Determination and clinical significance of NLRP3，CTRP6 and IL-1β in patients with gestational diabetes mellitus

doi:10.3969/j.issn.1673-8640.2023.10.006

Abstract

Abstract:

Objective To investigate the clinical significance of serum NOD-like receptor protein（NLRP3），C1q/tumor necrosis factor-related protein 6（CTRP6） and interleukin 1 beta（IL-1β） in patients with gestational diabetes mellitus（GDM）. Methods Totally，145 patients with GDM in Nantong Maternal and Child Health Care Hospital from January 2018 to December 2020 were enrolled as GDM group，and 145 healthy pregnant women were enrolled as control group. Serum NLRP3，CTRP6，IL-1β levels and fasting insulin（FINS），glycated hemoglobin A_1c（HbA_1c），fasting plasma glucose（FPG），homa-beta cell function index（HOMA-β），homeostasis model assessment of insulin resistance（HOMA-IR） levels were determined. According to the pregnancy outcomes，they were classified into adverse pregnancy outcome group and normal pregnancy outcome group. Pearson correlation analysis was used to evaluate the correlation between various indicators in GDM group. Receiver operating characteristic（ROC） curve was used to evaluate the effectiveness of all the indicators in determining adverse pregnancy outcomes. Results Compared with control group，CTRP6，NLRP3，IL-1β，FINS，HbA_1c，FPG and HOMA-IR levels in GDM group were increased（P<0.05），while HOMA-β was decreased（P<0.05）. CTRP6，NLRP3 and IL-1β were positively correlated with FINS，HbA_1c，FPG and HOMA-IR（P<0.05），and they were negatively correlated with HOMA-β levels（P<0.05）. Compared with normal pregnancy group，CTRP6，NLRP3，IL-1β，FINS，HbA_1c，FPG and HOMA-IR were increased in adverse pregnancy group（P<0.05），while HOMA-β was decreased（P<0.05）. The areas under curves（AUC） of FINS，HbA_1c，FPG，HOMA-β and HOMA-IR for judging adverse pregnancy outcome were all <0.7，while the AUC of CTRP6，NLRP3 and IL-1β for judging adverse pregnancy outcome were 0.846，0.802 and 0.871，respectively. The AUC of combined determination was 0.931. Conclusions The serum levels of CTRP6，NLRP3 and IL-1β in patients with GDM are increased，and they are closely related to insulin resistance and blood glucose levels. The combined determination of CTRP6，NLRP3 and IL-1β has high clinical value in predicting adverse pregnancy outcome.

Key words: NOD-like receptor protein, C1q/tumor necrosis factor-related protein 6, Interleukin 1 beta, Gestational diabetes mellitus

CLC Number:

R446.1

QIAN Linyu, LIANG Weifang, TANG Sichen. Determination and clinical significance of NLRP3，CTRP6 and IL-1β in patients with gestational diabetes mellitus[J]. Laboratory Medicine, 2023, 38(10): 936-940.

Figures/Tables 6

References 16

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组别	例数	年龄/岁	孕周/周	孕次/次	BMI/ （kg/m²）	TG/ （mmol/L）	TC/ （mmol/L）	LDL-C/ （mmol/L）	HDL-C/ （mmol/L）
GDM组	145	28.7±4.9	27.9±1.7	1.74±0.56	26.17±1.94	2.51±0.39	4.61±0.74	2.46±0.29	1.43±0.28
对照组	145	28.1±4.2	27.6±1.3	1.68±0.52	25.94±1.87	2.60±0.42	4.49±0.78	2.51±0.32	1.36±0.38
t值		1.121	1.688	0.945	1.028	1.891	1.344	1.394	1.786
P值		0.264	0.093	0.345	0.305	0.060	0.180	0.164	0.075

组别	例数	年龄/岁	孕周/周	孕次/次	BMI/ （kg/m²）	TG/ （mmol/L）	TC/ （mmol/L）	LDL-C/ （mmol/L）	HDL-C/ （mmol/L）
GDM组	145	28.7±4.9	27.9±1.7	1.74±0.56	26.17±1.94	2.51±0.39	4.61±0.74	2.46±0.29	1.43±0.28
对照组	145	28.1±4.2	27.6±1.3	1.68±0.52	25.94±1.87	2.60±0.42	4.49±0.78	2.51±0.32	1.36±0.38
t值		1.121	1.688	0.945	1.028	1.891	1.344	1.394	1.786
P值		0.264	0.093	0.345	0.305	0.060	0.180	0.164	0.075

组别	例数	CTRP6/ （μg/L）	NLRP3/ （pg/mL）	IL-1β/ （pg/mL）	FINS/（mU/L）	HbA_1c/%	FPG/（mmol/L）	HOMA-β	HOMA-IR
GDM组	145	506.43±119.52	236.80±57.14	27.21±6.74	10.59±1.42	6.28±0.61	5.49±0.31	35.98±9.39	4.34±0.61
对照组	145	391.76±101.98	156.20±32.12	17.69±4.24	7.31±1.54	4.63±0.69	4.47±0.22	61.08±12.15	1.98±0.54
t值		8.789	14.817	14.397	18.855	21.573	32.311	19.683	34.883
P值		<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

组别	例数	CTRP6/ （μg/L）	NLRP3/ （pg/mL）	IL-1β/ （pg/mL）	FINS/（mU/L）	HbA_1c/%	FPG/（mmol/L）	HOMA-β	HOMA-IR
GDM组	145	506.43±119.52	236.80±57.14	27.21±6.74	10.59±1.42	6.28±0.61	5.49±0.31	35.98±9.39	4.34±0.61
对照组	145	391.76±101.98	156.20±32.12	17.69±4.24	7.31±1.54	4.63±0.69	4.47±0.22	61.08±12.15	1.98±0.54
t值		8.789	14.817	14.397	18.855	21.573	32.311	19.683	34.883
P值		<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001	<0.001

项目	FINS	HbA_1c	FPG	HOMA-β	HOMA-IR
CTRP6
r值	0.391	0.334	0.429	-0.226	0.338
P值	<0.001	<0.001	<0.001	0.014	<0.001
NLRP3
r值	0.335	0.317	0.372	-0.354	0.228
P值	<0.001	<0.001	<0.001	<0.001	0.012
IL-1β
r值	0.394	0.422	0.347	-0.359	0.325
P值	0.022	0.018	0.009	<0.001	<0.001