RNase H依赖性PCR技术及其应用研究进展

doi:10.3969/j.issn.1673-8640.2021.012.017

摘要/Abstract

摘要：

核糖核酸酶H依赖性聚合酶链反应（rhPCR）在常规聚合酶链反应（PCR）的基础上增加了核糖核酸酶（RNase）H2和封闭式可切割rhPCR引物,消除了反应过程中引物二聚体的形成,同时大大减少了错误扩增的出现,显著提升了反应的特异性、灵敏度和重复性。rhPCR在单核苷酸多态性（SNP）、基因分型、多靶标同时检测、环境核酸检测等方面具有一定的优势。文章从rhPCR的原理、关键技术、技术优点、国内外的应用、发展前景等方面作一综述。

关键词: 核糖核酸酶H依赖性聚合酶链反应, 封闭式可切割引物, 核糖核酸酶H2

Abstract:

Ribonuclease（RNase） H-dependent polymerase chain reaction（PCR）（rhPCR） adds RNase H2 and blocked cleavable rhPCR primers based on PCR,which eliminates the formation of primer-dimers in the reaction process,greatly reduces the occurrence of error amplification,and significantly improves the specificity,sensitivity and repeatability of the reaction. The rhPCR shows certain advantages in single nucleotide polymorphism（SNP）,genotyping,multi-target simultaneous detection,environmental nucleic acid detection and so on. This review summarizes the background,principles,key technology,technical advantages,application and development prospects of rhPCR.

Key words: Ribonuclease H-dependent polymerase chain reaction, Blocked cleavable primer, Ribonuclease H2

中图分类号:

R446.1

吴新新, 韩明月, 余道军. RNase H依赖性PCR技术及其应用研究进展[J]. 检验医学, 2021, 36(12): 1277-1282.

WU Xinxin, HAN Mingyue, YU Daojun. Research progress and application of RNase H-dependent PCR technology[J]. Laboratory Medicine, 2021, 36(12): 1277-1282.

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