无绿藻研究进展

doi:10.3969/j.issn.1673-8640.2017.010.021

检验医学 ›› 2017, Vol. 32 ›› Issue (10): 928-932.DOI: 10.3969/j.issn.1673-8640.2017.010.021

无绿藻研究进展

赵悦¹, 朱巍巍², 刘锦燕², 李文静¹, 王影¹, 史册¹, 项明洁²

1.上海交通大学医学院附属瑞金医院检验科,上海 200025
2.上海交通大学医学院附属瑞金医院卢湾分院放免检验科,上海 200020

收稿日期:2016-09-18 出版日期:2017-10-20 发布日期:2017-11-20
作者简介:null
作者简介：赵悦,女,1994年生,学士,主要从事酵母耐药机制和流行病学研究。

通信作者：项明洁,联系电话：021-63867463。
基金资助:
上海市黄浦区科技项目计划课题（HKW201545）

Research progress of Prototheca

ZHAO Yue¹, ZHU Weiwei², LIU Jinyan², LI Wenjing¹, WANG Ying¹, SHI Ce¹, XIANG Mingjie¹

1. Department of Clinical Laboratory,Ruijin Hospital, Shanghai Jiaotong University School of Medicine,Shanghai 200025,China
2. Radioimmunology and Clinical Laboratory,Luwan Branch,Ruijin Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200020,China

Received:2016-09-18 Online:2017-10-20 Published:2017-11-20

摘要/Abstract

摘要：

无绿藻是一种广泛存在于自然界的条件致病菌,感染动物时主要引起乳腺炎,对人的感染则主要表现为皮肤感染、鹰嘴滑囊炎和系统性感染。目前临床上仍将镜检和生化反应作为鉴定该菌的主要方法,但由于耗时长,主观影响较大,加之国内尚对无绿藻的认识不足,导致该菌的检出率较低。为提高对无绿藻的认识,文章对其生物学特性、致病性、流行病学特征、鉴定、耐药性及无绿藻病的临床表现等作一综述。

关键词: 无绿藻, 无绿藻病, 内孢子, 条件致病菌, 鉴定, 基因型

Abstract:

Prototheca is a kind of opportunistic pathogen which exists widely in nature. The clinical symptom of animal infection is mastitis,while human protothecosis is always classified to 3 forms,cutaneous lesions,olecranon bursitis and systemic infections. Currently,clinical medicine still regards morphological observation and biochemical tests as main determination methods. However,these methods are always effected by time-consuming,subjective influence and the lack of understanding in China,which results in low determination rate. For improving the knowledge of Prototheca,the biological characteristics,pathogenicity,epidemiology,identification,clinical symptoms and drug resistance of Prototheca are reviewed.

Key words: Prototheca, Protothecosis, Endospore, Opportunistic pathogen, Identification, Genotype

中图分类号:

R446.1

赵悦, 朱巍巍, 刘锦燕, 李文静, 王影, 史册, 项明洁. 无绿藻研究进展[J]. 检验医学, 2017, 32(10): 928-932.

ZHAO Yue, ZHU Weiwei, LIU Jinyan, LI Wenjing, WANG Ying, SHI Ce, XIANG Mingjie. Research progress of Prototheca[J]. Laboratory Medicine, 2017, 32(10): 928-932.

