关键词: PENTRA MS60血液分析仪, 不典型淋巴细胞, 显微镜分类

Abstract: Objective To evaluate the reliability of atypical lymphocyte (ALY)determination results by PENTRA MS60 hematology analyzer (PMS60), and to provide the reference for the review criteria of PMS60. Methods The 193 cases of positive ALY and 215 cases of negative ALY from fresh whole blood samples were detected by PMS60 and prepared for blood smear, and microscopy classification was performed after Wright′s staining. The 408 fresh whole blood samples were analyzed by SYSMEX XS800i hematology analyzer (XS800i) simultaneously. The ALY determination results of the 3 methods were analyzed statistically. Results Compared the results of PMS60 and XS800i, the coincidence rate of positive ALY was 89.7%, and the Kappa value was 0.793. With microscopy classification as the standard, the Kappa value of PMS60 was 0.792 with coincidence, the positive ALY sensitivity was 96.3%, the specificity was 85.3%, the accuracy was 89.7%, and the Yudon index was 0.82. After verification, the accuracy of PMS60 was 94.6%. Conclusions The ALY determination results of PMS60 are in high reliability, and it should perform microscopy classification when ALY proportion>2.5% and / or ALY absolute value>0.25×10⁹/L.

Key words: PENTRA MS60 hematology analyzer, Atypical lymphocyte, Microscopy classification