检验医学 ›› 2018, Vol. 33 ›› Issue (2): 156-159.DOI: 10.3969/j.issn.1673-8640.2018.02.013

• 实验室管理_论著 • 上一篇    下一篇

上海地区厌氧菌检验室间质量评价

陈蓉, 刘学杰, 徐蓉, 葛平, 王敬华   

  1. 上海市临床检验中心,上海 200126;
  • 收稿日期:2017-11-13 出版日期:2018-02-28 发布日期:2018-03-02
  • 作者简介:null

    作者简介:陈蓉,女,1979年生,硕士,主管技师,主要从事微生物检测和质量管理工作。

  • 基金资助:
    上海市自然科学基金项目(15ZR1436100);上海市公共卫生三年行动计划(15GWZK0301);

External quality assessment for anaerobic bacterium detection in Shanghai

CHEN Rong, LIU Xuejie, XU Rong, GE Ping, WANG Jinghua   

  1. Shanghai Center for Clinical Laboratory,Shanghai 200126,China;
  • Received:2017-11-13 Online:2018-02-28 Published:2018-03-02

摘要:

目的 通过开展厌氧菌检验室间质量评价(简称室间质评)活动,了解上海地区医疗机构近年来厌氧菌检测的开展情况及检测质量。方法 收集2014—2017年参加上海市临床检验中心(SCCL)厌氧菌检验室间质评活动的反馈结果,对厌氧菌鉴定符合率进行统计分析。结果 上海地区医疗机构参加SCCL厌氧菌检验室间质评活动的实验室数由2014年的26家逐年增加到2017年的43家,增加了60.5%。2014年第1次室间质评的合格率为96.2%(25/26)(有1家医院成绩不合格),之后3年室间质评的合格率均达到100.0%。在发放的25株厌氧菌(涵盖5个属15个种)中有17株鉴定结果的符合率为100.0%,其中消化链球菌属100.0%(1/1)、丙酸杆菌属100.0%(1/1)、梭状芽孢杆菌属88.9%(8/9)、拟杆菌属46.2%(6/13)、普雷沃菌属100.0%(1/1)。结论 上海地区开展厌氧菌检验的医疗机构数在逐年增加,实验室在厌氧菌鉴定上总体的检测能力和准确率较高,但个别实验室检测能力有待提高。临床实验室的质量控制对于保证检测结果的准确性具有十分重要的作用。

关键词: 厌氧菌, 室间质量评价, 质量控制

Abstract:

Objective To study the development of anaerobic bacterium detection and its detection quality of Shanghai medical laboratories in recent years through anaerobic bacterium detection external quality assessment. Methods The feedback results of anaerobic bacterium detection external quality assessment in Shanghai Center for Clinical Laboratory(SCCL) from 2014 to 2017 were collected,and the consistency rate of anaerobic bacterium detection was analyzed. Results The number of laboratories participating SCCL anaerobic bacterium detection external quality assessment increased from 26 in 2014 to 43 in 2017(increasing rate 60.5%). Only 1 laboratory failed in the 1st external quality assessment in 2014,and the qualification rate was 96.2%(25/26). The qualification rate reached 100.0% from 2015 to 2017. A total of 25 isolates of anaerobic bacteria were delivered(covering 5 genera and 15 species). There were 17 isolates identified with the consistency rate of 100.0%,of which Peptostreptococcus 100.0%(1/1),Propionibacterium 100.0%(1/1),Clostridium 88.9%(8/9),Bacteroides 46.2%(6/13) and Prevotella 100.0%(1/1). Conclusions The number of laboratories setting anaerobic bacterium detection in Shanghai increases year by year. The overall detection ability and accuracy rate of anaerobic bacterium detection are good and high,while some laboratories' performance still should be improved. Quality control in clinical laboratories is essential to assure accuracy.

Key words: Anaerobic bacterium, External quality assessment, Quality control

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