关键词: 马尔尼菲青霉, 骨髓涂片, 真菌联合培养, 免疫缺陷综合征

Abstract:

Objective　To investigate the laboratory diagnosis Methods　of acquired immune deficiency syndrome（AIDS） combined with penicilliosis marneffei（PSM）. Methods　A total of 257 AIDS patients were enrolled. Bone marrow and peripheral blood fungal dimorphic culture and bone marrow smear microscopy were performed for the detection of Penicillium marneffei（PM）. The data were analyzed retrospectively. Results　The 77 AIDS patients had PM， and the detection rate was 29.96%. A total of 74 patients were identified by bone marrow and peripheral blood fungal dimorphic culture， including 65 patients were positive in bone marrow， and 54 patients were positive in peripheral blood. A total of 33 patients were proved to be PM positive by bone marrow smear microscopy， including 30 patients showed positive in fungal dimorphic culture， and 3 patients showed negative but positive during therapeutic diagnosis. With the positivity of bone marrow fungal and peripheral blood fungal dimorphic culture as the good standard for PM diagnosis， the detection rate of PM by bone marrow fungal culture was 87.84%， which was significantly higher than the detection rate by peripheral blood fungal culture among AIDS patients（72.97%）. The 2 individual culture rates were significant statistically with the rate of dimorphic culture（P＜0.05）. The detection specificity for the diagnosis of PSM by bone marrow smear microscopy was 90.91%， and the sensitivity was 44.59%. Combined with bone marrow smear microscopy， the missing rate of dimorphic culture was 3.90%. Conclusions　The detection of PM by bone marrow and peripheral blood fungal dimorphic culture combined with bone marrow smear microscopy is significant in the diagnosis of AIDS combined with PM on time.

Key words: Penicillium marneffei, Bone marrow smear, Fungal dimorphic culture, Acquired immune deficiency syndrome