miRNA应用于胰腺癌早期检测研究进展

doi:10.3969/j.issn.1673-8640.2014.04.023

摘要/Abstract

摘要：

胰腺癌恶性度高、预后差、死亡率高，临床通常依靠影像检测，所用诊断试剂盒特异性、准确性低，使其早期发现、鉴别诊断困难。分子诊断学是近年来新发展的临床诊断手段之一。由于miRNA在胰腺癌中的特异性表达使其可以作为胰腺癌的新型诊断标志物，,其相关的检测技术也因此得到不断发展。本文综述了胰腺癌当前临床诊断方法及诊断标志物，并对用于胰腺癌miRNA早期检测方法进行了探究。

关键词: 胰腺癌, miRNA, 分子诊断学

Abstract:

Pancreatic cancer is a malignant tumor with poor prognosis and extremely high mortality. Its diagnosis mainly relies on clinical imaging. Current diagnostic kits for pancreatic cancer diagnosis have the disadvantages of low specificity and accuracy, which results the poor early detection and diagnosis. In recent years, molecular diagnostics has been one of newly developing clinical diagnostic means. miRNA is abnormally expressed in pancreatic cancer, and it can become a novel tumor marker. The miRNA detection technology has developed continuously. This paper aims to review the current clinical diagnosis and diagnostic markers for pancreatic cancer and explore some methods used in the early detection of miRNA for pancreatic cancer.

Key words: Pancreatic cancer, miRNA, Molecular diagnostics

中图分类号:

Q503

练宓，宓现强. miRNA应用于胰腺癌早期检测研究进展[J]. 检验医学, 2014, 29(4): 395-399.

LIAN Mi, MI Xianqiang.. The research development of miRNA for the early detection of pancreatic cancer[J]. , 2014, 29(4): 395-399.

