组学技术在布鲁菌病诊断中的应用研究进展

摘要/Abstract

摘要：

布鲁菌病是一种严重的人畜共患病，其诊断一直是个难题。随着组学研究和技术的不断发展，越来越多的学者开始关注布鲁菌病的组学研究。在基因组学方面，利用测序技术获得基因组数据，可以更好地了解菌株之间的遗传差异和相似性，从而对菌株进行分型，宏基因测序已经应用于布鲁菌病的诊断。在转录组学方面，通过测序技术获取病原体在特定条件下的转录组数据，寻找对诊断布鲁菌病有价值的生物标志物。在蛋白质组学方面，通过质谱技术分析蛋白质在生物学过程中的表达、互作和调控，为开发布鲁菌病的新型诊断方法提供支持。在代谢组学方面，利用液相色谱串联质谱技术获得代谢物的代谢谱，基于代谢组数据可以鉴定代谢途径和代谢产物，更好地了解布鲁菌菌株之间的代谢差异，为布鲁菌病的诊断提供了新的方向。多组学联合可以构建出更加全面而准确的生物学信息，为布鲁菌病的诊断提供新的思路和方法。

关键词: 布鲁菌病, 组学技术, 分子诊断, 多组学联合分析

Abstract:

Brucellosis is a serious zoonotic disease，and its diagnosis has always been a difficult problem. With the development of omics research and technology，more and more researches focus on the omics of brucellosis. In terms of genomics，genomic data obtained by sequencing technology can better understand the genetic differences and similarities between strains，so as to type strains，and macro gene sequencing has been applied to the diagnosis of brucellosis in clinical work. In transcriptomics，the transcriptome data of pathogens under specific conditions are obtained by sequencing technology to find biomarkers of value for the diagnosis of brucellosis. In proteomics，the expression，interaction and regulation of proteins in biological processes are studied by mass spectrometry to provide support for new diagnostic methods of brucellosis. In terms of metabolomics，using liquid chromatography tandem mass spectrometry to obtain metabolic profiles of metabolites，analyzing metabolome data can identify metabolic pathways and metabolites，and better understand the metabolic differences between Brucella，which provides a new direction for the diagnosis of Brucella. Finally，the combination of multiple omics can construct more comprehensive and accurate biological information，and provide new ideas and methods for the diagnosis of brucellosis. This review focuses on the application of omics in the diagnosis of brucellosis.

Key words: Brucellosis, Omics, Molecular diagnosis, Multi-omics integration analysis

李孝, 李晓聪, 陈悦彤, 丁海涛. 组学技术在布鲁菌病诊断中的应用研究进展[J]. 检验医学, 2024, 39(2): 198-202.

LI Xiao, LI Xiaocong, CHEN Yuetong, DING Haitao. Research progress in the application of omics technologies for diagnosing brucellosis[J]. Laboratory Medicine, 2024, 39(2): 198-202.

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