非小细胞肺癌KAI1基因和TIMP-1、MMP-9表达与临床生物学行为的关系研究

doi:10.3969/j.issn.1673-8640.2014.03.019

检验医学 ›› 2014, Vol. 29 ›› Issue (3): 279-282.DOI: 10.3969/j.issn.1673-8640.2014.03.019

非小细胞肺癌KAI1基因和TIMP-1、MMP-9表达与临床生物学行为的关系研究

朱波，赵惠柳，王英，劳明，黄昭东，刘志民，谢娟，欧超

广西医科大学附属肿瘤医院检验科，广西南宁 530021

收稿日期:2013-05-14 出版日期:2014-03-30 发布日期:2014-04-19
通讯作者: 欧超，联系电话:0771-5333422。
作者简介:朱波，男，1961年生，主任技师，主要从事肿瘤病因、机制及分子诊断研究。

Research on the expressions of KAI1 gene, TIMP-1 and MMP-9 and their correlations with clinicopathologic features of non-small cell lung cancer

ZHU Bo,ZHAO Huiliu,WANG Ying,LAO Ming,HUANG Zhaodong,LIU Zhimin,XIE Juan,OU Chao.

Department of Clinical Laboratory，The Affiliated Tumour Hospital of Guangxi Medical University，Guangxi Nanning 530021，China

Received:2013-05-14 Online:2014-03-30 Published:2014-04-19

摘要/Abstract

摘要：

目的　探讨KAI1基因和基质金属蛋白酶抑制剂1（TIMP-1）、基质金属蛋白酶9（MMP-9）在非小细胞肺癌（NSCLC）中的表达，分析其与临床生物学行为的关系。方法　应用免疫组化法检测73例NSCLC、51例相应的癌旁组织和13例非肿瘤性肺组织的KAI1和TIMP-1、MMP-9的表达，采用χ²检验和Pearson法分析三者与临床生物学行为的相关性。结果　在73例NSCLC和64例癌旁及非肿瘤组织中，KAI1和TIMP-1的阳性率分别为39.7%、74.0%和90.6%、37.5%，两者比较差异有统计学意义（P<0.05），而MMP-9阳性率为79.4%和67.1%，两者比较差异无统计学意义（P>0.05）。在NSCLC组织中，KAI1检出率在不同TNM分期、淋巴结有无转移、不同肿瘤类型中差异有统计学意义（P<0.05），TIMP-1、MMP-9的检出率在不同TNM分期、淋巴结有无转移中差异有统计学意义（P<0.05），三者检出率与年龄、性别无关；KAI1的表达与TIMP-1、MMP-9呈负相关（r=-0.463 7、-0.344 5，P<0.05）。结论　KAI1和TIMP-1、MMP-9均与肺癌的发展、转移有关，联合检测三者可成为评价NSCLC发生、发展的预后参考指标。

关键词: 1基因, 基质金属蛋白酶抑制剂1, 基质金属蛋白酶9, 肺肿瘤, 非小细胞性

Abstract:

Objective　To investigate the expressions of KAI1 gene,tissue inhibitor 1 of metalloproteinase (TIMP-1) and matrix metalloproteinase 9 (MMP-9) in non-small cell lung cancer (NSCLC) and their correlations with clinicopathologic features of NSCLC. Methods　The expressions of KAI1,TIMP-1 and MMP-9 in 73 specimens of NSCLC tissues,51 specimens of corresponding adjacent tissues and 13 specimens of non-cancerous lung tissue were determined by immunohistochemistry. Their correlations with clinicopathologic features were analyzed by χ² test and Pearson analysis. Results　In 73 specimens of NSCLC tissues and 64 cases of corresponding adjacent and non-cancerous lung tissues,the positive rates of KAI1 and TIMP-1 were 39.7%,74.0% and 90.6%,37.5% with statistical significance (P<0.05),and the positive rates of MMP-9 were 79.4%,67.1% without statistical significance (P>0.05). The expression of KAI1 was correlated to TNM stage,lymph node metastasis,histologic subtype and differentiation with statistical significance (P<0.05),and the TIMP-1 and MMP-9 were correlated to TNM stage and lymph node metastasis (P<0.05),but they were not correlated to age and sex. The expression of KAI1 was negatively correlated with the expressions of TIMP-1 and MMP-9 (r=-0.463 7,-0.344 5,P<0.05). Conclusions　The KAI1,TIMP-1 and MMP-9 are related to the development of lung cancer. The combination determination of them will be the important prognosis reference indicator for the occurrence and development of NSCLS.

