广泛耐药鲍曼不动杆菌氨基糖苷类药物获得性耐药基因研究

doi:10.3969/j.issn.1673-8640.2013.11.010

检验医学 ›› 2013, Vol. 28 ›› Issue (11): 1008-1011.DOI: 10.3969/j.issn.1673-8640.2013.11.010

广泛耐药鲍曼不动杆菌氨基糖苷类药物获得性耐药基因研究

王玉月，史伟峰，周军

苏州大学附属第三医院检验科，江苏常州 213003

收稿日期:2012-12-14 出版日期:2013-11-30 发布日期:2013-12-20
作者简介:王玉月，女，1971年生，学士，副主任技师，主要从事临床微生物检验与细菌耐药基因研究。

The study of acquired resistant genes against aminoglycosides in extensively drug-resistant Acinetobacter baumannii

WANG Yuyue,SHI Weifeng,ZHOU Jun.

Department of Clinical Laboratory, the Third Affiliated Hospital of Soochow University,Jiangsu Changzhou 213003,China

Received:2012-12-14 Online:2013-11-30 Published:2013-12-20

摘要/Abstract

摘要：

目的　了解重症监护病房(ICU)患者分离的广泛耐药(XDR)-鲍曼不动杆菌(AB)对氨基糖苷类药物获得性耐药基因情况。方法　用phoenix^TM-100全自动细菌鉴定药物敏感性分析仪对AB进行细菌鉴定和药物敏感性试验，gyrA和parC基因扩增测序确定AB。聚合酶链反应(PCR)测定20株XDR-AB的10种氨基糖苷类修饰酶基因、6种16SrRNA甲基化酶基因和外排泵adeB基因，并用DNA测序比对。结果　20株XDR-AB中，氨基糖苷类修饰酶基因aac(3)-Ⅰ、aac(6′)-Ⅰb、ant(3″)-Ⅰ、aph(3′)-Ⅰ检出率分别为90.0%、30.0%、95.0%、95.0%，而aac(3)-Ⅱ、aac(6′)-Ⅰad、aac(6′)-Ⅱ、ant(2″)-Ⅰ、ant(4′)-Ⅰ及aph(3′)-Ⅵa基因均未检出。armA型16SrRNA甲基化酶基因和外排泵adeB基因检出率均为100%。结论　20株XDR-AB均携带了armA和adeB基因，同时aac(3)-Ⅰ、ant(3″)-Ⅰ和aph(3)-Ⅰ检出率较高，提示ICU分离的XDR-AB对氨基糖苷类药物高水平耐药可能与携带的耐药基因有关。

关键词: 广泛耐药鲍曼不动杆菌, 氨基糖苷类修饰酶, 获得性耐药基因, 序列分析

Abstract:

Objective　To investigate the situation of acquired resistant genes against aminoglycosides in extensively drug-resistant (XDR) Acintobacter baumannii (AB) isolated from intensive care unit (ICU) patients. Methods　Bacterial identification and susceptibility tests of AB were performed by phoenix^TM-100 automatic bacterial identification and susceptibility analyzer, and the AB identification was confirmed by gyrA and parC gene amplification sequencing. For the 20 isolates of XDR-AB, 10 aminoglycoside modifying enzyme genes, 6 16S rRNA methylase genes and efflux pump adeB genes were detected by polymerase chain reaction(PCR) and verified and compared by DNA sequencing. Results　Of the 20 isolates of XDR-AB, the detection rates of aac(3)-Ⅰ,aac(6′)-Ⅰb,ant(3″)-Ⅰ and aph(3′)-Ⅰ genes were 90.0%,30.0%,95.0% and 95.0%,respectively.However, aac(3)-Ⅱ,aac(6′)-Ⅰad,aac(6′)-Ⅱ,ant(2″)-Ⅰ,ant(4′)-Ⅰand aph(3′)- Ⅵa genes were not found.In addition,the armA 16S rRNA methylase gene and efflux pump adeB genes existed in all of the 20 isolates, and the detection rates were 100%. Conclusions　All of the 20 isolates of XDR-AB carry not only armA and adeB genes but also a large amount of aac(3)-Ⅰ,ant(3″)-Ⅰand aph(3)-Ⅰgenes.It indicates that the high-level resistance to aminoglycosides in XDR-AB isolated from ICU may be associated with the resistant genes.

Key words: Extensively drug-resistant Acintobacter baumannii, Aminoglycoside modifying enzyme, Acquired resistant gene, Sequencing analysis

中图分类号:

R446.5

王玉月，史伟峰，周军. 广泛耐药鲍曼不动杆菌氨基糖苷类药物获得性耐药基因研究[J]. 检验医学, 2013, 28(11): 1008-1011.

WANG Yuyue,SHI Weifeng,ZHOU Jun.. The study of acquired resistant genes against aminoglycosides in extensively drug-resistant Acinetobacter baumannii[J]. , 2013, 28(11): 1008-1011.

