血浆网膜素-1检测在2型糖尿病下肢血管病变患者中的临床应用

doi:10.3969/j.issn.1673-8640.2014.07.006

摘要/Abstract

摘要：

目的　探讨2型糖尿病（T2DM）下肢血管病变患者血浆网膜素-1的变化及与高敏C反应蛋白（hs-CRP）的关系。方法　选取93例T2DM患者，根据踝肱指数（ABI）分为T2DM伴下肢血管病变组（简称T2DM+LLVD组，51例）和T2DM不伴下肢血管病变组（简称SDM组，42例），以健康体检者40名作为正常对照（NC）组，采用酶联免疫吸附（ELISA）法检测各组空腹血浆网膜素-1浓度，并分析网膜素-1与hs-CRP及其他指标的关系。结果　SDM组和T2DM+LLVD组血浆网膜素-1水平分别为（18.30±5.17）、（12.93±4.60）ng/mL，均明显低于NC组[（23.94±7.08 ng/mL]（P均＜0.01）；T2DM+LLVD组血浆网膜素-1水平明显低于SDM组（P＜0.01）。T2DM+LLVD组患者血浆网膜素-1水平与hs-CRP、收缩压（SBP）、甘油三酯（TG）、空腹血糖（FBG）、胰岛素抵抗指数（HOMA-IR）和颈动脉内膜中层厚度（CIMT）呈明显负相关[r值分别为－0.405、－0.260、－0.148、－0.209、－0.307、－0.419，P值分别为0.000、0.046、0.039、0.005、0.012、0.003]，与ABI呈明显正相关（r＝0.358，P＝0.007）。hs-CRP、CIMT和ABI分别是影响T2DM伴下肢血管病变患者血浆网膜素-1水平的独立相关因素。结论　血浆网膜素-1水平可能与T2DM下肢血管病变及其动脉硬化密切相关。

关键词: 网膜素-1, 糖尿病, 2型, 下肢血管病变, 超敏C反应蛋白, 动脉粥样硬化

Abstract:

Objective　To investigate the changes of plasma Omentin-1 in type 2 diabetes mellitus（T2DM） patients with lower limb vascular disease（LLVD） and the relationship between Omentin-1 and high-sensitivity C-reactive protein（hs-CRP）. MethodsA total of 93 patients with T2DM were enrolled and classified into T2DM patients accompanied with LLVD（51cases， T2DM+LLVD group） and simple T2DM patients（42 cases， SDM group）， according to ankle brachial index ABI）， and 40 healthy subjects were enrolled as healthy controls（NC group）. Fasting plasma Omentin-1 levels were determined by enzyme-linked immunosorbent assay（ELISA）. The relationships between plasma Omentin-1 levels， hs-CRP and other metabolic parameters were also analyzed. ResultsPlasma Omentin-1 levels were significantly higher in SDM group [（18.30±5.17）ng/mL] and T2DM+LLVD group[（12.93±4.60）ng/mL] than that in NC group[（23.94±7.08）ng/mL]（P＜0.01）. Plasma Omentin-1 levels were significantly higher in T2DM+LLVD group than that in SDM group（P＜0.01）.Plasma Omentin-1 levels in T2DM+LLVD group were negatively related to hs-CRP， systolic blood pressure（SBP）， triglyceride（TG）， fasting blood glucose（FBG）， the index of homeostasis model of insulin resistance（HOMA-IR）and carotid intima-media thickness（CIMT）（r＝－0.405，－0.260，－0.148，－0.209，－0.307 and －0.419，P＝0.000，0.046，0.039，0.005，0.012 and 0.003）， and were correlated positively with ABI（r＝0.358，P＝0.007）. The hs-CRP， CIMT and ABI were the independent risk factors of plasma Omentin-1 in T2DM+LLVD group. ConclusionsPlasma Omentin-1 levels may be closely correlated with T2DM LLVD and atherosclerosis.

Key words: Omentin-1, Type 2 diabetes mellitus, Lower limb vascular disease, High-sensitivity C-reactive protein, Atherosclerosis

中图分类号:

R446.1

孙庆艳，李秉云. 血浆网膜素-1检测在2型糖尿病下肢血管病变患者中的临床应用[J]. 检验医学, 2014, 29(7): 718-722.

SUN Qingyan,LI Bingyun.. Clinical application of plasma Omentin-1 determination in type 2 diabetes mellitus patients with lower limb vascular disease[J]. , 2014, 29(7): 718-722.

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