检验医学 ›› 2012, Vol. 27 ›› Issue (8): 663-666.

• 临床检验与血液学检验论著 • 上一篇    下一篇

细菌或真菌对UF-1000i尿沉渣分析仪红细胞检测的影响

  

  1. 四川大学华西医院实验医学科,四川 成都 610041
  • 收稿日期:2012-05-29 出版日期:2012-08-30 发布日期:2012-08-08
  • 通讯作者: 粟 军,联系电话:028-85422372。
  • 作者简介:郑 沁,女,1981年生,硕士,技师,主要从事临床基础检验及血液学检验工作。

Influence of different bacteria or fungi on the determination of urinary sediments by UF-1000i urinary sediment analyzer

  1. Department of Clinical Laboratory, West China Hospital of Sichuan University,Sichuan Chengdu 610041,China
  • Received:2012-05-29 Online:2012-08-30 Published:2012-08-08

摘要: 目的 探讨不同浓度不同种类细菌或真菌对UF-1000i尿沉渣分析仪(简称UF-1000i)红细胞(RBC)测定结果的影响,以对泌尿系统疾病诊断、评估提供更可靠的实验室依据。方法 健康体检者尿液标本制作成混合尿基质,将不同浓度泌尿系统感染常见细菌和真菌菌液加入制备混合尿基质,用UF-1000i检测各种浓度菌尿RBC。收集各种RBC浓度梯度病理血尿标本,加入白念珠菌菌液,调整真菌终浓度后用UF-1000i检测各种浓度菌尿RBC。每份标本同时进行显微镜镜检。结果 泌尿系统感染常见革兰阴性杆菌(大肠埃希菌、铜绿假单胞菌、肺炎克雷伯菌)、革兰阳性球菌(金黄色葡萄球菌)和低浓度的白念珠菌(<106/mL)对UF-1000i RBC测定结果无明显影响。粪肠球菌制备菌尿中,细菌浓度为109/mL时UF-1000i检测结果显示尿RBC假阳性。白念珠菌制备菌尿中,真菌浓度从106/mL开始UF-1000i检测结果显示尿RBC假阳性。真菌浓度增至108/mL时,导致血尿标本RBC定量结果显著假性增高 (P=0.006)。结论 UF-1000i具有独立的双检测通道,在很大程度上减少了细菌对尿RBC检测的干扰。但并不能有效避免高浓度真菌对RBC检测结果的影响,易造成尿RBC检测假阳性结果而影响实验室检测质量,需进行显微镜镜检校正以保证检验结果的准确性。

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关键词: UF-1000i尿沉渣分析仪, 菌尿, 红细胞计数, 血尿

Abstract: Objective To study the influence of different bacteria or fungi with different levels on the determination of urinary red blood cell(RBC) count by UF-1000i urinary sediment analyzer(UF-1000i),so as to provide more reliable laboratory evidence in the diagnosis and follow-up of urinary tract diseases.  Methods The urine RBC counts were analyzed by UF-1000i from urine mixture specimens and hematuria specimens with Blastomyces albicans,in parallel with manual microscopy respectively.  Results The Gram-negative bacilli(Escherichia coli,Klebsiella pneumonia and Pseudomonas aeruginosa) and Gram-positive cocci (Staphylococcus aureus) isolated from urine specimens of urinary tract infection and low-concentration Blastomyces albicans(<106 mL) were not impact the urine RBC count detected by UF-1000i. High concentration bacteriuria specimens(=109/mL) of Enterococcus faecalis caused the false-positive RBC count. The urine RBC count determined by UF-1000i was caused false-positive by Candida albicans, when the concentration of fungi was more than 106/mL. The concentration of Candida albicans reached to 108/mL,and the hematuria specimen RBC count would falsely increased(P=0.006).  Conclusions UF-1000i has special bacterial examination channel, and it could prevent the interference with urine RBC count obviously. However, it is hard to prevent the interference of high concentration fungi,which would cause false-positive urine RBC count. It is recommended to correct the RBC count by manual microscopy in order to provide accurate laboratory results.