关键词: 8-羟基脱氧鸟苷酸, 丙二醛, 谷胱甘肽过氧化物酶, 氧化应激, 脑卒中

Abstract:

Objective To investigate the change and clinical significance of serum oxidative stress indicators in patients with cerebral stroke. Methods Serum 8-hydroxydeoxyguanosine（8-OHdG）,malondialdehyde（MDA） and glutathione peroxidase（GSH-Px） in 168 patients with cerebral stroke,including 86 patients with ischemic stroke（IS） and 82 patients with intracerebral hemorrhage（ICH）,and 93 healthy subjects（healthy control group） were determined by enzyme-linked immunosorbent assay（ELISA）. Results Serum 8-OHdG levels {median（M）[quartile interval（P₂₅-P₇₅）]} in IS and ICH groups were 17.4（8.3-32.1） and 9.3（6.6-21.8） ng/mL,and MDA levels [M（P₂₅-P₇₅）] were 5.6（4.4-11.2）and 7.2（5.5-13.0）ng/mL,which were significantly higher than those in healthy control group [4.3（2.1-6.9）and 2.5（1.1-4.5）ng/mL]（P<0.05）,while serum GSH-Px levels [M（P₂₅-P₇₅）] were 479.5（259.2-861.7） and 277.3（128.6-379.6）ng/mL,which were significantly lower than those in healthy control group [751.2（567.2-904.7）ng/mL]（P<0.05）. The levels of 8-OHdG and GSH-Px were higher in IS group than in ICH group（P<0.05）,and MDA levels had no statistical significance between the 2 groups（P>0.05）. The 8-OHdG levels in patients with cerebral stroke were positively correlated with MDA and GSH-Px（r=0.21 and 0.59,P<0.01）,and the GSH-Px level had no correlation with MDA level（r=0.11,P>0.05）. Conclusions The levels of serum 8-OHdG,MDA and GSH-Px are sensitive oxidative stress indicators for patients with cerebral stroke. The levels of serum 8-OHdG and GSH-Px correlate with the severity of cerebral stroke,which are significant in the differential diagnosis of cerebral stroke.

Key words: 8-Hydroxydeoxyguanosine, Malondialdehyde, Glutathione peroxidase, Oxidative stress, Cerebral stroke