Key words: 1 gene, Tissue inhibitor 1 of metalloproteinase, Matrix metalloproteinase 9, Lung neoplasm, Non-small cell

中图分类号:

R446.62

朱波，赵惠柳，王英，劳明，黄昭东，刘志民，谢娟，欧超. 非小细胞肺癌KAI1基因和TIMP-1、MMP-9表达与临床生物学行为的关系研究[J]. 检验医学, 2014, 29(3): 279-282.

ZHU Bo, ZHAO Huiliu, WANG Ying, LAO Ming, HUANG Zhaodong, LIU Zhimin, XIE Juan, OU Chao.. Research on the expressions of KAI1 gene, TIMP-1 and MMP-9 and their correlations with clinicopathologic features of non-small cell lung cancer[J]. , 2014, 29(3): 279-282.

参考文献

[1] Steeg PS. Metastasis suppressors alter the signal transduction of cancer cells[J]. Nat Rev Cancer,2003,3(1):55-63.
[2] Dong JT,Lamb PW,Rinker-Schaeffer CW,et al. KAI1,a metastasis suppressor gene for prostate can-cer on human chromosome 11p11.2[J]. Science,1995,268(5212):884-886.
[3] Liu H,Chen B,Lilly B. Fibroblasts potentiate blood vessel formation partially through secreted factor TIMP-1[J]. Angiogenesis,2008,11(3):223-234.
[4] Martin MD,Matrisian LM. The other side of MMPs: protective roles in tumor progression[J] . Cancer Metastasis Rev,2007,26(3-4):717-724.
[5] Mu Z,Wang H,Zhang J,et al. KAI1/CD82 suppresses hepatocyte growth factor-induced migration of hepatoma cells via upregulation of Sprouty 2[J]. Science China C Life Sci,2008,7(51):648-654.
[6] Kauffman EC,Robinson VL,Stadler WM,et al. Metastasis suppression: the evolving role of metastasis suppressor genes for regulating cancer cell growth at the secondary site[J]. J Urol,2003,169(3):1122-1133.
[7] Omura K,Takahashi M,Omura T,et al. Changes in the concentration of plasma matrix metalloproteinases(MMPs) and tissue inhibitor of metalloproteinases-1(TIMP-1) after total joint replacement in patients with arthritis[J]. Clin Rheumatol,2002,21(6):488-492.
[8] 蔡铭,翁鸢,王志强,等. 基质金属蛋白酶在早期肺癌中的表达[J]. 中华实验外科杂志,2010,27(5): 612-613.
[9] Jee BK,Park KM,Surendran S,et al. KAI1/CD82 suppresses tumor invasion by MMP9 inactivation via TIMP1 up-regulation in the H1299 human lung carcinoma cell line[J]. Biochem Biophys Res Commun,2006,342(2):655-661.
[10] 霍小森,孟令新,张逊.基质金属蛋白酶及其抑制剂在非小细胞肺癌组织中的表达及临床意义[J].中华肿瘤防治杂志,2007,14(10):758-761.
[11] Ow K,Delprado W,Fisher R,et al. Relationship between expression of the KAI1 metastasis suppressor and other markers of advanced bladder cancer[J]. J Pathol,2000,191(1):39-47.