参考文献

[1] Siegel R, Naishadham D, Jemal A. Cancer statistics, 2012 [J]. CA Cancer J Clin, 2012, 62(1): 10-29.
[2] Finnegan EJ, Matzke MA. The small rna world [J]. J Cell Sci, 2003, 116(Pt 23): 4689-4693.
[3] Fukushige S, Horii A. Road to early detection of pancreatic cancer: attempts to utilize epigenetic biomarkers [J]. Cancer Lett, 2014, 342(2): 231-237.
[4] Bronstein YL, Loyer EM, Kaur H, et al. Detection of small pancreatic tumors with multiphasic helical CT[J]. AJR Am J Roentgenol, 2004, 182(3): 619-623.
[5] Harsha HC, Kandasamy K, Ranganathan P, et al. A compendium of potential biomarkers of pancreatic cancer [J]. PLoS Med, 2009, 6(4): e1000046.
[6] Isenberg G, Gouma DJ, Pisters PW. The on-going debate about perioperative biliary drainage in jaundiced patients undergoing pancreati-coduodenectomy [J]. Gastrointest Endosc, 2002, 56(2): 310-315.
[7] 张秀国,姜希宏. 胰腺癌的内镜诊治[J]. 山东医药, 2000, 40(22): 53-54.
[8] 曾彦博,王凯旋,李兆申. 胰腺癌标志物的新进展[J]. 中华胰腺病杂志, 2011, 11(6): 448-450.
[9] Koprowski H, Steplewski Z, Mitchell K, et al. Colorectal carcinoma antigens detected by hybridoma antibodies [J]. Somatic Cell Genet, 1979, 5(6): 957-971.
[10] Liu J, Gao J, Du Y, et al. Combination of plasma microRNAs with serum CA19-9 for early detection of pancreatic cancer [J]. Int J Cancer, 2012, 131(3): 683-691.
[11] Zhang Y, Li M, Wang H, et al. Profiling of 95 microRNAs in pancreatic cancer cell lines and surgical specimens by real-time PCR analysis [J]. World J Surg, 2009, 33(4): 698-709.
[12] Azmi AS, Beck FW, Bao B, et al. Aberrant epigenetic grooming of miRNAs in pancreatic cancer: a systems biology perspective[J]. Epigenomics, 2011, 3(6): 747-759.
[13] Morimura R, Komatsu S, Ichikawa D, et al. Novel diagnostic value of circulating miR-18a in plasma of patients with pancreatic cancer [J]. Br J Cancer, 2011, 105(11): 1733-1740.
[14] Kong X, Du Y, Wang G, et al. Detection of differentially expressed microRNAs in serum of pancreatic ductal adenocarcinoma patients: miR-196a could be a potential marker for poor prognosis [J]. Dig Dis Sci, 2011, 56(2): 602-609.
[15] Lennox KA, Behlke MA. A direct comparison of anti-microRNA oligonucleotide potency[J]. Pharm Res, 2010, 27(9): 1788-1799.
[16] Várallyay E, Burgyán J, Havelda Z. Detection of microRNAs by Northern blot analyses using LNA probes [J]. Methods, 2007, 43(2): 140-145.
[17] Aravin A, Tuschl T. Identification and charac-terization of small RNAs involved in RNA silencing [J]. FEBS Lett, 2005, 597(26): 5830-5840.
[18] Shi R, Chiang VL. Facile means for quantifying microRNA expression by real-time PCR[J]. Biotechniques, 2005, 39(4): 519-525.
[19] Chen PY, Manninga H, Slanchev K, et al. The developmental miRNA profiles of zebrafish as determined by small RNA cloning [J]. Genes Dev, 2005, 19(11): 1288-1293.
[20] 景花,宋沁馨,周国华. MicroRNA定量检测方法的研究进展[J]. 遗传, 2010, 32(1): 31-40.
[21] Wang X, Zhao J, Huang J, et al. The regulatory roles of miRNA and methylation on oncogene and tumor suppressor gene expression in pancreatic cancer cells [J]. Biochem Biophys Res Commun, 2012, 425(1): 51-57.
[22] Weiss FU, Marques IJ, Woltering JM, et al. Retinoic acid receptor antagonists inhibit miR-10a expression and block metastatic behavior of pancreatic cancer [J]. Gastroenterology, 2009, 137(6): 2136-2145.
[23] Volinia S, Calin GA, Liu CG, et al. A microRNA expression signature of human solid tumors defines cancer gene targets [J]. Proc Nat Acad Sci USA, 2006, 103(7): 2257-2261.
[24] Obernosterer G, Martinez J, Alenius M. Locked nucleic acid-based in situ detection of microRNAs in mouse tissue sections [J]. Nat Protoc, 2007, 2(6): 1508-1514.
[25] Yao B, Li J, Huang H, et al. Quantitative analysis of zeptomole microRNAs based on isothermal ramification amplification [J]. RNA, 2009, 15(9): 1787-1794.
[26] Raymond CK, Roberts BS, Garrett-Engele P, et al. Simple, quantitative primer-extension PCR assay for direct monitoring of microRNAs and short-interfering RNAs [J]. RNA, 2005, 11(11): 1737-1744.
[27] Jiang J, Lee EJ, Gusev Y, et al. Real-time expression profiling of microRNA precursors in human cancer cell lines [J]. Nucleic Acids Res, 2005, 33(17): 5394-5403.
[28] Lee EJ, Baek M, Gusev Y, et al. Systematic evaluation of microRNA processing patterns in tissues, cell lines, and tumors [J]. RNA, 2008, 14(1): 35-42.
[29] Tyaqi S, Kramer FR. Molecular beacons: probes that fluoresce upon hybridization [J]. Nat Biotechnol, 1996, 14(3): 303-308.
[30] Yates S, Penning M, Goudsmit J, et al. Quantitative detection of hepatitis B virus DNA by real-time nucleic acid sequence-based amplification with molecular beacon detection [J]. J Clin Microbiol, 2001, 39(10): 3656-3665.
[31] Baker MB, Bao G, Searles CD. In vitro quantification of specific microRNA using molecular beacons [J]. Nucleic Acids Res, 2012, 40(2):e13.
[32] Yin H, Zhou Y, Zhang H, et al. Electrochemical determination of microRNA-21 based on graphene, LNA integrated molecular beacon, AuNPs and biotin multifunctional bio bar codes and enzymatic assay system [J]. Biosensors Bioelectron, 2012, 33(1): 247-253.
[33] Utz PJ, Santiago JG, Kattah MG, et al. On-chip hybridization coupled with ITP based purification for fast sequence specific identification[P]. US: 20120160689, 2012-06-28.
[34] Yao Q, Zhang AM, Ma H, et al. Novel molecular beacons to monitor microRNAs in non-small-cell lung cancer [J]. Mol Cell Probes, 2012, 26(5): 182-187.