参考文献

[1] 王辉,郭萍,孙宏莉, 等. 碳青霉烯类耐药的不动杆菌分子流行病学及其泛耐药的分子机制[J]. 中华检验医学杂志, 2007,29(12):1066-1073.
[2] 刘东华,李从荣. 多药耐药与泛耐药鲍氏不动杆菌的临床分布及耐药性研究[J]. 中华医院感染学杂志, 2012, 22(2):402-403.
[3] Falagas ME,Koletsi PK, Bliziotis IA. The diversity of definitions of multidrug-resistant (MDR) and pandrug-resistant (PDR) Acinetobacter baumannii and Pseudomonas aeruginosa[J]. J Med Microbiol, 2006, 55(Pt 12):1619-1629.
[4] 周月清,陆开来, 糜祖煌. 鲍曼不动杆菌 TEM 基因及氨基糖苷类修饰酶基因初步研究[J]. 中华检验医学杂志, 2005,28(2):200-202.
[5] 马序竹,吕媛,李耘,等. 头孢哌酮/舒巴坦 (2: 1, 1: 1) 联合米诺环素对亚胺培南耐药鲍曼不动杆菌的体外抗菌作用[J]. 中国临床药理学杂志, 2010,26(3):167-170.
[6] 姜梅杰,秦霞. 多重耐药鲍曼不动杆菌中β-内酰胺类相关耐药基因的研究[J].中华临床医师杂志(电子版),2012,23(6):7809-7811.
[7] 糜祖煌,秦玲. 多药耐药鲍氏不动杆菌对 5 类抗菌药物耐药机制研究[J]. 中华医院感染学杂志, 2008,18(7):901-904.
[8] Muir ME,Wallace BJ. Isolation of mutants of Escherichia coli uncoupled in oxidative phosphorylation using hypersensitivity to streptomycin[J]. Biochim Biophys Acta, 1979,547(2):218-229.
[9] Bryan LE,Van Den Elzen HM. Effects of membrane-energy mutations and cations on streptomycin and gentamicin accumulation by bacteria: a model for entry of streptomycin and gentamicin in susceptible andresistant bacteria[J]. Antimicrobial Agents Chemother, 1977,12(2):163-177.
[10] 黄支密,糜祖煌,储秋菊,等. 鲍曼不动杆菌 16S rRNA甲基化酶基因及氨基糖苷类修饰酶基因研究[J]. 中华微生物学和免疫学杂志, 2009,28(8):727-728.
[11] 糜祖煌,秦玲. 泛耐药铜绿假单胞菌 16S rRNA 甲基化酶、氨基糖苷类修饰酶基因研究[J]. 中华医院感染学杂志, 2009,18(12):1656-1658.
[12] Ruzin A,Keeney D, Bradford PA. AdeABC multidrug efflux pump is associated with decreased susceptibility to tigecycline in Acinetobacter calcoaceticus-Acinetobacter baumannii complex[J]. JAntimicrob Chemother, 2007,59(5):1001-1004

广泛耐药鲍曼不动杆菌氨基糖苷类药物获得性耐药基因研究

The study of acquired resistant genes against aminoglycosides in extensively drug-resistant Acinetobacter baumannii

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 11

编辑推荐

Metrics

[1]	王婧婧, 吴林清, 陈如寿, 王玉丰. 耐万古霉素肠球菌耐药基因、转座子结构和多位点序列分析[J]. 检验医学, 2021, 36(9): 891-895.
[2]	徐炜新, 孙杰. 多位点序列分析法在白念珠菌性阴道炎唑类耐药菌株分子流行病学研究中的运用[J]. 检验医学, 2018, 33(3): 233-238.
[3]	吴蓉, 吕玉江, 戴俊华, 相芬芬, 孔倩倩, 钱宁, 张贵留, 杨玉玲, 陈丽春, 康向东. 产ESBLs大肠埃希菌对氨基糖苷类药物的耐药性及其耐药基因序列分析[J]. 检验医学, 2016, 31(11): 959-964.
[4]	田斌, 段绩辉, 徐明忠, 张兵, 廖瑜, 申晓君, 文岚. 三例输入性卵形疟的实验室诊断[J]. 检验医学, 2014, 29(8): 851-855.
[5]	姚冬婷,应春妹,郑冰. 微卫星多态性和多位点序列分析技术在光滑念珠菌基因分型中的应用评估[J]. 检验医学, 2014, 29(6): 593-596.
[6]	熊燏,钱士匀. 载脂蛋白E基因三种分型检测方法临床效能对比评价[J]. 检验医学, 2013, 28(9): 789-792.
[7]	芮球红，廖于峰，陈燕敏. ICU产ESBLs大肠埃希菌氨基糖苷类修饰酶基因的分析[J]. 检验医学, 2013, 28(5): 452-454.
[8]	张东华;于德敏;金根娣;姜节洪;张欣欣. PCR-荧光探针法检测乙型肝炎病毒YMDD变异与DNA序列分析法的比较[J]. 检验医学, 2011, 26(01): 5-8.
[9]	由然;孙立颖. 耐阿米卡星铜绿假单胞菌氨基糖苷类耐药基因及其基因型研究[J]. 检验医学, 2010, 25(05): 341-344.
[10]	俞云松;陈亚岗;宋秀兰;周伟琳;马亦林. 多重耐药肺炎克雷伯菌β-内酰胺酶编码基因分子生物学研究[J]. 检验医学, 2001, 16(z1): 21-25.
[11]	朱浩华;梁启美;褚倩;项军. 400例献血员TT病毒检测与分析[J]. 检验医学, 2001, 16(05): 290-291.