参考文献 36

[1]	KOVALYSHYN I,BRANKOV N,FERNANDEZ A P.Erythematous and edematous plaques on the bilateral extremities in an immunocompromised patient. Protothecosis[J]. Int J Dermatol,2016,55(2):e59-e61.
[2]	YEH C T,LI M C,CHUANG Y C,et al.Prototheca algaemia:a rare but fatal opportunistic infection among immunocompromised individuals[J]. Jpn J Infect Dis,2013,66(6):523-525.
[3]	RICCHI M,DE CICCO C,BUZZINI,et al. First outbreak of bovine mastitis caused by Prototheca blashkeae[J]. Vet Microbiol,2013,162(2-4):997-999.
[4]	TAKANO M,HOSHI S,NAGAI K,et al.The first case of human protothecosis caused by Prototheca zopfii in Japan[J]. J Infect Chemother,2014,20(10):647-649.
[5]	SATOH K,OOE K,NAGAYAMA H,et al.Prototheca cutis sp.nov.,a newly discovered pathogen of protothecosis isolated from inflamed human skin[J]. Int J Syst Evol Microbiol,2010,60(Pt 5):1236-1240.
[6]	TAKANO M,HOSHI S,NAGAI K,et al.The first case of human protothecosis caused by Prototheca zopfii in Japan[J]. J Infect Chemother,2014,20(10):647-649.
[7]	MARQUES S,SILVA E,CARVALHEIRA J,et al.Phenotypic characterization of mastitic Prototheca spp. isolates[J]. Res Vet Sci,2010,89(1):5-9.
[8]	LASS-FLORL,MAYR A.Human protothecosis[J].Clin Microbiol Rev,2007,20(2):230-242.
[9]	BANDARANAYAKE T D,PANIZ MONDOLFI A,PEAPER D R,et al.Prototheca wickerhamii algaemia:an emerging infection in solid organ transplant recipients[J]. Transpl Infect Dis,2015,17(4):599-604.
[10]	Protothecosis:a lethal infection due to an achlorophylous algae[J]. Clin Infect Dis,2014,59(12):iv.
[11]	MACESIC N,FLEMING S,KIDD S,et al.Protothecosis in hematopoietic stem cell transplantation:case report and review of previous cases[J]. Transpl Infect Dis,2014,16(3):490-495.
[12]	FIGUEROA C J,CAMP B J,VARGHESE G I,et al.A case of protothecosis in a patient with multiple myeloma[J]. J Cutan Pathol,2014,41(5):409-413.
[13]	TODD JR,KING JW,OBERLE A,et al.Protothecosis:report of a case with 20-year follow-up,and review of previously published cases[J]. Med Mycol,2012,50(7):673-689.
[14]	康俞莉,章强强. 无绿藻的研究进展:从基础到临床[J]. 中国真菌学杂志,2012,7(4):242-246.
[15]	HUTH N,WENKEL R F,ROSCHANSKI N,et al.Prototheca zopfii genotype 2-induced nasal dermatitis in a cat[J]. J Comp Pathol,2015,152(4):287-290.
[16]	FONT C,MASCORT J,MÁRQUEZ M,et al. Paraparesis as initial manifestation of a Prototheca zopfii infection in a dog[J]. J Small Anim Pract,2014,55(5):283-286.
[17]	MANINO P M,OLIVEIRA F,FICKEN M,et al.Disseminated protothecosis associated with diskospondylitis in a dog[J]. J Am Anim Hosp Assoc,2014,50(6):429-435.
[18]	CHOU D W,CHUNG K M,LEE C T.Prototheca wickerhamii cutaneous and systemic infections[J]. Am J Trop Med Hyg,2014,91(4):664-665.
[19]	KANO R,SOBUKAWA H,SUZUKI M,et al.Immunohistopathology of Prototheca wickerhamii in cutaneous lesions of protothecosis[J]. Med Mycol J,2014,55(1):E29-E32.
[20]	TELLO-ZAVALA M C,MERCADO-LARA A,GÓMEZ-HERNÁNDEZ N,et al. Disseminated protothecosis in a Mexican child[J]. Pediatr Infect Dis J,2013,32(12):e476-e477.
[21]	GANDHAM N R,VYAWAHARE C R,CHAUDHAURY N,et al.Onychoprotothecosis:an uncommon presentation of protothecosis[J]. Indian J Med Microbiol,2015,33(3):435-437.
[22]	ZHANG Q,LI L,YULI K,et al.A case of cutaneous protothecosis mimics eczema[J]. Mycopathologia,2015,179(1-2):163-166.
[23]	HIROSE N,NISHIMURA K,INOUE-SAKAMOTO M,et al.Ribosomal internal transcribed spacer of Prototheca wickerhamii has characteristic structure useful for identification and genotyping[J]. PLoS One,2013,8(11):e81223.
[24]	MARQUES S,HUSS V A,PFISTERER K,et al.Internal transcribed spacer sequence-based rapid molecular identific ation of Prototheca zopfii and Prototheca blaschkeae directly from milk of infected cows[J]. J Dairy Sci,2015,98(5):3001-3009.
[25]	SOBUKAWA H,IBARAKI M,KANO R,et al.Rapid molecular typing of Prototheca zopfii by high resolution melting real-time PCR(PCR-HRM)[J]. Med Mycol J,2013,54(4):341-344.
[26]	RICCHI M,CAMMI G,GARBARINO C A,et al.A rapid real-time PCR/DNA resolution melting method to identify Prototheca species[J]. J Appl Microbiol,2011,110(1):27-34.
[27]	CREMONESI P,POZZI F,RICCHI M,et al.Technical note:identification of Prototheca species from bovine milk samples by PCR-single strand conformation polymorphism[J]. J Dairy Sci,2012,95(12):6963-6968.
[28]	CAPRA E,CREMONESI P,CORTMIGLIA C,et al.Simultaneous identification by multiplex PCR of major Prototheca spp. isolated from bovine and buffalo intramammary infection and bulk tank[J]. Lett Appl Microbiol,2014,59(6):642-647.
[29]	WANG X,FU Y F,WANG R Y,et al.Identification of clinically relevant fungi and Prototheca species by rRNA gene sequencing and multilocus PCR coupled with electrospray ionization mass spectrometry[J]. PLoS One,2014,9(5):e98110.
[30]	MURUGAIYAN J,AHRHOLDT J,KOWBEL V,et al.Establishment of a matrix-assisted laser desorption ionization time-of-flight mass spectrometry database for rapid identification of infectious achlorophyllous green micro-algae of the genus Prototheca[J]. Clin Microbiol Infect,2012,18(5):461-467.
[31]	JAGIELSKI T,BUZZINI P,LASSA H,et al.Multicentre Etest evaluation of in vitro activity of conventional antifungal drugs against European bovine mastitis Prototheca spp. isolates[J]. J Antimicrob Chemother,2012,67(8):1945-1947.
[32]	MURATA M,ITO T,NAGAE K,et al.Disseminated protothecosis manifesting with multiple,rapidly-progressing skin ulcers:successful treatment with amphotericin B[J]. Eur J Dermatol,2015,25(2):208-209.
[33]	KNOX J,COLOE S V,PERERA C,et al.Prototheca wickerhamii olecranon bursitis successfully treated with adjunctive systemic itraconazole[J]. Pathology,2015,47(4):388-391.
[34]	FARRAG H A,A-KARAM EI-DIN A,MOHAMED EI-SAYED Z G,et al. Microbial colonization of irradiated pathogenic yeast to catheter surfaces:relationship between adherence,cell surface hydrophobicity,biofilm formation and antifungal susceptibility. A scanning electron microscope analysis[J]. Int J Radiat Biol,2015,91(6):519-527.
[35]	KWIECINSKI J.Biofilm formation by pathogenic Prototheca algae[J]. Lett Appl Microbiol,2015,61(6):511-517.
[36]	MORANDI S,CREMONESI P,CAPRA E,et al.Molecular typing and differences in biofilm formation and antibiotic susceptibilities among Prototheca strains isolated in Italy and Brazil[J]. J Dairy Sci,2016,99(8):6436-6445.

