武汉地区0~3 d新生儿末梢血网织红细胞相关参数参考区间

doi:10.3969/j.issn.1673-8640.2020.03.014

摘要/Abstract

摘要：

目的初步建立武汉市0~3 d新生儿末梢血网织红细胞（RET）6项参数的参考区间。方法选取2018年6—12月华中科技大学同济医学院附属同济医院产科病房0~3 d新生儿406名,检测其网织红细胞比例（RET%）、网织红细胞绝对值（RET#）、高荧光强度网织红细胞比例（HFR%）、中荧光强度网织红细胞比例（MFR%）、低荧光强度网织红细胞比例（LFR%）、网织红细胞血红蛋白含量（RET-He）共6项参数,用于建立生物参考区间,均采用$\bar{x}$±1.96s表示。结果不同出生天数新生儿RET 6项参数差异均有统计学意义（P<0.05）;除HFR%外,其他5项参数不同性别新生儿差异均无统计学意义（P>0.05）;武汉地区0~3 d新生儿RET 6项参数的生物参考区间为：RET%第1天2.63%~6.27%、第2天2.55%~6.35%、第3天2.06%~6.06%;RET#第1天（0.139~0.397）×10¹²/L、第2天（0.150~0.400）×10¹²/L、第3天（0.124~0.386）×10¹²/L;HFR%（男）第1天12.16%~30.97%、第2天7.64%~29.30%、第3天4.85%~20.64%;HFR%（女）第1天10.64%~27.67%、第2天6.78%~25.10%、第3天2.06%~19.26%;MFR%第1天13.48%~25.73%、第2天13.24%~26.61%、第3天11.45%~23.00%;LFR%第1天48.67%~71.07%、第2天48.24%~77.26%、第3天58.38%~83.56%;RET-He第1天29.58~37.30 pg、第2天29.25~36.23 pg、第3天27.49~34.85 pg;结论因人群所属地区和年龄不同,RET参考区间也会存在差异,本研究初步建立了武汉地区0~3 d健康新生儿RET 6项参数的参考区间,可为贫血性疾病的诊疗提供依据。

关键词: 新生儿, 网织红细胞, 参考区间

Abstract:

Objective To establish the reference intervals of 6 items of peripheral blood reticulocyte（RET） and related parameters of 0-3 d neonates in Wuhan. Methods From June 2018 to December 2018,406 0-3 d neonates in the Obstetrics Ward of Tongji Hospital of Huazhong Univerisity of Science and Technology Tongji Medical College were enrolled. A total of 6 items,including the percentage of reticulocyte（RET%）,the absolute value of reticulocyte（RET#）,high fluorescent ratio（HFR%）,middle fluorescent ratio（MFR%）,low fluorescent ratio（LFR%） and reticulocyte hemoglobin（RET-He）,were determined for establishing the reference intervals. $\bar{x}$±1.96s was used as biological reference interval. Results The 6 items showed statistical significance between different days（P<0.05）. Except for HFR%,the other 5 items showed no statistical significance between sex（P>0.05）. The reference intervals of RET and related parameters of 0-3 d neonates in Wuhan were as follows. RET%：2.63%-6.27% on the 1st day,2.55%-6.35% on the 2nd day,and 2.06%-6.06% on the 3rd day. RET#：（0.139-0.397）×10¹²/L on the 1st day,（0.150-0.400）×10¹²/L on the 2nd day,and （0.124-0.386）×10¹²/L on the 3rd day. HFR%（male）：12.16%-30.97% on the 1st day,7.64%-29.30% on the 2nd day,and 4.85%-20.64% on the 3rd day;HFR%（female）：10.64%-27.67% on the 1st day,6.78%-25.10% on the 2nd day,and 2.06%-19.26% on the 3rd day. MFR%：13.48%-25.73% on the 1st day,13.24%-26.61% on the 2nd day,and 11.45%-23.00% on the 3rd day. LFR%：48.67%-71.07% on the 1st day,48.24%-77.26% on the 2nd day,and 58.38%-83.56% on the 3rd day. RET-He：29.58-37.30 pg on the 1st day,29.25-36.23 pg on the 2nd day,and 27.49-34.85 pg on the 3rd day. Conclusions Due to different regions and ages,there are differences in the reference intervals of RET. The reference intervals of 6 items of RET and related parameters for 0-3 d neonates in Wuhan have established preliminarily,providing a reference for the diagnosis and treatment of anaemic diseases.

Key words: Neonate, Reticulocyte, Reference interval

中图分类号:

R446.1

胡卫红, 张洪波, 岳道远, 张驰. 武汉地区0~3 d新生儿末梢血网织红细胞相关参数参考区间[J]. 检验医学, 2020, 35(3): 256-259.

HU Weihong, ZHANG Hongbo, YUE Daoyuan, ZHANG Chi. Reference intervals of peripheral blood reticulocyte and related parameters of 0-3 d neonates in Wuhan[J]. Laboratory Medicine, 2020, 35(3): 256-259.

图/表 5

参考文献 13

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项目	结果	P值
RET%	4.35%±0.98%	0.215
RET#	（0.267±0.066）×10¹²/L	0.201
HFR%	17.08%±5.93%	0.845
MFR%	19.10%±3.37%	0.821
LFR%	63.82%±7.90%	0.392
RET-He	（32.59±2.08）pg	0.136

项目	结果	P值
RET%	4.35%±0.98%	0.215
RET#	（0.267±0.066）×10¹²/L	0.201
HFR%	17.08%±5.93%	0.845
MFR%	19.10%±3.37%	0.821
LFR%	63.82%±7.90%	0.392
RET-He	（32.59±2.08）pg	0.136

组别	例数	RET%/%	RET#/×10¹²/L	HFR%/%	MFR%/%	LFR%/%	RET-He/pg
男性组	222	4.29±0.97	0.264±0.066	18.19±5.99	18.95±3.37	62.86±7.85	32.56±2.22
女性组	177	4.42±0.99	0.271±0.066	15.70±5.57	19.29±3.38	65.01±7.83	32.63±1.89
t值		-1.285	-1.071	4.251	-0.981	-2.153	-0.373
P值		0.200	0.285	0.000	0.327	0.789	0.709

组别	例数	RET%/%	RET#/×10¹²/L	HFR%/%	MFR%/%	LFR%/%	RET-He/pg
男性组	222	4.29±0.97	0.264±0.066	18.19±5.99	18.95±3.37	62.86±7.85	32.56±2.22
女性组	177	4.42±0.99	0.271±0.066	15.70±5.57	19.29±3.38	65.01±7.83	32.63±1.89
t值		-1.285	-1.071	4.251	-0.981	-2.153	-0.373
P值		0.200	0.285	0.000	0.327	0.789	0.709

组别	例数	RET%/%	RET#/×10¹²/L	HFR%/%	MFR%/%	LFR%/%	RET-He/pg
0~1 d组	149	4.45±0.93	0.268±0.066	20.53±4.74	19.60±3.12	59.87±5.71	33.44±1.97
1~2 d组	146	4.45±0.97	0.275±0.064	17.33±5.29^*	19.93±3.41	62.75±7.40^*	32.74±1.78^*
2~3 d组	104	4.06±1.02^*#	0.255±0.067^*#	11.80±4.30^*#	17.23±2.95^*#	70.97±6.43^*#	31.17±1.88^*#
F值		6.206	3.417	99.434	24.715	90.820	45.537
P值		0.002	0.041	0.000	0.000	0.000	0.000