Expression changes of serum miR-21 and miR-146a in children with Mycoplasma pneumoniae pneumonia and their correlation with inflammatory factors

doi:10.3969/j.issn.1673-8640.2024.08.012

Abstract

Abstract:

Objective To investigate the expression changes of serum miR-21 and miR-146a in children with Mycoplasma pneumoniae pneumonia（MPP） and their correlation with inflammatory factors. Methods From August 2021 to September 2022，62 MPP children（MPP group） and 45 healthy children（healthy control group） were enrolled from Baotou Eighth Hospital. The general data of all the subjects were collected，and the relative expression levels of miR-21 and miR-146a in serum and the levels of inflammatory factors [interleukin（IL）-6，IL-8，IL-1β，tumor necrosis factor-alpha（TNF-α）] were determined. According to the severity of the disease，the MPP children were classified into mild group （38 cases） and severe group （24 cases）. Pearson correlation analysis was used to evaluate the correlation between serum miR-21，miR-146a，IL-6，IL-8，IL-1β and TNF-α. Receiver operating characteristic （ROC） curve was used to evaluate the efficacy of each index in the diagnosis of severe MPP. Results Serum levels of IL-6，IL-8，IL-1β，TNF-α and the relative expression level of miR-21 in MPP group were higher than those in healthy control group（P<0.001），and the relative expression level of miR-146a in serum was lower than that in healthy control group（P<0.001）. The levels of serum IL-6，IL-8，IL-1β，TNF-α and the relative expression of miR-21 in severe group were higher than those in mild group （P<0.001），and the relative expression of serum miR-146a was lower than that in mild group （P<0.001）. The miR-21 was positively correlated with IL-6，IL-8，IL-1β and TNF-α（P<0.05），while miR-146a was negatively correlated with miR-21，IL-6，IL-8，IL-1β and TNF-α（P<0.05）. IL-6，IL-8，IL-1β and TNF-α were positively correlated（P<0.05）. The areas under curves （AUC） of miR-21，miR-146a，IL-6，IL-8，IL-1β and TNF-α in the diagnosis of severe MPP were 0.794，0.770，0.829，0.837，0.866 and 0.865，respectively. The AUC of combined determination was 0.963，and the sensitivity and specificity were 87.5% and 97.4%，respectively. Conclusions Serum miR-21 and miR-146a are abnormally expressed in MPP children，and they are related to inflammatory factors，which may be used as effective indicators for clinical judgment of the severity of MPP children.

Key words: MiR-21, MiR-146a, Inflammatory factor, Mycoplasma pneumoniae pneumonia

CLC Number:

R446.1

ZHAI Bin, HAN Mei. Expression changes of serum miR-21 and miR-146a in children with Mycoplasma pneumoniae pneumonia and their correlation with inflammatory factors[J]. Laboratory Medicine, 2024, 39(8): 787-792.

Figures/Tables 7

References 23

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名称	上游引物（5'~3'）	下游引物（5'~3'）
miR-21	ACACTCCAGCTCGGTAGCTTATCACACTGA	TGCTCTCGTCCAGTCG
miR-146a	CTGGACTGCAAGGAGGGGTC	GCAGACTGAAAAAGTTCTT
U6	CGCAAGGATGA-CACG	GAGCAGGCTGGAGAA

名称	上游引物（5'~3'）	下游引物（5'~3'）
miR-21	ACACTCCAGCTCGGTAGCTTATCACACTGA	TGCTCTCGTCCAGTCG
miR-146a	CTGGACTGCAAGGAGGGGTC	GCAGACTGAAAAAGTTCTT
U6	CGCAAGGATGA-CACG	GAGCAGGCTGGAGAA

组别	例数	性别		年龄/岁	BMI/（kg·m^-2）	既往呼吸系统疾病史/ [例（%）]
组别	例数	男/[例（%）]	女/[例（%）]	年龄/岁	BMI/（kg·m^-2）	既往呼吸系统疾病史/ [例（%）]
MPP组	62	33（53.23）	29（46.77）	6.74±2.00	15.53±4.17	32（51.61）
正常对照组	45	25（55.56）	20（44.44）	6.49±1.93	15.76±4.26	16（35.56）
统计值		0.057		0.656	-0.279	2.718
P值		0.811		0.513	0.781	0.099

组别	例数	性别		年龄/岁	BMI/（kg·m^-2）	既往呼吸系统疾病史/ [例（%）]
组别	例数	男/[例（%）]	女/[例（%）]	年龄/岁	BMI/（kg·m^-2）	既往呼吸系统疾病史/ [例（%）]
MPP组	62	33（53.23）	29（46.77）	6.74±2.00	15.53±4.17	32（51.61）
正常对照组	45	25（55.56）	20（44.44）	6.49±1.93	15.76±4.26	16（35.56）
统计值		0.057		0.656	-0.279	2.718
P值		0.811		0.513	0.781	0.099

组别	例数	IL-6/（ng·L^-1）	IL-8/（ng·L^-1）	IL-1β/（ng·L^-1）	TNF-α/（ng·L^-1）	miR-21	miR-146a
MPP组	62	57.23±14.02	76.84±18.56	31.87±8.16	22.91±9.24	2.76±0.60	0.65±0.17
正常对照组	45	14.27±3.68	40.85±12.01	8.03±2.37	8.56±2.52	1.07±0.31	1.13±0.30
t值		23.058	12.159	21.774	11.646	17.229	-9.650
P值		<0.001	<0.001	<0.001	<0.001	<0.001	<0.001