无绿藻研究进展

Research progress of Prototheca

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献 36

相关文章 15

编辑推荐

Metrics

[1]	田媛媛, 贾雄飞. 肠球菌的有益性和致病性[J]. 检验医学, 2023, 38(11): 1101-1105.
[2]	朱丰村, 曾云祥, 金晓立, 曾添, 黄天鹏, 林枝, 周林双. 204株碳青霉烯类耐药肠杆菌目细菌耐药性分析[J]. 检验医学, 2023, 38(1): 60-65.
[3]	李可可, 肖扬. 凝血因子Ⅴ与出血和血栓的相关性研究进展[J]. 检验医学, 2022, 37(4): 396-399.
[4]	刘聪聪, 李佳萍, 周宏伟. 2套质谱系统临床微生物鉴定性能评估[J]. 检验医学, 2022, 37(11): 1084-1088.
[5]	杨溪, 宋文琪, 董方, 孟庆盈, 甄景慧, 周为. 革兰染色与MALDI-TOF MS在快速鉴定儿童阳性血培养病原菌种类中的应用[J]. 检验医学, 2022, 37(10): 969-973.
[6]	王婧婧, 吴林清, 陈如寿, 王玉丰. 耐万古霉素肠球菌耐药基因、转座子结构和多位点序列分析[J]. 检验医学, 2021, 36(9): 891-895.
[7]	方忠俊, 季蔚青, 张婧娴, 吴金涛, 吴伟华, 蒋莹, 韩振格, 何东仪, 边艳琴, 沈瑜, 肖涟波, 谢东浩, 孙阳. 痛风性关节炎患者HLA-B*5801基因型携带率及相关实验室指标分析[J]. 检验医学, 2021, 36(9): 896-900.
[8]	余佳佳, 李媛睿, 刘瑛. SDS和SAP前处理联合MALDI-TOF MS在报阳血培养样本快速病原菌鉴定中的价值[J]. 检验医学, 2021, 36(8): 864-868.
[9]	焦瑞宝, 唐吉斌, 周佳丽, 朱娟娟, 陈继中. GPⅠa基因G873A、C807T位点在心脑血管疾病和糖尿病患者中的多态性分布[J]. 检验医学, 2021, 36(6): 581-585.
[10]	顾玉微, 王成云, 顾萍, 潘秋辉, 王静, 陈广洁. ABO等位基因调控区突变致抗原弱表达原因分析[J]. 检验医学, 2021, 36(6): 637-641.
[11]	李育敏, 蔡钦泉, 覃俊龙, 金潇, 陈亚琼, 莫云均, 李悦, 张秀明. 3种β缺失型地中海贫血基因型与表型分析[J]. 检验医学, 2021, 36(6): 642-645.
[12]	丁毅伟, 韩志海. MALDI-TOF MS在人工关节置换术后病原菌快速鉴定中的价值[J]. 检验医学, 2021, 36(6): 656-661.
[13]	陈蓉, 张敏敏, 夏启航, 钱诚凯, 崔琳, 刘学杰, 徐蓉, 王庆忠, 葛平, 黄维纲, 王敬华. 上海地区真菌培养鉴定及体外药物敏感性试验室间质量评价结果分析[J]. 检验医学, 2021, 36(4): 441-443.
[14]	乔玉莉, 黄宗帅, 田月如, 关明, 蒋晓飞. 血培养瓶增菌法快速鉴定和快速药物敏感性试验可行性分析[J]. 检验医学, 2021, 36(3): 270-274.
[15]	兰菲菲, 丁红珂, 陈延冰, 余丽华, 尹爱华. 亲子鉴定中STR基因座来源不明突变的分析[J]. 检验医学, 2021, 36(2): 185-189.