SARS-CoV-2核酸检测的现状和问题

doi:10.3969/j.issn.1673-8640.2021.05.019

检验医学 ›› 2021, Vol. 36 ›› Issue (5): 554-559.DOI: 10.3969/j.issn.1673-8640.2021.05.019

SARS-CoV-2核酸检测的现状和问题

黄斐¹, 张春燕², 郭玮¹, 潘柏申¹, 王蓓丽¹()

1.复旦大学附属中山医院检验科,上海 200032
2.复旦大学附属中山医院厦门医院检验科,福建厦门 361005

收稿日期:2020-09-03 出版日期:2021-05-30 发布日期:2021-05-30
通讯作者: 王蓓丽
作者简介:王蓓丽,E-mail:wang.beili1@zs-hospital.sh.cn。
黄斐,女,1990年生,学士,主管技师,主要从事分子诊断技术研究。
基金资助:
国家自然科学基金面上项目(81772263);国家自然科学基金面上项目(81972000);厦门市医疗卫生重点项目(YDZX20193502000002);复旦大学附属中山医院院内临床研究项目(2018ZSLC05);2017年“上海青年临床医技人才（临床检验专业）培养资助计划”;国家自然科学基金青年项目(81902139);上海市临床重点专科建设项目(医学检验科)

Status and problems of SARS-CoV-2 nucleic acid detection

HUANG Fei¹, ZHANG Chunyan², GUO Wei¹, PAN Baishen¹, WANG Beili¹()

1. Department of Clinical Laboratory,Zhongshan Hospital,Fudan University,Shanghai 200032,China
2. Department of Clinical Laboratory,Zhongshan Hospital,Fudan University（Xiamen Branch）,Xiamen 361015,Fujian,China

Received:2020-09-03 Online:2021-05-30 Published:2021-05-30
Contact: WANG Beili

摘要/Abstract

摘要：

新型冠状病毒肺炎（COVID-19）的持续暴发流行给全球公共卫生带来了挑战。严重急性呼吸综合征冠状病毒2（SARS-CoV-2）核酸检测是确诊COVID-19和防控疫情的主要方法。在临床实践过程中,假阴性结果会影响临床诊疗,造成疫情扩散;而假阳性结果会造成不必要的社会恐慌。文章围绕SARS-CoV-2核酸检测的影响因素,探讨临床实践中遇到的问题,以期为实验室规范和精准地开展SARS-CoV-2核酸检测提供依据。

关键词: 严重急性呼吸综合征冠状病毒2, 新型冠状病毒肺炎, 核酸检测, 假阴性, 假阳性, 样本灭活, 混检

Abstract:

The ongoing outbreak of corona virus disease 2019（COVID-19） has posed a challenge for worldwide public health. The detection of severe acute respiratory syndrome coronavirus 2（SARS-CoV-2） nucleic acid plays an important role of diagnosis,therapy,prevention and control of COVID-19. In clinical practice,false negative results may mislead clinical decision-making,leading to the spread of the epidemic,while false positive results may cause unnecessary social panic. This review focuses on the interfering factors and the status and problems of SARS-CoV-2 nucleic acid detection,so as to provide a reference for the standardization and accuracy for SARS-CoV-2 nucleic acid detection in clinical laboratory.

Key words: Severe acute respiratory syndrome coronavirus 2, Corona virus disease 2019, Nucleic acid detection, False negativity, False positivity, Sample inactivation, Sample pooling

中图分类号:

R446.1

黄斐, 张春燕, 郭玮, 潘柏申, 王蓓丽. SARS-CoV-2核酸检测的现状和问题[J]. 检验医学, 2021, 36(5): 554-559.

HUANG Fei, ZHANG Chunyan, GUO Wei, PAN Baishen, WANG Beili. Status and problems of SARS-CoV-2 nucleic acid detection[J]. Laboratory Medicine, 2021, 36(5): 554-559.

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SARS-CoV-2核酸检测的现状和问题

Status and problems of SARS-CoV-2 nucleic acid detection

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