关键词: miR-21, miR-146a, 炎症因子, 肺炎支原体肺炎

Abstract:

Objective To investigate the expression changes of serum miR-21 and miR-146a in children with Mycoplasma pneumoniae pneumonia（MPP） and their correlation with inflammatory factors. Methods From August 2021 to September 2022，62 MPP children（MPP group） and 45 healthy children（healthy control group） were enrolled from Baotou Eighth Hospital. The general data of all the subjects were collected，and the relative expression levels of miR-21 and miR-146a in serum and the levels of inflammatory factors [interleukin（IL）-6，IL-8，IL-1β，tumor necrosis factor-alpha（TNF-α）] were determined. According to the severity of the disease，the MPP children were classified into mild group （38 cases） and severe group （24 cases）. Pearson correlation analysis was used to evaluate the correlation between serum miR-21，miR-146a，IL-6，IL-8，IL-1β and TNF-α. Receiver operating characteristic （ROC） curve was used to evaluate the efficacy of each index in the diagnosis of severe MPP. Results Serum levels of IL-6，IL-8，IL-1β，TNF-α and the relative expression level of miR-21 in MPP group were higher than those in healthy control group（P<0.001），and the relative expression level of miR-146a in serum was lower than that in healthy control group（P<0.001）. The levels of serum IL-6，IL-8，IL-1β，TNF-α and the relative expression of miR-21 in severe group were higher than those in mild group （P<0.001），and the relative expression of serum miR-146a was lower than that in mild group （P<0.001）. The miR-21 was positively correlated with IL-6，IL-8，IL-1β and TNF-α（P<0.05），while miR-146a was negatively correlated with miR-21，IL-6，IL-8，IL-1β and TNF-α（P<0.05）. IL-6，IL-8，IL-1β and TNF-α were positively correlated（P<0.05）. The areas under curves （AUC） of miR-21，miR-146a，IL-6，IL-8，IL-1β and TNF-α in the diagnosis of severe MPP were 0.794，0.770，0.829，0.837，0.866 and 0.865，respectively. The AUC of combined determination was 0.963，and the sensitivity and specificity were 87.5% and 97.4%，respectively. Conclusions Serum miR-21 and miR-146a are abnormally expressed in MPP children，and they are related to inflammatory factors，which may be used as effective indicators for clinical judgment of the severity of MPP children.

Key words: MiR-21, MiR-146a, Inflammatory factor, Mycoplasma pneumoniae pneumonia