人精子顶体内顶体蛋白酶和透明质酸酶对男性生育力的影响

doi:10.3969/j.issn.1673-8640.2014.03.014

检验医学 ›› 2014, Vol. 29 ›› Issue (3): 258-261.DOI: 10.3969/j.issn.1673-8640.2014.03.014

人精子顶体内顶体蛋白酶和透明质酸酶对男性生育力的影响

马晓萍¹，高晓勤¹，杨燕平²，杨喆²

1.遵义医药高等专科学校组织胚胎学教研室，贵州遵义 563002；2.贵阳医学院组织胚胎学教研室，贵州贵阳 550004

收稿日期:2013-04-18 出版日期:2014-03-30 发布日期:2014-04-19
通讯作者: 高晓勤，联系电话:0852-8925881。
作者简介:马晓萍，女，1984年生，硕士，讲师，主要研究方向为男性生殖学、组织学与胚胎学。
基金资助:
贵州省科学技术基金项目(黔科合J字[2011]2273号)

The influence of acrosome enzyme and hyaluronidase in human sperm acrosome on male fertility

MA Xiaoping¹,GAO Xiaoqin¹,YANG Yanping²,YANG Zhe

1.Department of Histology and Embryology,Zunyi Medical and Pharmaceutical College,Guizhou Zunyi 563002,China;
2.Department of Histology and Embryology,Guiyang Medical College,Guizhou Guiyang 550004,China

Received:2013-04-18 Online:2014-03-30 Published:2014-04-19

摘要/Abstract

摘要：

目的　探讨人精子顶体内酶对男性生育力的影响。方法　参照世界卫生组织标准方法对52例不育症患者、12名正常生育者[正常生育组，精子密度为（50～100）×10⁹/L、精子活动率为60%～90%]进行精液常规分析。不育组按精子密度不同分为B1（<20×10⁹/L）、C1（20×10⁹～40×10⁹/L）及D1（>40×10⁹/L）3组；按精子活动率不同分为B2（<30%）、C2（30%～50%）及D2（>50%）3 组]。采用改良明胶底物膜法和改良透明质酸钠-明胶底物膜法分别检测精子顶体内顶体蛋白酶（ACE）活性（ACE阳性反应率、ACE活性强度）和透明质酸酶（HYD）活性（HYD阳性反应率、HYD活性强度）。结果　精子ACE活性（ACE阳性反应率、ACE活性强度）与精子密度、活动率、形态畸形率明显相关[相关系数（r）分别为0.797、0.867，0.831、0.860和－0.625、－0.546，P均<0.01]；精子HYD活性（HYD阳性反应率、HYD活性强度）与精子密度、活动率、形态畸形率明显相关[r值分别为0.832、0.855，0.842、0.830和－0.625、－0.554，P均<0.01]。不育各组精子形态畸形率明显高于正常生育组（P<0.01）。C1组、D1组和C2组、D2组ACE活性（ACE阳性反应率、ACE活性强度）、HYD活性（HYD阳性反应率、HYD活性强度）明显低于正常生育组（P<0.01）。各组酶活性随精子密度及活动率增加而上升。结论　精子ACE、HYD活性的检测可作为一种有效的判断生育力的检测指标。

关键词: 顶体蛋白酶, 透明质酸酶, 生育力, 男性

Abstract:

Objective　To investigate the influence of enzymes in human sperm acrosome on male fertility. Methods　According to the World Health Organization standard method,52 cases were enrolled as infertile group,and 12 cases were enrolled as normal control group[sperm density (50-100)×10⁹/L and sperm motility 60%-90%],and semen routine analysis was performed. According to the density of sperm,the infertile group was classified into infertile group B1(<20×10⁹/L),C1(20×10⁹ -40×10⁹/L) and D1(>40×10⁹/L),and according to the motility of sperm,the infertile group was classified into infertile group B2(<30%),C2(30%-50%) and D2(>50%). The positive reaction rate and activity intensity of acrosome enzyme (ACE) were detected by fixed gelatin substrate film technique . The positive reaction rate and activity intensity of hyaluronidase (HYD) were detected by modified sodium hyaluronate-gelatin membrane. Results　There was a significant correlation between the positive reaction rate and activity intensity of ACE with sperm density,sperm motility and sperm deformity rate [correlation coefficients (r) were 0.797,0.867; 0.831,0.860 and -0.625,-0.546,P<0.01]. There was a significant correlation between the positive reaction rate and activity intensity of HYD with sperm density,sperm motility and sperm deformity rate (r were 0.832,0.855; 0.842,0.830 and -0.625,-0.554,P<0.01). The sperm deformity rate in infertile group was higher than that in normal control group (P<0.01). The positive reaction rates and activity intensities of ACE and HYD in infertile group C1,D1 and C2,D2 were significantly lower than those in normal control group (P<0.01). The activity of enzymes increased with the sperm density and motility. Conclusions　The detections of sperm ACE and HYD are effective parameters for the analysis of male fertility.

Key words: Acrosome enzyme, Hyaluronidase, Fertility, Male

中图分类号:

Q556

马晓萍，高晓勤，杨燕平，杨喆. 人精子顶体内顶体蛋白酶和透明质酸酶对男性生育力的影响[J]. 检验医学, 2014, 29(3): 258-261.

MA Xiaoping, GAO Xiaoqin, YANG Yanping, YANG Zhe. The influence of acrosome enzyme and hyaluronidase in human sperm acrosome on male fertility[J]. , 2014, 29(3): 258-261.

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人精子顶体内顶体蛋白酶和透明质酸酶对男性生育力的影响

The influence of acrosome enzyme and hyaluronidase in human sperm acrosome on male fertility

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