基于离子色谱法建立血清电解质参考测量程序

doi:10.3969/j.issn.1673-8640.2015.12.021

摘要/Abstract

摘要：

离子色谱是20世纪70年代发展起来的一种分析阴离子和阳离子的新型液相色谱分析技术,目前已成为分析化学领域中发展最快的分析方法之一。该技术具有快速、简便、灵敏、选择性好等诸多优点,因而被广泛地应用于工业、生物、医药、食品、临床、环境等领域。文章综述了近年来将离子色谱技术作为参考测量程序用于血清电解质测定准确性方面的研究,为离子色谱技术应用于临床生化量值溯源提供参考。

关键词: 离子色谱, 血清, 电解质, 参考测量程序

Abstract:

Ion chromatography as a new type of liquid chromatography for the analysis of anions and cations was developed in 1970. Recently, this analytical technology has become one of fastest developing methods in the field of analytical chemistry. It has been widely used in industrial, biological, medical, food, clinic and environmental samples due to its advantages of rapidness, simplicity,sensitivity and selectivity. This article summarizes the research on accuracy of ion chromatography technology as a reference measurement procedure for the determination of serum electrolytes, in order to provide valuable reference to make the analytical measurement results traceable in the field of clinical chemistry.

Key words: Ion chromatography, Serum, Electrolyte, Reference measurement procedure

中图分类号:

R446.1

邹炳德, 邹继华, 沈敏, 张曼, 吴立山, 屠敏敏. 基于离子色谱法建立血清电解质参考测量程序[J]. 检验医学, 2015, 30(12): 1250-1256.

ZOU Bingde, ZOU Jihua, SHEN Min, ZHANG Man, WU Lishan, TU Minmin. Establishment of reference measurement procedures for serum electrolytes based on ion chromatography[J]. Laboratory Medicine, 2015, 30(12): 1250-1256.

图/表 5

参考文献 35

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项目	ICP-MS	离子色谱法
准确度	<±1.0%^[33]	<±1.0%^[28]
精密度	<±1.0%^[33]	<±1.0%^[28]
检出限	ng/L	μg/L
样本分析能力	每个样本约5 min	每个样本的所有元素约30 min
能否同时检测	能,但参考测量程序一般不同时分离分析锂、钾、钠、钙、镁5种离子	能,同时分离分析锂、钾、钠、钙、镁5种离子
干扰
光(质)谱	少	-
基体	中等	样本处理后,基体干扰几乎没有
电离	很少	-
质量效应	高的对低的影响	-
同位素	有	-
可测元素数	锂、钾、钠、钙、镁、氯	锂、钾、钠、钙、镁、氯
样本用量	少	少
日常操作	容易	容易
方法试验开发	需专业技术	需专业技术
无人控制操作	能	能
易燃气体	无,但需要使用氩气	无,使用氮气提供压力
仪器费用	高	低
运行成本	高	低

项目	ICP-MS	离子色谱法
准确度	<±1.0%^[33]	<±1.0%^[28]
精密度	<±1.0%^[33]	<±1.0%^[28]
检出限	ng/L	μg/L
样本分析能力	每个样本约5 min	每个样本的所有元素约30 min
能否同时检测	能,但参考测量程序一般不同时分离分析锂、钾、钠、钙、镁5种离子	能,同时分离分析锂、钾、钠、钙、镁5种离子
干扰
光(质)谱	少	-
基体	中等	样本处理后,基体干扰几乎没有
电离	很少	-
质量效应	高的对低的影响	-
同位素	有	-
可测元素数	锂、钾、钠、钙、镁、氯	锂、钾、钠、钙、镁、氯
样本用量	少	少
日常操作	容易	容易
方法试验开发	需专业技术	需专业技术
无人控制操作	能	能
易燃气体	无,但需要使用氩气	无,使用氮气提供压力
仪器费用	高	低
运行成本	高	低

年份	RELA-A					RELA-B
年份	氯离子(mmol/L)	CV(%)	靶值(mmol/L)	偏移(%)		氯离子(mmol/L)	CV(%)	靶值(mmol/L)	偏移(%)
2012年	95.60	0.76	96.59	-0.17	107.04	0.67	108.60	-0.25
2013年	110.66	0.44	109.88	0.71	113.76	0.81	112.97	0.70

年份	RELA-A					RELA-B
年份	氯离子(mmol/L)	CV(%)	靶值(mmol/L)	偏移(%)		氯离子(mmol/L)	CV(%)	靶值(mmol/L)	偏移(%)
2012年	95.60	0.76	96.59	-0.17	107.04	0.67	108.60	-0.25
2013年	110.66	0.44	109.88	0.71	113.76	0.81	112.97	0.70