检验医学 ›› 2014, Vol. 29 ›› Issue (9): 969-973.DOI: 10.3969/j.issn.1673-8640.2014.09.022

• 技术研究与评价·论著 • 上一篇    下一篇

半自动血液推片机在高职专业教学中的使用及效果

张士化1,马少华1,王东钢2,徐坚强3,汪文娟1,陈菁1   

  1. 1. 宁波卫生职业技术学院医学检验技术教研室,浙江 宁波 315104;
    2. 浙江工商职业技术学院,浙江 宁波 315012;
    3. 宁波市医疗中心李惠利医院,浙江 宁波 315040
  • 收稿日期:2014-01-25 出版日期:2014-09-30 发布日期:2014-09-25
  • 作者简介:张士化,男,1980年生,硕士,讲师,主要从事临床检验技术和血液检验技术课程的教学和科研工作
  • 基金资助:
    2012年校级自然科学研究重点项目(XKZ201201)

Use and effect of a semiautomatic blood slide maker in the teaching of high vocational college

ZHANG Shihua1, MA Shaohua1, WANG Donggang2, XU Jianqiang3, WUANG Wenjuan1, CHEN Jing1   

  1. 1. Department of Medical Laboratory Technology, Zhejiang Ningbo College of Health Sciences, Zhejiang Ningbo 315104, China;
    2. Zhejiang Business Technology Institute, Zhejiang Ningbo 315012, China;
    3. Ningbo Medical Treatment Center LI Huili Hospital, Zhejiang Ningbo 315040, China
  • Received:2014-01-25 Online:2014-09-30 Published:2014-09-25

摘要: 目的 消除制片时人为因素的干扰,提高血液涂片(简称血涂片)的质量稳定性和规范性,促进高职生更好地学习、掌握细胞形态学知识和识别技能。方法 设计并研制出新型半自动血液推片机(简称推片机)。在血涂片制备、染色实训项目中,按照标准血涂片的条件,由行业专家和教师共同评价高职医学检验技术、卫生检验与检疫专业(简称检验、检疫专业)学生分别利用手工法和推片机法制备出的血涂片,再利用统计软件进行结果分析;记录3组(手工法制片组、手工法联合推片机法制片组及推片机法制片组)学生完成采血、制片、染色及血片观察所需时间,并比较各组的优缺点。结果 染色片检验、检疫专业教学中往往存在干扰血涂片质量的因素,ecialty推片机法制作出的合格血涂片比例显著高于手工法(P均<0.01);检验、检疫专业学生利用手工法制作的血涂片合格率无显著性变化(P>0.05);手工法联合推片机法制片组既可训练学生手工制片技能,也可为那些没有得到合格血涂片的学生制作满意的标本,增加其观察血片的时间,进一步提高了教学效果。结论 在检验专业教学中,新研制的血液推片机的使用效果满意,可作为辅助教学设备使用。

关键词: 血涂片, 半自动血液推片机, 教学, 使用, 效果

Abstract: Objective To eliminate the interference of man-made ingredient in making peripheral blood smears, and improve the quality stability and standardization of blood smears and promote high vocational college students to better study and grasp cellular morphology. Methods A new type of semiautomatic blood slide maker was designed and manufactured. When conducting the preparation and staining of a blood smear, every blood smear made by student majoring in medical laboratory technology and health inspection and quarantine technology respectively by hand and by slide maker is evaluated by experts and teachers according to the criteria of a good blood smear, and the results were analyzed statistically. In addition, the times for blood collection and its preparation, staining and microscopic examination were recorded, and the advantages and disadvantages of the 3 groups of slide making by hand, by hand allied with by slide maker and just by slide maker were compared. Results The percentage of qualified blood smears made by slide maker was much higher than that by hand in the medical laboratory technology speciality (P<0.01), whichwas the same as that in the health inspection and quarantine technology speciality (P<0.01). The percentage of qualified blood smears made by hand in these 2 specialities did not take on remarkable change (P>0.05). Besides, making blood smears by hand allied with by slide maker can not only enable all students to practise slide making, but also helped the students who had difficulty in getting a qualified blood smear, which increased their time to observe microscope and further enhanced teaching effectiveness. Conclusions The use effect of the semiautomatic blood slide maker designed and manufactured by our research group is satisfactory, which can be used as an assistant teaching equipment in the teaching of our school.

Key words: Blood smear, Semiautomatic blood slide maker, Teaching, Use, Effect

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