手工MGIT液体培养结核分枝杆菌污染再处理分析

doi:10.3969/j.issn.1673-8640.2014.05.016

检验医学 ›› 2014, Vol. 29 ›› Issue (5): 498-500.DOI: 10.3969/j.issn.1673-8640.2014.05.016

手工MGIT液体培养结核分枝杆菌污染再处理分析

吴学兵¹, 陈碧英¹, 江渊², 李志兰¹, 陆彬³, 阚立新⁴

1.上海市第七人民医院医学检验科, 上海 200137;
2.上海市疾病预防控制中心结核病防治科, 上海 200336 ;
3.上海市松江区中心医院检验科, 上海 201600;
4.Departments of Neurology, Northwestern University Feinberg Medical School, Chicago, IL 60611, USA

收稿日期:2013-06-03 出版日期:2014-05-30 发布日期:2014-05-27
作者简介:吴学兵, 男, 1964年生, 学士, 主任技师, 主要从事临床检验实验室质量管理工作。

Analysis on the contamination of MGIT liquid culture manual method for Mycobacterium tuberculosis by decontamination

WU Xuebing¹, CHEN Biying¹, JIANG Yuan², LI Zhilan¹, LU Bin³, KAN Lixin⁴.

1.Department of Clinical Laboratory, Shanghai Seventh People′s Hospital, Shanghai 200137, China;
2. Department of Tuberculosis Prevention and Treatment, Shanghai Center for Disease Control and Prevention, Shanghai 200336, China;
3. Department of Clinical Laboratory, Central Hospital of Shanghai Songjiang District, Shanghai 201600, China;
4.Department of Neurology, Northwestern University Feinberg Medical School, Chicago 60611, USA

Received:2013-06-03 Online:2014-05-30 Published:2014-05-27

摘要/Abstract

摘要： 目的分析手工分枝杆菌培养管(MGIT)液体培养法检测结核分枝杆菌中的污染再处理效果。方法收集疑似新发肺结核初诊的1 088例患者的痰标本, 所有标本均分别用液体MGIT法、固体改良罗氏培养基L-J法及痰涂片抗酸染色进行检测, 统计MGIT法首次培养总污染率和去污染后再次培养的总污染率, 并和固体L-J法污染率进行比较。结果 MGIT法的首次污染率涂片阳性(简称涂阳)组为11.24%, 涂片阴性(简称涂阴)组为7.29%, 总污染率为7.90%;L-J法涂阳组的污染率为2.96%, 涂阴组为3.80%, 总污染率为3.68%, 两者之间差异有统计学意义(P均<0.05)。去污染后涂阳组的再污染率为0.59%, 涂阴组为4.68%, 总污染率为4.04%, 与L-J法相应各组间差异无统计学意义(P均＞0.05);去污染后再培养涂阳组污染率低于涂阴组(P<0.05)。结论 MGIT法可去污染再培养, 以降低污染率, 特别是涂阴标本应调整和增加前处理的消化时间或灭菌剂的浓度, 以控制首次MGIT法的污染率, 减少再处理去污染次数。建议临床医生应在控制其他细菌感染后再留标本做结核分枝杆菌培养。

关键词: 结核分枝杆菌, 分枝杆菌培养管液体培养, 固体L-J培养, 污染, 去污染

Abstract: Objective To analyze the contamination and treatment effect of Mycobacterium grow indicate tube (MGIT) liquid culture manual method for the detection of Mycobacterium tuberculosis. Methods A total of 1 088 sputum specimens from patients suspected pulmonary tuberculosis diagnosed preliminary were collected, and all specimens were respectively determined by MGIT method, improved L-J medium base (L-J) method and sputum smear acid-fast staining, and the contamination rates of MGIT method for the first time and after decontamination were compared with those of L-J method. Results In MGIT method for the first time, the contamination rate of smear positive group was 11.24%, that of smear negative group was 7.29%, and the total contamination rate was 7.90%. In L-J method, the contamination rate of smear positive group was 2.96%, that of smear negative group was 3.80%, and the total contamination rate was 3.68%. The corresponding differences were statistically significant (P<0.05). The contamination rate of smear positive group after decontamination was lower than that of smear negative group, there was statistical significance (P<0.05). Conclusions The MGIT method can be used to decontaminate in contaminated culture and reduce the contamination rate, especially smear negative specimens should be adjusted and increased the concentration of digestion time or sterilized agent before pretreatment, in order to control the first time MGIT method′s contamination rate, reduce the number of decontamination, and recommend that clinicians should control the other bacterial infection before leaving specimens for tuberculosis culture.

Key words: Mycobacterium tuberculosis, Mycobacterium grow indicate tube liquid culture manual method, L-T medium base, Contamination , Decontamination

中图分类号:

R446.5

吴学兵, 陈碧英, 江渊, 李志兰, 陆彬, 阚立新. 手工MGIT液体培养结核分枝杆菌污染再处理分析[J]. 检验医学, 2014, 29(5): 498-500.

WU Xuebing, CHEN Biying, JIANG Yuan, LI Zhilan, LU Bin, KAN Lixin.. Analysis on the contamination of MGIT liquid culture manual method for Mycobacterium tuberculosis by decontamination[J]. , 2014, 29(5): 498-500.

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手工MGIT液体培养结核分枝杆菌污染再处理分析

Analysis on the contamination of MGIT liquid culture manual method for Mycobacterium tuberculosis by decontamination

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