control of the epidemic. At present，clustered regularly interspaced short palindromic repeats（CRISPR）/clustered regularly interspaced short palindromic repeat-associated protein（Cas）13 system has been widely used in the detection of pathogens due to its "attached shear" property. It has been developed as a novel nucleic acid detection tool with high sensitivity，strong specificity，low cost，rapid and convenient，which opens a new era of molecular diagnosis. In this review，the characteristics and molecular mechanism of CRISPR/Cas13 system are introduced，the research progress of CRISPR/Cas13 technology in the detection of pathogens is comprehensively reviewed，and the future development and application prospect of CRISPR/Cas13 system are prospected.

Key words: Clustered regularly interspaced short palindromic repeat, Clustered regularly interspaced short palindromic repeat-associated protein, Pathogen

中图分类号:

R446.1

赵亚楠, 曹啟新, 闫彦, 李晓聪, 赵建平, 肖伟利. CRISPR/Cas13系统用于病原体检测的研究进展[J]. 检验医学, 2023, 38(8): 800-804.

ZHAO Yanan, CAO Qixin, YAN Yan, LI Xiaocong, ZHAO Jianping, XIAO Weili. Research progress of CRISPR/Cas13 system for pathogen detection[J]. Laboratory Medicine, 2023, 38(8): 800-804.

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CRISPR/Cas13系统用于病原体检测的研究进展

Research progress of CRISPR/Cas13 system for pathogen detection

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