[1]	张旭明, 尹伟明, 高婧. 深圳市宝安地区妊娠期甲状腺功能减退患者UGT1A1基因多态性与妊娠期合并症和不良妊娠结局的关系[J]. 检验医学, 2023, 38(7): 665-668.
[2]	蔡春艳, 上官华坤, 吴文涌, 张莹, 袁欣, 陈瑞敏. HUWE1基因变异致X连锁智力障碍临床表型和遗传学分析[J]. 检验医学, 2023, 38(2): 117-123.
[3]	王忻, 邓茜, 王娟娟, 蔡文娟, 高健, 韩艳平, 胡克非, 陈雨青. CANT1基因变异导致Desbuquois发育不良Kim变异型临床特征与遗传学分析[J]. 检验医学, 2023, 38(2): 130-136.
[4]	刘凯, 张沛茹, 谢素红, 郭林, 卢仁泉. 结直肠癌患者SorCS1基因启动子甲基化检测的临床价值[J]. 检验医学, 2022, 37(4): 330-335.
[5]	何天文, 卢建, 陈创奇, 刘顿, 丁红珂, 刘玲, 杜丽, 郑毅春, 尹爱华. 基于二代测序技术的常染色体隐性遗传性多囊肾病家系胚胎植入前遗传学分析[J]. 检验医学, 2022, 37(3): 257-263.
[6]	方忠俊, 季蔚青, 张婧娴, 吴金涛, 吴伟华, 蒋莹, 韩振格, 何东仪, 边艳琴, 沈瑜, 肖涟波, 谢东浩, 孙阳. 痛风性关节炎患者HLA-B*5801基因型携带率及相关实验室指标分析[J]. 检验医学, 2021, 36(9): 896-900.
[7]	林佳菲, 陈小松, 吴蓓颖, 蔡刚, 林琳. 21基因复发风险评分在乳腺黏液癌中的临床应用价值[J]. 检验医学, 2021, 36(7): 714-718.
[8]	吴文涌, 上官华坤, 陈瑞敏. PYCR1相关常染色体隐性皮肤松弛症患儿临床表现及遗传学特征分析[J]. 检验医学, 2021, 36(2): 135-139.
[9]	卢亚亚, 丁宇, 姚如恩, 王依柔, 张倩文, 李群, 王剑, 王秀敏, 娄丹. GRIN1突变相关发育迟缓患儿临床特征和基因突变特点分析[J]. 检验医学, 2021, 36(2): 140-146.
[10]	兰菲菲, 丁红珂, 陈延冰, 余丽华, 尹爱华. 亲子鉴定中STR基因座来源不明突变的分析[J]. 检验医学, 2021, 36(2): 185-189.
[11]	陈彬, 卢蔚薇, 徐忠玉. 血清NT-proBNP、CRP与MMP-9在儿童川崎病中的临床意义[J]. 检验医学, 2020, 35(3): 243-246.
[12]	孙康德, 张家胜, 陈旭, 虞中敏, 陈福祥. 口腔白念珠菌耐药性及毒力因子表达水平相关性研究[J]. 检验医学, 2019, 34(8): 730-735.
[13]	宦宇, 蔡徐山, 焦家军, 吴军录, 权文强, 张春利, 席芳. Beclin 1在乳腺浸润性导管癌中的表达及其与Ki67的关系[J]. 检验医学, 2019, 34(7): 595-599.
[14]	陆勇刚, 胥雨菲, 姚如恩, 李牛, 郁婷婷, 王秀敏, 沈亦平, 王剑. Sotos综合征2例报道及文献复习[J]. 检验医学, 2018, 33(4): 353-358.
[15]	林佳菲, 吴佳毅, 蔡刚, 吴蓓颖, 林琳. 459例乳腺癌患者21基因复发风险评分的临床意义[J]. 检验医学, 2017, 32(7): 590-596.

非小细胞肺癌KAI1基因和TIMP-1、MMP-9表达与临床生物学行为的关系研究

Research on the expressions of KAI1 gene, TIMP-1 and MMP-9 and their correlations with clinicopathologic features of non-small cell lung cancer

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics